Cell death in the development of the posterior cortex in male and female rats

Journal of Comparative Neurology - Tập 436 Số 1 - Trang 32-41 - 2001
Joseph L. Nuñez1, David M. Lauschke2, Janice M. Juraska2,1
1Neuroscience Program, University of Illinois at Champaign-Urbana, Urbana, Illinois 61801
2Department of Psychology, University of Illinois at Champaign-Urbana, Champaign, Illinois 61820

Tóm tắt

AbstractWork from our laboratory has shown that adult male rats have 19% more neurons than female rats in the binocular region and 18% more in the monocular region of the primary visual cortex (Reid and Juraska [1992] J Comp Neurol 321:448–455; Nuñez et al., [1999] Soc Neurosci Abstr 25:229). In the current experiment, we investigated whether cell death in male and female rats (postnatal days 2–35) contributes to the formation of these differences. Using stereological techniques, we investigated neuron density along with pyknotic and apoptotic (TdT‐mediated deoxyuridine triphosphate nick end‐labeled) cell density in the developing posterior cortex (future primary visual cortex). Although no sex differences in neuronal density were found in early development, we observed a differential time course of cell death between the sexes. Consistent with earlier reports, males displayed a rapid rise in cell death, with a peak on day 7 followed by a sharp decline to negligible levels by day 15. Females, however, displayed moderate peaks of cell death on days 7 and 11, with the persistence of low‐to‐modest levels until day 25. Similar patterns were obtained from both pyknotic and apoptotic cell quantification. Also, a formula was developed to estimate the percentage of cells that die during development and the amount of time a dying cell is visible. This study demonstrates that there is a prolonged period of cell death in the posterior cortex of developing female rats that appears to result in more cell death in females than males. This may be an important mechanism by which the sex difference in adult neuron number is created. J. Comp. Neurol. 436:32–41, 2001. © 2001 Wiley‐Liss, Inc.

Từ khóa


Tài liệu tham khảo

10.1006/hbeh.1994.1027

10.1016/0168-0102(96)01070-X

10.1016/0092-8674(92)90531-G

Bayer SA, 1991, Neocortical development

10.1242/dev.122.4.1165

10.1002/(SICI)1096-9861(19980622)396:1<39::AID-CNE4>3.0.CO;2-J

10.1016/0166-2236(95)93933-O

Choi DW, 1996, Ischemia‐induced neuronal apoptosis, Curr Opin Cell Biol, 6, 667

10.1007/BF00174615

10.1126/science.6474175

10.1016/0006-8993(96)00298-3

10.1016/S0304-3940(97)00445-X

10.1016/0306-4522(90)90281-8

10.1016/0301-0082(92)90029-E

10.1016/0304-3940(94)90210-0

10.1002/neu.480230908

10.1016/0301-0082(89)90017-8

10.1126/science.6828866

10.1083/jcb.119.3.493

10.1002/cne.901930214

10.1002/(SICI)1097-4695(199912)41:4<585::AID-NEU13>3.0.CO;2-#

10.1016/0006-8993(75)90618-6

Gundersen HJG, 1988, The new stereological tools: disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis, AMPIS, 96, 857

10.1002/(SICI)1097-4695(199903)38:4<491::AID-NEU5>3.0.CO;2-E

Heumann D, 1977, Postnatal development of the mouse cerebral neocortex II. Quantitative cytoarchitectonics of visual and auditory areas, J Hirnforsch, 18, 483

Heumann D, 1978, Postnatal development of the mouse cerebral neocortex IV. Evolution of the total cortical volume, of the population of neurons and glial cells, J Hirnforsch, 19, 385

Hu Z, 1997, The in vivo time course for elimination of adrenalectomy‐induced apoptotic profiles from the granule cell layer of the rat hippocampus, J Neurosci, 17, 3981, 10.1523/JNEUROSCI.17-11-03981.1997

10.1016/0165-3806(85)90019-7

10.1002/cne.902120210

10.1002/cne.901830203

10.1002/cne.903350109

10.1016/S0165-3806(97)00080-1

Leuba G, 1978, Postnatal development of the mouse cerebral neocortex III. Some dynamical aspects, J Hirnforsch, 19, 301

10.1002/(SICI)1097-4547(19980215)51:4<497::AID-JNR9>3.0.CO;2-7

10.1023/A:1007076206828

10.1159/000111246

10.1006/hbeh.1994.1033

10.1016/S0165-3806(98)00125-4

Nuñez JL, 1999, Sex and hormone manipulation effects upon the volume and number of neurons in the rat primary visual cortex, Soc Neurosci Abstr, 25, 229

10.1016/S0165-3806(00)00126-7

10.1002/j.1460-2075.1993.tb06042.x

10.1002/cne.902390102

10.1146/annurev.ne.14.030191.002321

10.1159/000054371

10.1016/0959-4388(94)90036-1

10.1126/science.8235590

10.1002/cne.903210311

Rubin LL, 1994, The molecular mechanisms of neuronal apoptosis, Trends Neurosci, 4, 696

10.1002/cne.902040402

10.1016/0165-3806(92)90158-S

10.1523/JNEUROSCI.19-04-01179.1999

10.1073/pnas.92.24.11110

10.1002/cne.903630209

Strom RC, 1998, Cell production and cell death in the generation of variation in neuron number, J Neurosci, 18, 9948, 10.1523/JNEUROSCI.18-23-09948.1998

10.1523/JNEUROSCI.19-23-10357.1999

10.1523/JNEUROSCI.17-03-01075.1997

10.1083/jcb.137.2.459

10.1159/000017434

10.1111/j.1460-9568.1995.tb01045.x

10.1002/ar.1092310411

10.1016/0165-3806(88)90237-4

10.1016/S0304-3940(99)00942-8

Thông tin
Thông tin xuất bản

Journal of Comparative Neurology

Tập 436 Số 1

32-41

Thông tin tác giả