Cell biology of hematoporphyrin derivative (hpd)

Lasers in Surgery and Medicine - Tập 2 Số 3 - Trang 261-266 - 1983
Michael W. Berns1, Marie C. Wilson1, Robert Burns1, Alan G. Wile2, P. M. Rentzepis3
1Departments of Developmental and Cell Biology, Murray Hill, New Jersey
2Department of Surgery, Murray Hill, New Jersey
3University of California, Irvine, and Bell Laboratories, Murray Hill, New Jersey

Tóm tắt

AbstractTissue culture cells were treated with hematoporphyrin derivative (HPD) and analyzed for (1) HPD uptake, (2) HPD washout, and (3) fluorescence changes. The absorption peaks were the same for HPD in solution and HPD bound to cells. HPD was taken up by all cell types rapidly within the first 10 hours of exposure, and leveled off by 20 hours of exposure. HPD came out of all cell types to control levels by 72 hours after removal of the HPD bathing solution. The HPD appeared to come out of the malignant 3‐T‐12 cells more slowly than from the parental (non‐malignant) 3‐T‐3 cells. The fluorescence spectrum of HPD‐treated cells changed markedly from the time that the bathing HPD solution was removed from the cells. As time progressed the weakly fluorescent 590‐nm peak increased greatly and the 615‐nm and 630‐nm peaks decreased significantly.

Từ khóa


Tài liệu tham khảo

Lipson RL, 1961, Hematoporphyrin derivative: A new aid for endoseopic detection of malignant disease, J Thorac Cardiovasc Surg, 42, 623, 10.1016/S0022-5223(19)32560-7

Dougherty TJ, 1978, Photoradiation therapy for the treatment of malignant tumors, Cancer Res, 38, 2628

KatoH KonakaC OnoJ MatushimaY NishimiyaK:Laser Photoradiation with hematoporphyrin derivative in lung cancer. Proceedings of the Fourth Congress of the International Society for Laser Surgery Tokyo November 23–27 1981.

Berns MW, 1982, In vitro cellular effects of hematoporphyrin derivative (HPD), Cancer Res, 42, 2325

10.1080/09553008114550261

10.1016/0009-2797(81)90166-6

Kessel D, 1981, Transport and binding of hematoporphyrin derivative and related porphyrins by murine leukemia L1210 cells, Cancer Res, 41, 1318

10.1002/jcp.1040670207

Thông tin
Thông tin xuất bản

Lasers in Surgery and Medicine

Tập 2 Số 3

261-266

Thông tin tác giả