Catalytic activity and immunochemical quantification of hepatic cytochrome P-450 in β-naphthoflavone and isosafrol treated rainbow trout (Oncorhynchus mykiss)

Fish Physiology and Biochemistry - Tập 9 Số 3 - Trang 189-197 - 1991
Malin Celander1, Lars Förlin1
1Department of Zoophysiology, University of Göteborg, Göteborg, Sweden

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Andersson, T., Pesonen, M. and Johansson, C. 1985. Differential induction of cytochrome P-450-dependent monooxygenase, epoxide hydrolase, glutathione transferase and UDP glucuronosyl transferase activities in the liver of the rainbow trout by β-naphthoflavone or clophen A50. Biochem. Pharmacol. 34: 3309–3314.

Burke, M.D. and Mayer, R.T. 1974. Ethoxyresorufin: direct fluorometric assay of a microsomal O-dealkylation which is preferentially inducible by 3-methylcholanthrene. Drug Metab. Dispos. 2: 583–588.

Celander, M., Fölin, L., and Andersson, T. 1989. Cytochrome P-450 mediated O-dealkylation of 7-alkoxycoumarins in liver microsomes from rainbow trout (Salmo gairdneri). Fish Physiol. Biochem. 6: 199–205.

Celander, M. and Förlin, L. 1989. Immunochemical quantitation of β-naphthoflavone-inducible cytochrome P-450 in rainbow trout and perch.In Cytochrome P-450 Biochemistry and Biophysics. pp. 710–713. Edited by I. Schuster. Taylor and Francis, London.

Forlin, L. 1980. Effects of Clophen A50, 3-methylcholanthrene, pregnenolone-16α-carbonitrile, and phenobarbital on the hepatic microsomal cytochrome P-450-dependent monooxygenase system in rainbow trout,Salmo gairdneri, of different age and sex. Toxieol. Appl. Pharmacol. 54: 420–430.

Forlin, L., Andersson, T., Goksoyr, A. and Zhang, Y. 1986. Hepatic microsomal cytochromes P-450 from BNF-treated perch. Mar. Environ. Res. 24: 112.

Förlin, L., Goksoyr, A., Andersson, T. and Celander, M. 1989. Catalytic and immunochemical studies of cytochrome P-450 in perch exposed to bleached kraft mill effluents.In Cytochrome P-450 Biochemistry and Biophysics. pp. 702–705. Edited by I. Schuster. Taylor and Francis, London.

Goksoyr, A. 1985. Purification of hepatic microsomal cytochromes P-450 from β-naphthoflavone-treated Atlantic cod (Gadus morhua), a marine teleost fish. Biochem. Biophys. Acta 840: 409–417.

Goksoyr, A., Husoy, A.-M., Larsen, H.E., Klungsoyr, J., Wilhelmsen, S., Brevik, E.M., Andersson, T., Celander, M., Pesonen, M. and Forlin, L., 1989. Evaluation of biochemical responses to environmental contaminants in flatfish from the Hvaler archipelago in Norway. Mar. Environ. Res. 28: 51–55.

Gonzalez, F.J., Mackenzie, P.I., Kimura, S. and Nebert, D.W. 1984. Isolation and characterization of full-length mouse cDNA and genomic clones of 3-methylcholanthrene-inducible cytochrome, P1-450 and P3-450. Gene 29: 281–292.

Gooch, J.W., Elskus, A.A., Kloepper-Sams, P.J., Hahn, M.E. and Stegeman, J.J. 1989. Effects oforto- and non-orto-substituted polychlorinated biphenyl congeners on the hepatic monooxygenase system in seup (Stenotomus chrysops). Toxicol. Appl. Pharmacol. 98: 422–433.

Greenlee, W.F. and Poland, A. 1978. An improved assay of 7-ethoxycoumarin-O-deethylase activity: induction of hepatic enzyme activity in C57B1 6J and DBA 2J mice by phenobarbital, 3-methylcholanthrene and 2,3,7,8-tetrachlorodibenzo-p-dioxin. J. Pharmacol. Exp. Ther. 205: 596–605.

Haux, C. and Förlin, L., 1988. Biochemical methods for detecting effects of contaminants on fish. Ambio 17: 376–380.

Heilmann, L.J., Sheen, Y.-Y., Bigelow, S.W. and Nebert, D.W. 1988. Trout P4501A1: cDNA and deduced protein sequence, expression in liver, and evolutionary significance. DNA 7: 379–387.

Kleinow, K.M., Melancon, M.J. and Lech, J.J. 1987. Biotransformation and induction: implications for toxicity, bioaccumulation and monitoring of environmental xenobioties in fish. Environ. Health Perspec. 71: 105–119.

Kloepper-Sams, P.J., Park, S.S., Gelboin, H.V. and Stegeman, J.J. 1987. Specificity and cross-reactivity of monoclonal and polyclonal antibodies against cytochrome P-450E of the marine fish scup. Arch. Biochem. Biophys. 253: 268–278.

Klotz, A.V., Stegeman, J.J. and Walsh, C. 1983. An aryl hydrocarbon hydroxylating hepatic cytochrome P-450 from the marine fishStenotomus crysops. Arch. Biochem. Biophys. 226: 578–592.

Kupfer, D., Miranda, G.K. and Bulger, W.H. 1981. A facile assay for 2-hydroxylation of estradiol by liver microsomes. Anal. Biochem. 116: 27–34.

Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of Bacteriophage T4. Nature, Lond. 227: 680–685.

Leaver, M.J., Burke, M.D., George, S.G., Davies, J.M. and Raffaelli, D. 1988. Induction of cytochrome P-450 monooxygenase activities in plaice by ‘model’ inducers and drilling muds. Mar. Environ. Res. 24: 27–30.

Lowry, O.H., Rosebrough, N.J., Farr, A.I. and Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275.

Masters, B.S.S. Williams, C.H. and Kamin, H. 1967. The preparation and properties of microsomal TPNH-cytochrome c reductase from pig liver.In Methods in Enzymology 10: 565–573. Edited by Estabrook and M.E. Pullman. Academic Press, New York.

Matsubara, T., Prough, R.A., Burke, M.D. and Estabrook, R.W. 1974. The preparation of microsomal fractions of rodent respiratory tract and their characterization. Cancer 34: 2196–2203.

Miranda, C.L., Wang, J.-L., Chang, H.-S. and Buhler, D.R. 1990. Multiple effects of 3,4,5,3′,4′,5′-hexachlorobiphenyl administration on hepatic cytochrome P450 isozymes and associated mixed-function oxidase activities in rainbow trout. Biochem. Pharmacol. 40: 388–390.

Nebert, D.W. and Gonzalez, F.J. 1987. P-450 genes: structure, evolution, and regulation. Ann. Rev. Biochem. 56: 943–993.

Ortiz De Montellano, P.R. 1986. Cytochrome P-450. Structure, Mechanism, and Biochemistry. Plenum Press, New York.

Payne, J.F., Fancey, L.L., Rahimtula, A.D. and Porter, E.I., 1987. Review and perspective on the use of mixed-function oxygenase enzymes in biological monitoring. Comp. Biochem. Physiol. 86C: 233–345.

Ryan, D.E., Thomas, P.E., Reik, L.M. and Levin, W. 1982. Purification, characterization and regulation of five rat hepatic microsomal cytochrome P-450 isozymes. Xenobiotica 12: 727–744.

Stegeman, J.J. 1989. Cytochrome P450 forms in fish: catalytie, immunological and sequence similarities. Xenobiotica 19: 1093–1110.

Stegeman, J.J., Teng, F.Y. and Snowberger, E.A. 1987. Induced cytochrome P450 in winter flounder (Pseudopleuronectes americanus) from coastal Massachusetts evaluated by catalytic assay and monoclonal antibody probes. Can. J. Fish. Aquat. Sci. 44: 1270–1277.

Towbin, H., Stachelin, T. and Gordon, J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Nat. Acad. Sci. U.S.A. 76: 4350–4354.

Varanasi, U., Collier, T.K., Williams, D.E. and Buhler, D.R. 1986. Hepatie cytochrome P-450 isozymes and aryl hydrocarbon hydroxylase in English sole (Parophys vetulus). Biochem. Pharmacol. 35: 2967–2971.

Warner, M. 1982. Catalytic activity of partially purified renal 25-hydroxyvitamin D hydroxylases from vitamin D-deficient and vitamin D-replete Rats. J. Biol. Chem. 257: 12995–13000.

Williams, D.E. and Buhler, D.R. 1982. Purification of cytochromes P-448 from β-naphthoflavone-treated rainbow trout. Biochim. Biophs. Acta 717: 398–404.

Williams, D.E. and Buhler, D.R. 1984. Benzo(a)pyrenehydroxylase catalyzed by purified isozymes of cytochrome P-450 from β-naphthoflavone-fed rainbow trout. Biochem. Pharmacol. 33: 3743–3753.

Wray, W., Boulikas, T. Wray, V.P. and Hancock, R. 1981. Silver staining of proteins in polyacrylamide gels. Anal. Biochem. 118: 197–203.

Zhang, Y.S., Goksoyr, A., Andersson, T. and Förlin, L. 1991. Initial purification and characterization of hepatic microsomal cytochrome P-450 from BNF-treated perch (Perca fluvatilis). Comp. Biochem. Physiol. 98B: 97–103.