Carboxylates and alcohols production in an autotrophic hydrogen‐based membrane biofilm reactor

Biotechnology and Bioengineering - Tập 118 Số 6 - Trang 2338-2347 - 2021
Diana C. Calvo1,2, Aura Ontiveros‐Valencia1,3, Rosa Krajmalnik‐Brown4,1,2, César I. Torres1,5, Bruce E. Rittmann1,2
1Biodesign Swette Center for Environmental Biotechnology Arizona State University Tempe Arizona USA
2School of Sustainable Engineering and the Built Environment Ira A. Fulton Schools of Engineering, Design Annex Tempe Arizona USA
3Department of Environmental Sciences Instituto Potosino de Investigación Científica y Tecnológica San Luis Potosí Mexico
4Biodesign Center for Health Through Microbiome Arizona State University Tempe Arizona USA
5School for Engineering of Matter Transport and Energy, Ira A. Fulton Schools of Engineering Tempe Arizona USA

Tóm tắt

AbstractMicrobiological conversion of CO2 into biofuels and/or organic industrial feedstock is an excellent carbon‐cycling strategy. Here, autotrophic anaerobic bacteria in the membrane biofilm reactor (MBfR) transferred electrons from hydrogen gas (H2) to inorganic carbon (IC) and produced organic acids and alcohols. We systematically varied the H2‐delivery, the IC concentration, and the hydraulic retention time in the MBfR. The relative availability of H2 versus IC was the determining factor for enabling microbial chain elongation (MCE). When the H2:IC mole ratio was high (>2.0 mol H2/mol C), MCE was an important process, generating medium‐chain carboxylates up to octanoate (C8, 9.1 ± 1.3 mM C and 28.1 ± 4.1 mmol C m−2 d−1). Conversely, products with two carbons were the only ones present when the H2:IC ratio was low (<2.0 mol H2/mol C), so that H2 was the limiting factor. The biofilm microbial community was enriched in phylotypes most similar to the well‐known acetogen Acetobacterium for all conditions tested, but phylotypes closely related with families capable of MCE (e.g., Bacteroidales, Rhodocyclaceae, Alcaligenaceae, Thermoanaerobacteriales, and Erysipelotrichaceae) became important when the H2:IC ratio was high. Thus, proper management of IC availability and H2 supply allowed control over community structure and function, reflected by the chain length of the carboxylates and alcohols produced in the MBfR.

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Tài liệu tham khảo

10.1039/c2ee22101b

10.1016/j.tibtech.2010.11.006

10.1016/j.biortech.2012.05.003

10.1016/j.cej.2018.05.003

10.1099/00207713-27-4-355

10.1038/s41587-019-0209-9

10.1016/j.copbio.2021.01.005

Caporaso J. G., 2012, Ultra‐high‐throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms, The ISME Journal: Multidisciplinary Journal of Microbial Ecology, 6, 1621, 10.1038/ismej.2012.8

10.1073/pnas.1000080107

10.1016/j.cej.2016.08.049

10.1021/acs.est.5b06021

10.1186/1475-2859-11-128

10.1196/annals.1419.016

10.1186/s13068-017-0910-1

10.1016/j.biortech.2019.02.018

10.1016/j.biortech.2009.12.028

10.1016/j.biortech.2012.10.165

10.1016/j.biortech.2013.02.114

10.1016/j.biombioe.2012.11.019

10.1128/AEM.01614-18

10.1016/j.copbio.2007.03.008

Joshi S., 2021, The occurrence and ecology of microbial chain elongation of carboxylates in soils, The ISME Journal: Multidisciplinary Journal of Microbial Ecology

10.1039/C6EE01487A

10.1016/j.watres.2015.10.042

10.1016/j.copbio.2013.12.001

10.1007/s10967-015-4427-4

10.1016/j.biortech.2013.10.059

10.1016/j.biortech.2011.10.054

10.1016/j.biortech.2012.02.110

10.3390/fermentation3020028

10.1016/j.watres.2017.12.043

Rittmann B. E., 2020, Environmental biotechnology: Principles and applications

10.5194/acp-15-4399-2015

10.1016/j.jclepro.2018.08.162

10.1016/j.bej.2014.01.010

10.1016/j.biombioe.2013.01.034

10.1021/acs.est.8b03856

10.1016/j.copbio.2014.01.003

10.1016/j.watres.2008.05.032

10.1039/C0EE00282H

10.1016/j.memsci.2012.03.040

10.1016/j.biortech.2018.07.098

10.1016/j.jclepro.2018.03.193

10.1007/s00253-017-8124-9

10.1016/j.biortech.2017.10.054

10.1039/C5CC01897H

10.1016/j.biortech.2014.07.026

10.1007/s00253-013-5281-3

10.1016/j.watres.2013.07.033

10.1016/j.watres.2014.07.013

10.1002/bit.24487

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Biotechnology and Bioengineering

Tập 118 Số 6

2338-2347

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