Carbon isotope mass spectrometry in doping control
Tóm tắt
A procedure that makes it possible to establish the origin of endogenous steroids present in human urine by the determination of the 13C/12C carbon isotope ratio is described. A combination of solid-phase and liquid-liquid extraction, as well as semipreparative liquid chromatography, was used to separate fractions containing testosterone, 5α- and 5β-androstane-3α, 17β-diols, androsterone, ethiocholanolone, 5β-pregnane-3α, 20S-diol, and 16(5α)-androstene-3α-ol. More than 100 samples were analyzed by gas chromatography coupled with isotope mass spectrometry. The resulting values of 13C/12C were statistically processed, and the ranges required for the interpretation of the results were found. The procedure was certified and accredited in accordance with ISO 17025 requirements.
Tài liệu tham khảo
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