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Bổ sung carbon và độ sâu của đất ảnh hưởng đến sự phân bổ và phong phú của các vi khuẩn khử nitơ trong đất nông nghiệp
Tóm tắt
Các gen enzym khử nitrit (nirS và nirK) và gen mã hóa enzym khử oxit nitrous (nosZ) của các quần thể khử nitơ có trong đất đồng cỏ nông nghiệp đã được định lượng bằng phương pháp phản ứng chuỗi polymerase (PCR) theo thời gian thực. Các mẫu được lấy từ ba độ sâu đất khác nhau tại địa điểm đã chọn: tầng A (0–10 cm), tầng B (45–55 cm) và tầng C (120–130 cm). Tác động của việc bổ sung carbon (điều trị 1, đối chứng; điều trị 2, glucose-C; điều trị 3, carbon hữu cơ hòa tan (DOC)) đối với sự phong phú của gen khử nitơ và các lưu lượng N2O và N2 đã được xác định. Nói chung, sự phong phú của vi khuẩn khử nitơ có mối tương quan tốt với các phép đo lưu lượng; nirS có mối tương quan dương với N2O, và nosZ có mối tương quan dương với N2 (P < 0.03). Nồng độ bản sao gen khử nitơ trên mỗi gram đất (GCC) thay đổi tùy thuộc vào loại bổ sung carbon (P < 0.01). GCC của vi khuẩn khử nitơ cao (khoảng 10^7) và tỷ lệ bac:nirK, bac:nirS, bac:nirT và bac:nosZ đều thấp (khoảng 10−1/10) ở tầng A trong cả ba điều trị tương ứng. Việc bổ sung glucose-C đã thúc đẩy quá trình khử nitơ một phần, dẫn đến sự phong phú cao của gen nir và lưu lượng N2O tăng lên so với nhóm đối chứng. Ngược lại, việc bổ sung DOC đã dẫn đến sự phong phú nosZ tương đối cao và phát thải N2, do đó, thúc đẩy quá trình khử nitơ hoàn toàn. Chúng tôi cũng nhận thấy rằng độ sâu của đất ảnh hưởng trực tiếp đến sự phong phú của vi khuẩn, vi sinh vật cổ và vi sinh vật khử nitơ, có thể do sự thay đổi trong sự sẵn có của carbon trong đất theo độ sâu.
Từ khóa
#khử nitơ #nitrit #oxit nitrous #đất nông nghiệp #bổ sung carbon #PCRTài liệu tham khảo
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