Cadmium, chromium and copper in greengram plants
Tóm tắt
Soils contaminated with heavy metals including cadmium, chromium and copper present a major concern for sustainable agriculture. We studied the effects of cadmium, chromium and copper used both separately and as mixtures, on plant growth, nodulation, leghaemoglobin, seed yield and grain protein in seeds, in greengram inoculated with Bradyrhizobium sp. (Vigna). Cadmium at 24 mg kg−1 of soil reduced the dry matter accumulation and number of nodules by 27 and 38%, respectively. Chromium at 136 mg kg−1 of soil increased the dry phytomass and nodule numbers by 133 and 100%, respectively. The average maximum increase of 74% in seed yield occurred at 136 mg Cr kg−1 of soil. Cadmium and copper at 24 and 1338 mg kg−1 soil decreased the seed yield by 40 and 26%, respectively. Chromium at 136 kg−1 of soil increased the root and shoot N and leghaemoglobin content by 42, 31% and 50%, respectively. In contrast, the root and shoot N decreased by 22% at 24 mg Cd kg−1 of soil, while a maximum decrease of 50% in leghaemoglobin content occurred at 12 and 669 and 24 and 1338 mg Cd with Cu kg−1 of soil, relative to the control. The average maximum grain protein (283 mg g−1) was observed at 136 mg Cr kg−1 of soil, while minimum grain protein (231 mg g−1) was recorded at 24 and 1338 mg kg−1 of cadmium with copper. The metal accumulation in roots and shoots at 50 days after sowing and in grains 80 days after seeding differed among treatments. The degree of toxicity of heavy metals to the measured parameters decreased in the order Cd > Cu > Cr.
Tài liệu tham khảo
Asada K. (1994) Production and action of active oxygen species in photosynthetic tissues, in: Foyer C.H., Mullineaux P.M. (Eds.), Causes of photooxidative stress and amelioration of defense systems in plants, CRC Press, Boca Raton, pp. 77–104.
Assche F. Van, Clijsters H. (1990) Effects of metals on enzyme activity in plants, Plant Cell Environ. 13, 195–206.
Berry W.L., Wallace A. (1981) Toxicity: the concept and relationship to the dose response curve, J. Plant Nutr. 3, 13–19.
Bewley R.L.F., Stotzky G. (1983) Effects of cadmium and zinc on microbial activity in soil; Influence of clay minerals, Part II, metal added simultaneously, Sci. Total Environ. 31, 57–59.
Bibi M., Hussain M. (2005) Effect of copper and lead on photosynthesis and plant pigments in black gram (Vigna mungo L.), B. Environ. Contam. Tox. 74, 1126–1133.
Bisessar S., Rinne R.J., Potter J.W. (1983) Effect of heavy metals and Meloidogyne hapla on celery grown on organic soil near nickel refinery, Plant Dis. 67, 11–14.
Breen A.P., Murphy J.A. (1995) Reaction of oxyl radicals with DNA, Free Radical Bio. Med. 18, 1033–1077.
Broos K., Uyttebroek M., Mertens J., Smolders E. (2004) A survey of symbiotic nitrogen fixation by white clover grown on metal contaminated soils, Soil Biol. Biochem. 36, 633–640.
Broos K., Beyens H., Smolders E. (2005) Survival of rhizobia in soil is sensitive to elevated zinc in the absence of the host plant, Soil Biol. Biochem. 37, 573–579.
Chaoui A., Mazhoudi S., Ghorbal M.H., Ferjani E.E. (1997) Cadmium and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in bean (Phaeolus vulgaris L.), Plant Sci. 127, 139–147.
Charlier H.A. Jr, Albertson C., Thornock C., Warner L., Hurst T., Ellis R. (2005) Comparison of the effects of arsenic (V), cadmium (11), and mercury (11) single metal and mixed metal exposure in radish (Raphanus sativus), fescue grass (Festuca ovina) and duckweed (Lemna minor), B. Environ. Contam. Tox. 75, 474–481.
Chaudri A.M., McGrath S.P., Giller K.E., Reitz E., Suerbeck D.R. (1993) Enumeration of indigenous Rhizobium leguminosarum biovar trifolii in soils previously treated with metal contaminated sewage sludge, Soil Biol. Biochem. 25, 301–309.
Chaudri A.M., Allain C.M., Barbosa-Jefferson V.L., Nicholson F.A., Chambers B.J., McGrath S.P. (2000) A study of the impacts of Zn and Cu on two rhizobial species in soils of a long term field experiment, Plant Soil 22, 167–179.
Faisal M., Hasnain S. (2005) Bacterial Cr (VI) reduction concurrently improves sunflower (Helianthus annuus L.) growth, Biotechnol. Lett. 27, 943–947.
Gekeler W., Grill W.E., Winnacker E.-L., Zenk M.H. (1989) Survey of the plant kingdom for the ability to bind heavy metals through phytochelatins, Z. Naturforsch. 44c, 361–369.
Grill E., Winnacker E.L., Zenk M.H. (1985) Phytochelatins, the principal heavy metal complexing peptides of higher plants, Science 230, 674–676.
Grill E., Winnacker E.L., Zenk M.H. (1987) Phytochelatins, a class of heavy metal binding peptides from plants are functionally analogous to metallothioneins, Proc. Natl Acad. Sci. (USA) 84, 439–443.
Grill E., Loffler S., Winnacker E.L., Zenk M.H. (1989) Phytochelatins, the heavy metal binding peptides of plants are synthesized from glutathione by a specific U-glutamyl cysteine dipeptidyl transpeptidase (phytochelatin synthase), Proc. Natl Acad. Sci. (USA) 86 6838–6842.
Heckman J.R., Angle J.S., Chaney R.X. (1987) Residual effects of sewage sludge on soybean, II Accumulation of soil and symbiotically fixed nitrogen, J. Environ. Qual. 16, 117–124.
Hirsch P.R., Jones M.J., McGrath S.P., Giller K.E. (1993) Heavy metals from past applications of sewage sludge decrease the genetic diversity of Rhizobium leguminosarum biovar trifolii populations, Soil Biol. Biochem. 25, 1485–1490.
Ibekwe A.M., Angle J.S., Chaney R.L., Van Berkum P. (1995) Sewage sludge and heavy metal effects on nodulation and nitrogen fixation of legumes, J. Environ. Qual. 24, 1199–1204.
Ibekwe A.M., Angle J.S., Chaney R.L., Van Berkum P. (1996) Zinc and cadmium toxicity to alfalfa and its microsymbiont, J. Environ. Qual. 25, 1032–1040.
Iswaran V., Marwah T.S. (1980) A modified rapid Kjeldahl method for determination of total nitrogen in agricultural and biological materials, Geobios 7, 281–282.
Jackson P.J., Unkefer P.J., Delhaize E., Robinson N.J. (1990) Mechanisms of trace metal tolerance in plants, in: Katterman F. (Ed.), Environmental injury to plants, Academic Press, San Diego, pp. 231–258.
Lowrey O.H., Rosebrough N.J., Farr A.L., Randal R.J. (1951) Protein measurements with the Folin Phenol reagent, J. Biol. Chem. 193, 265–275.
Maitani T., Kubota H., Sato K., Yamada T. (1996) The composition of metals bound to class II metallothionein (phytochelatin and its desglycyl peptide) induced by various metals in root cultures of Rubia tinctorum, Plant Physiol. 110, 1145–1150.
McCarthy I., Romero-Puertas M.C., Palma J.M., Sandalio L.M., Corpas F.J., Gomez M., del Rio L.A. (2001) Cadmium induces senescence systems in leaf peroxisomes of pea plants, Plant Cell Environ. 24, 1065–1073.
McGrath S.P., Brookes P.C., Giller K.E. (1988) Effect of potentially toxic metals in soil derived from past applications of sewage sludge on nitrogen fixation by Trifolium repens L., Soil Biol. Biochem. 20, 415–425.
McGrath S.P., Cunliffe C.H. (1985) A simplified method for the extraction of metals Fe, Zn, Cu, Ni, Cd, Pb, Cr and Mn from soils and sewage sludge, J. Sci. Food Agr. 36, 794–798.
Ouzounidou G.E., Eleftheriou P., Karatagli S. (1992) Ecophysiological and ultrastructural effects of copper in Thlaspi Ochroleucum (cruciferae), Can. J. Bot. 70, 947–957.
Prasad M.N.V. (1999) Heavy metal stress in plants from biomolecules to ecosystem, Narosa Publishing House, New Delhi, India.
Roane T.M., Kellogg S.T. (1996) Characterization of bacterial communities in heavy metal-contaminated soils, Can. J. Microbiol. 42, 593–603.
Rother J.A., Millbank J.W., Thornton I. (1983) Nitrogen fixation by white clover (Trifolium repens) in grasslands on soils contaminated with cadmium, lead and zinc, J. Soil Sci. 34, 127–136.
Sadasivam S., Manikam A. (1992) Biochemical methods for agricultural sciences, Wiley Eastern Limited, New Delhi, India.
Shainberg O., Rubin B., Rabinowitch H.D., Tel-Or E. (2001) Loading beans with sublethal levels of copper enhances conditioning to oxidative stress, J. Plant Physiol. 158, 1415–1421.
Sheoran I.S., Signal H.R., Singh R. (1990) Effect of Cd and Ni on photosynthesis and the enzymes of the photosynthetic carbon reduction cycle in pigeonpea (Cajans cajan L.), Photosynth. Res. 23, 345–351.
Singh C., Singh P., Singh R. (2004) Modern techniques of raising field crops, India, Oxford and IBH Publishing Company, Private Limited, pp. 209–218.
Sinha S.K., Srivastava H.S., Misra S.N. (1988) Nitrate assimilation in intact and excised maize leaves in the presence of lead, B. Environ. Contain. Tox. 41, 419–426.
Steffens J.C. (1990) The heavy metal binding peptides of plants, Ann. Rev. Plant Physiol. Plant Mol. Biol. 41, 553–575.
Stephen J.V., Weidensaul T.C. (1978) Effects of cadmium, nickel, copper and zinc on nitrogen fixation by soybeans, Water Air Soil Poll. 9, 416–422.
Wani P.A., Khan M.S., Zaidi A. (2006) An evaluation of the effects of heavy metals on the growth, seed yield and grain protein of lentil in pots, Ann. Appl. Biol. (Suppl. TAC) 27, 23–24.
Yadav O.P., Shukla U.C. (1983) Effect of zinc on nodulation and nitrogen fixation in chickpea (Cicer arietinum L.), J. Agr. Sci. 101, 559–563.