Vỏ quả cacao trong sản xuất axit citric dưới quá trình lên men rắn sử dụng phương pháp bề mặt phản hồi

Biomass Conversion and Biorefinery - Tập 13 - Trang 7165-7173 - 2021
Urip Perwitasari1, Nursita Tria Agustina2, Radityo Pangestu1, Siti Amanah1, Hendra Saputra1, Ade Andriani1, Fahrurrozi1, Ario Betha Juanssilfero1, Ahmad Thontowi1, Tri Dewanti Widyaningsih2, Deden Dewantara Eris3, Maghfirotul Amaniyah4, Yopi1,5, Muhammad Sidiq Habibi6
1Research Center for Biotechnology, Indonesian Institute of Sciences, Cibinong, Indonesia
2Department of Food Science and Technology, Faculty of Agricultural Technology, Brawijaya University, Malang, Indonesia
3Indonesian Research Institute for Biotechnology and Bioindustry, Bogor, Indonesia
4Study Program of Livestock Product Processing Technology, Politeknik Negeri Banyuwangi, Banyuwangi, Indonesia
5Research Center and Human Resource Development, Standardization Agency of Indonesia, Jakarta, Indonesia
6PT Andalan Furnindo, Kawasan Industri Dan Pergudangan, Bekasi, Indonesia

Tóm tắt

Indonesia là một trong những quốc gia sản xuất ca cao lớn nhất thế giới với tổng sản lượng đạt 240.000 tấn vào năm 2019. Trong khi đó, chúng ta có khoảng 400.000 tấn vỏ quả cacao. Biomass từ vỏ quả cacao là một vấn đề nghiêm trọng tại Indonesia, nhưng đồng thời, nó cũng là một nguồn tài nguyên tái tạo quan trọng, đầy thách thức và thân thiện với môi trường cho sản xuất sinh học. Do đó, nó trở thành một mục tiêu nghiên cứu trong nghiên cứu này nhằm mục đích tận dụng vỏ quả cacao như một chất nền tái tạo cho sản xuất axit citric. Phương pháp lên men thể rắn (SSF) đã được áp dụng cho vỏ quả cacao sử dụng nấm Aspergillus niger Tiegh F359 để sản xuất axit citric. Phương pháp bề mặt phản hồi (RSM) đã được sử dụng để tối ưu hóa quá trình sản xuất axit citric với nồng độ nước, nồng độ nitơ và thời gian lên men là các yếu tố biến đổi. Khoảng 7530 ppm axit citric đã được sản xuất từ 1 g vỏ quả cacao như một nguồn cacbon. Tóm lại, nghiên cứu này đã thành công trong việc tối ưu hóa sản xuất axit citric bằng cách sử dụng SSF với A. niger Tiegh F359 và cung cấp một nghiên cứu cơ bản cho vỏ quả cacao như một chất nền tái tạo chi phí thấp cho sản xuất sản phẩm sinh học và các nỗ lực trong tương lai.

Từ khóa

#vỏ quả cacao #axit citric #lên men thể rắn #phương pháp bề mặt phản hồi #sản xuất sinh học

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