CXCL12-induced glioblastoma cell migration requires intermediate conductance Ca<sup>2+</sup>-activated K<sup>+</sup> channel activity

American Journal of Physiology - Cell Physiology - Tập 299 Số 1 - Trang C175-C184 - 2010
Miriam Sciaccaluga1, Bernard Fioretti2, Luigi Catacuzzeno2, Francesca Pagani1, Cristina Bertollini1, Maria Rosito1, Myriam Catalano1, Giuseppina D’Alessandro1, Antonio Santoro3, Giampaolo Cantore4, Davide Ragozzino1, Emilia Castigli2, Fabio Franciolini2, Cristina Limatola1,4
1Istituto Pasteur-Fondazione Cenci Bolognetti and Department of Physiology and Pharmacology, and
2Department of Cellular and Environmental Biology, University of Perugia, Perugia; and
3Department of Neurological Science, Sapienza University of Rome, Rome
4Neuromed IRCCS, Via Atinese, Pozzilli, Italy

Tóm tắt

The activation of ion channels is crucial during cell movement, including glioblastoma cell invasion in the brain parenchyma. In this context, we describe for the first time the contribution of intermediate conductance Ca2+-activated K (IKCa) channel activity in the chemotactic response of human glioblastoma cell lines, primary cultures, and freshly dissociated tissues to CXC chemokine ligand 12 (CXCL12), a chemokine whose expression in glioblastoma has been correlated with its invasive capacity. We show that blockade of the IKCa channel with its specific inhibitor 1-[(2-chlorophenyl) diphenylmethyl]-1 H-pyrazole (TRAM-34) or IKCa channel silencing by short hairpin RNA (shRNA) completely abolished CXCL12-induced cell migration. We further demonstrate that this is not a general mechanism in glioblastoma cell migration since epidermal growth factor (EGF), which also activates IKCa channels in the glioblastoma-derived cell line GL15, stimulate cell chemotaxis even if the IKCa channels have been blocked or silenced. Furthermore, we demonstrate that both CXCL12 and EGF induce Ca2+ mobilization and IKCa channel activation but only CXCL12 induces a long-term upregulation of the IKCa channel activity. Furthermore, the Ca2+-chelating agent BAPTA-AM abolished the CXCL12-induced, but not the EGF-induced, glioblastoma cell chemotaxis. In addition, we demonstrate that the extracellular signal-regulated kinase (ERK)1/2 pathway is only partially implicated in the modulation of CXCL12-induced glioblastoma cell movement, whereas the phosphoinositol-3 kinase (PI3K) pathway is not involved. In contrast, EGF-induced glioblastoma migration requires both ERK1/2 and PI3K activity. All together these findings suggest that the efficacy of glioblastoma invasiveness might be related to an array of nonoverlapping mechanisms activated by different chemotactic agents.

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Thông tin xuất bản

American Journal of Physiology - Cell Physiology

Tập 299 Số 1

C175-C184

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