CRISPR-based knock-out of eIF4E2 in a cherry tomato background successfully recapitulates resistance to pepper veinal mottle virus

Veillet, 2020, Precision breeding made real with CRISPR: illustration through genetic resistance to pathogens, Plant Commun., 1, 10.1016/j.xplc.2020.100102 Bastet, 2017, eIF4E resistance: natural variation should guide gene editing, Trends Plant Sci., 22, 411, 10.1016/j.tplants.2017.01.008 Ruffel, 2002, A natural recessive resistance gene against potato virus Y in pepper corresponds to the eukaryotic initiation factor 4E (elF4E), Plant J., 32, 10.1046/j.1365-313X.2002.01499.x Charron, 2008, Natural variation and functional analyses provide evidence for co-evolution between plant eIF4E and potyviral VPg, Plant J., 54, 10.1111/j.1365-313X.2008.03407.x Robaglia, 2006, Translation initiation factors: a weak link in plant RNA virus infection, Trends Plant Sci., 11, 40, 10.1016/j.tplants.2005.11.004 Schmitt-Keichinger, 2019, Manipulating cellular factors to combat viruses: a case study from the plant eukaryotic translation initiation factors eIF4, Front. Microbiol., 10, 10.3389/fmicb.2019.00017 Jacob, 2018, Translational research: exploring and creating genetic diversity, Trends Plant Sci., 23, 10.1016/j.tplants.2017.10.002 Piron, 2010, An induced mutation in tomato eiF4E leads to immunity to two potyviruses, PLoS One, 5, 10.1371/journal.pone.0011313 Gauffier, 2016, A TILLING approach to generate broad-spectrum resistance to potyviruses in tomato is hampered by eIF4E gene redundancy, Plant J., 85, 717, 10.1111/tpj.13136 Lebaron, 2016, A new eIF4E1 allele characterized by RNAseq data mining is associated with resistance to potato virus Y in tomato albeit with a low durability, J. Gen. Virol., 97, 3063, 10.1099/jgv.0.000609 Rothan, 2019, Trait discovery and editing in tomato, Plant J., 97, 10.1111/tpj.14152 Ruffel, 2005, The recessive potyvirus resistance gene pot-1 is the tomato orthologue of the pepper pvr2-eIF4E gene, Mol. Genet. Genomics, 274, 10.1007/s00438-005-0003-x Mazier, 2011, Knock-down of both eIF4E1 and eIF4E2 genes confers broad-spectrum resistance against potyviruses in tomato, PLoS One, 6, 10.1371/journal.pone.0029595 Moury, 2020, Knock-out mutation of eukaryotic initiation factor 4E2 (eIF4E2) confers resistance to pepper veinal mottle virus in tomato, Virology, 539, 10.1016/j.virol.2019.09.015 Danilo, 2019, Efficient and transgene-free gene targeting using Agrobacterium-mediated delivery of the CRISPR/Cas9 system in tomato, Plant Cell Rep., 38, 10.1007/s00299-019-02373-6 Veillet, 2019, Transgene-free genome editing in tomato and potato plants using agrobacterium-mediated delivery of a CRISPR/Cas9 cytidine base editor, Int. J. Mol. Sci., 20, 402, 10.3390/ijms20020402 Lellis, 2002, Loss-of-susceptibility mutants of Arabidopsis thaliana reveal an essential role for elF(iso)4E during potyvirus infection, Curr. Biol., 12, 10.1016/S0960-9822(02)00898-9 Duprat, 2002, The Arabidopsis eukaryotic initiation factor (iso)4E is dispensable for plant growth but required for susceptibility to potyviruses, Plant J., 32, 10.1046/j.1365-313X.2002.01481.x Pyott, 2016, Engineering of CRISPR/Cas9-mediated potyvirus resistance in transgene-free Arabidopsis plants, Mol. Plant Pathol., 17, 1276, 10.1111/mpp.12417 Chandrasekaran, 2016, Development of broad virus resistance in non-transgenic cucumber using CRISPR/Cas9 technology, Mol. Plant Pathol., 17, 1140, 10.1111/mpp.12375 Gomez, 2019, Simultaneous CRISPR/Cas9-mediated editing of cassava eIF4E isoforms nCBP-1 and nCBP-2 reduces cassava brown streak disease symptom severity and incidence, Plant Biotechnol. J., 17, 10.1111/pbi.12987 Yoon, 2020, Genome editing of eIF4E1 in tomato confers resistance to pepper mottle virus, Front. Plant Sci., 11, 1098, 10.3389/fpls.2020.01098 Atarashi, 2020, Artificially edited alleles of the eukaryotic translation initiation factor 4E1 gene differentially reduce susceptibility to cucumber mosaic virus and potato virus Y in tomato, Front. Microbiol., 11, 3075, 10.3389/fmicb.2020.564310 Veillet, 2020, Expanding the CRISPR toolbox in P. Patens using SpCas9-NG variant and application for gene and base editing in solanaceae crops, Int. J. Mol. Sci., 21, 10.3390/ijms21031024 Fulton, 1995, Microprep protocol for extraction of DNA from tomato and other herbaceous plants, Plant Mol. Biol. Report, 13, 10.1007/BF02670897 Veillet, 2016, Targeting the AtCWIN1 gene to explore the role of invertases in sucrose transport in roots and during Botrytis cinerea infection, Front. Plant Sci., 7, 10.3389/fpls.2016.01899 Parrella, 2002, Recessive resistance genes against potyviruses are localized in colinear genomic regions of the tomato (Lycopersicon spp.) and pepper (Capsicum spp.) genomes, Theor. Appl. Genet., 105, 10.1007/s00122-002-1005-2 Moury, 2004, Mutations in Potato virus Y genome-linked protein determine virulence toward recessive resistances in Capsicum annuum and Lycopersicon hirsutum, Mol. Plant Microbe Interact., 17, 10.1094/MPMI.2004.17.3.322 Nishimasu, 2018, Engineered CRISPR-Cas9 nuclease with expanded targeting space, Science (80-.), 361, 1259, 10.1126/science.aas9129 Monzingo, 2007, The structure of eukaryotic translation initiation factor-4E from wheat reveals a novel disulfide bond, Plant Physiol., 143, 10.1104/pp.106.093146 Miras, 2017, Structure of eiF4E in complex with an eiF4G peptide supports a universal bipartite binding mode for protein translation, Plant Physiol., 174, 10.1104/pp.17.00193 Ashby, 2011, Structure-based mutational analysis of eIF4E in relation to sbm1 resistance to Pea seed-borne mosaic virus in Pea, PLoS One, 6, 10.1371/journal.pone.0015873 Levins, 2016, Fusion proteins of Arabidopsis cap-binding proteins: cautionary “tails” of woe, Translation, 4, 10.1080/21690731.2016.1257408 Ranc, 2008, A clarified position for solanum lycopersicum var. cerasiforme in the evolutionary history of tomatoes (solanaceae), BMC Plant Biol., 8, 10.1186/1471-2229-8-130 Gallois, 2018, Role of the genetic background in resistance to plant viruses, Int. J. Mol. Sci., 19, 10.3390/ijms19102856 Bastet, 2018, Trans-species synthetic gene design allows resistance pyramiding and broad-spectrum engineering of virus resistance in plants, Plant Biotechnol. J., 16, 1569, 10.1111/pbi.12896 Zafirov, 2021, When a knockout is an Achilles’ heel: Resistance to one potyvirus species triggers hypersusceptibility to another one in Arabidopsis thaliana, Mol. Plant Pathol., 22, 334, 10.1111/mpp.13031 Callot, 2014, Pyramiding resistances based on translation initiation factors in Arabidopsis is impaired by male gametophyte lethality, Plant Signal. Behav., 9, 10.4161/psb.27940 Patrick, 2014, Two arabidopsis loci encode novel eukaryotic initiation factor 4E isoforms that are functionally distinct from the conserved plant eukaryotic initiation factor 4E, Plant Physiol., 164, 10.1104/pp.113.227785 Bastet, 2019, Mimicking natural polymorphism in eIF4E by CRISPR-Cas9 base editing is associated with resistance to potyviruses, Plant Biotechnol. J., 17, 1736, 10.1111/pbi.13096 van Schie, 2014, Susceptibility genes 101: how to be a good host, Annu. Rev. Phytopathol., 52, 551, 10.1146/annurev-phyto-102313-045854