CHO cell engineering to prevent polypeptide aggregation and improve therapeutic protein secretion

Aggarwal, 2011, What is fueling the biotech engine—2010 to 2011, Nat. Biotechnol., 29, 1083, 10.1038/nbt.2060 Baik, 2012, Metabolic engineering of Chinese hamster ovary cells: towards a bioengineered heparin, Metab. Eng., 14, 81, 10.1016/j.ymben.2012.01.008 Borth, 2005, Effect of increased expression of protein disulfide isomerase and heavy chain binding protein on antibody secretion in a recombinant CHO cell line, Biotechnol. Progr., 21, 106, 10.1021/bp0498241 Bovia, 1995, The SRP9/14 subunit of the signal recognition particle (SRP) is present in more than 20-fold excess over SRP in primate cells and exists primarily free but also in complex with small cytoplasmic Alu RNAs, Mol. Biol. Cell, 66, 471, 10.1091/mbc.6.4.471 Chang, 1995, A trinucleotide repeat-associated increase in the level of Alu RNA-binding protein occurred during the same period as the major Alu amplification that accompanied anthropoid evolution, Mol. Cell. Biol., 15, 2109, 10.1128/MCB.15.4.2109 Chung, 2004, Effect of doxycycline-regulated calnexin and calreticulin expression on specific thrombopoietin productivity of recombinant Chinese hamster ovary cells, Biotechnol. Bioeng., 85, 539, 10.1002/bit.10919 Davis, 2000, Effect of PDI overexpression on recombinant protein secretion in CHO cells, Biotechnol. Progr., 16, 736, 10.1021/bp000107q Gilmore, 1982, Protein translocation across the endoplasmic reticulum. I. Detection in the microsomal membrane of a receptor for the signal recognition particle, J. Cell Biol., 95, 463, 10.1083/jcb.95.2.463 Girod, 2007, Genome-wide prediction of matrix attachment regions that increase gene expression in mammalian cells, Nat. Methods, 4, 747, 10.1038/nmeth1076 Goh, 2010, RCA-I-resistant CHO mutant cells have dysfunctional GnT I and expression of normal GnT I in these mutants enhances sialylation of recombinant erythropoietin, Metab. Eng., 12, 360, 10.1016/j.ymben.2010.03.002 Hwang, 2003, Effect of doxycycline-regulated ERp57 expression on specific thrombopoietin productivity of recombinant CHO cells, Biotechnol. Progr., 19, 179, 10.1021/bp025578m Johansson, 1993, Positively charged amino acids placed next to a signal sequence block protein translocation more efficiently in Escherichia coli than in mammalian microsomes, Mol. Genomics Genet., 239, 251, 10.1007/BF00281625 Kang, 2006, Substrate-specific translocational attenuation during ER stress defines a pre-emptive quality control pathway, Cell, 127, 999, 10.1016/j.cell.2006.10.032 Keenan, 2001, The signal recognition particle, Annu. Rev. Biochem., 70, 755, 10.1146/annurev.biochem.70.1.755 Khan, 2008, Engineering of chaperone systems and of the unfolded protein response, Cytotechnology, 57, 207, 10.1007/s10616-008-9157-9 Lakkaraju, 2008, SRP keeps polypeptides translocation-competent by slowing translation to match limiting ER-targeting sites, Cell, 133, 440, 10.1016/j.cell.2008.02.049 Le Fourn, 2009, Basal autophagy is involved in the degradation of the ERAD component EDEM1, Cell. Mol. Life Sci., 66, 1434, 10.1007/s00018-009-9038-1 Lim, 2010, Engineering mammalian cells in bioprocessing—current achievements and future perspectives, Biotechnol. Appl. Biochem., 55, 175, 10.1042/BA20090363 Mariati, 2010, Evaluating post-transcriptional regulatory elements for enhancing transient gene expression levels in CHO K1 and HEK293 cells, Protein Expression Purif., 69, 9, 10.1016/j.pep.2009.08.010 Mariati, 2012, Post-transcriptional regulatory elements for enhancing transient gene expression levels in mammalian cells, Methods Mol. Biol., 801, 125, 10.1007/978-1-61779-352-3_9 Melnick, 1992, The endoplasmic reticulum stress protein GRP94, in addition to BiP, associates with unassembled immunoglobulin chains, J. Biol. Chem., 267, 21303, 10.1016/S0021-9258(19)36608-6 Mohan, 2010, Effect of inducible co-overexpression of protein disulfide isomerase and endoplasmic reticulum oxidoreductase on the specific antibody productivity of recombinant Chinese hamster ovary cells, Biotechnol. Bioeng., 107, 337, 10.1002/bit.22781 Morris, 1997, Immunoglobulin binding protein (BiP) function is required to protect cells from endoplasmic reticulum stress but is not required for the secretion of selective proteins, J. Biol. Chem., 272, 4327, 10.1074/jbc.272.7.4327 Nehlsen, 2009, Recombinant protein expression by targeting pre-selected chromosomal loci, BMC Biotech., 9, 100, 10.1186/1472-6750-9-100 Peng, 2009, Molecular engineering of exocytic vesicle traffic enhances the productivity of Chinese hamster ovary cells, Biotechnol. Bioeng., 102, 1170, 10.1002/bit.22141 Peng, 2010, The vesicle-trafficking protein munc18b increases the secretory capacity of mammalian cells, Metab. Eng., 12, 18, 10.1016/j.ymben.2009.08.007 Rutkowski, 2008, UPR pathways combine to prevent hepatic steatosis caused by ER stress-mediated suppression of transcriptional master regulators, Dev. Cell, 15, 829, 10.1016/j.devcel.2008.10.015 Tan, 2008, Overexpression of cold-inducible RNA-binding protein increases interferon-gamma production in Chinese-hamster ovary cells, Biotechnol. Appl. Biochem., 49, 247, 10.1042/BA20070032 Tigges, 2006, Xbp1-based engineering of secretory capacity enhances the productivity of Chinese hamster ovary cells, Metab. Eng., 8, 264, 10.1016/j.ymben.2006.01.006 Walter, 1981, Translocation of proteins across the endoplasmic reticulum III. Signal recognition protein (SRP) causes signal sequence-dependent and site-specific arrest of chain elongation that is released by microsomal membranes, J. Cell Biol., 91, 557, 10.1083/jcb.91.2.557 Walter, 1982, The protein translocation machinery of the endoplasmic reticulum, Philos. Trans. R. Soc. London, Ser. B, 300, 225, 10.1098/rstb.1982.0168 Zhang, 2010, A functional analysis of N-glycosylation-related genes on sialylation of recombinant erythropoietin in six commonly used mammalian cell lines, Metab. Eng., 12, 526, 10.1016/j.ymben.2010.08.004