CD133, a novel marker for human prostatic epithelial stem cells

Journal of Cell Science - Tập 117 Số 16 - Trang 3539-3545 - 2004
Gavin D. Richardson1, Craig Robson1, Shona Lang2, David E. Neal3, Norman J. Maitland2, Anne T. Collins2
1Prostate Research Group, Surgical Oncology, Medical School, Framlington Place, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, UK
2YCR Cancer Research Unit (Area 13), Department of Biology, University of York, PO BOX 373, York, YO10 5DD, UK
3Department of Oncology, Addenbrooke's Hospital, University of Cambridge, Hills Road, Cambridge, CB2 2QQ, UK

Tóm tắt

Stem cells are clonogenic cells with self-renewal and differentiation properties, which may represent a major target for genetic damage leading to prostate cancer and benign prostatic hyperplasia. Stem cells remain poorly characterised because of the absence of specific molecular markers that permit us to distinguish them from their progeny, the transit amplifying cells, which have a more restricted proliferative potential. Human CD133 antigen, also known as AC133, was recently identified as a haematopoietic stem cell marker. Here we show that a small population (approximately 1%) of human prostate basal cells express the cell surface marker CD133 and are restricted to the α2β1hi population, previously shown to be a marker of stem cells in prostate epithelia (Collins, A. T., Habib, F. K., Maitland, N. J. and Neal, D. E. (2001). J. Cell Sci. 114, 3865-3872). α2β1hi/CD133+ cells exhibit two important attributes of epithelial stem cells: they possess a high in vitro proliferative potential and can reconstitute prostatic-like acini in immunocompromised male nude mice.

Từ khóa


Tài liệu tham khảo

Collins, A. T., Habib, F. K., Maitland, N. J. and Neal, D. E. (2001). Identification and isolation of human prostate epithelial stem cells based on alpha(2)beta(1)-integrin expression. J. Cell Sci.114, 3865-3872.

Corbeil, D., Roper, K., Hellwig, A., Tavian, M., Miraglia, S., Watt, S. M., Simmons, P. J., Peault, B., Buck, D. W. and Huttner, W. B. (2000). The human AC133 hematopoietic stem cell antigen is also expressed in epithelial cells and targeted to plasma membrane protrusions. J. Biol. Chem.275, 5512-5520.

Coucouvanis, E. and Martin, G. R. (1995). Signals for death and survival, a two-step mechanism for cavitation in the vertebrate embryo. Cell83, 279-287.

Cunha, G. R. and Lung, B. (1978). The possible influence of temporal factors in androgenic responsiveness of urogenital tissue recombinants from wild-type and androgen-insensitive (Tfm) mice. J. Exp. Zool.205, 181-193.

Cunha, G. R., Fujii, H., Neubauer, B. L., Shannon, J. M., Sawyer, L. and Reese, B. A. (1983). Epithelial-mesenchymal interactions in prostatic development. I. morphological observations of prostatic induction by urogenital sinus mesenchyme in epithelium of the adult rodent urinary bladder. J. Cell Biol.96, 1662-1670.

Cunha, G. R., Donjacour, A. A., Cooke, P. S., Mee, S., Bigsby, R. M., Higgins, S. J. and Sugimura, Y. (1987). The endocrinology and developmental biology of the prostate. Endocr. Rev.8, 338-362.

Demarchez, M., Hartmann, D. J., Regnier, M. and Asselineau, D. (1992). The role of fibroblasts in dermal vascularization and remodeling of reconstructed human skin after transplantation onto the nude mouse. Transplantation54, 317-326.

Donjacour, A. A. and Cunha, G. R. (1993). Assessment of prostatic protein secretion in tissue recombinants made of urogenital sinus mesenchyme and urothelium from normal or androgen-insensitive mice. Endocrinology132, 2342-2350.

Frisch, S. M. and Francis, H. (1994). Disruption of epithelial cell-matrix interactions induces apoptosis. J. Cell Biol.124, 619-626.

Hudson, D. L., Guy, A. T., Fry, P., O'Hare, M. J., Watt, F. M. and Masters, J. R. (2001). Epithelial cell differentiation pathways in the human prostate, identification of intermediate phenotypes by keratin expression. J. Histochem. Cytochem.49, 271-278.

Isaacs, J. T. and Coffey, D. S. (1989). Etiology and disease process of benign prostatic hyperplasia. Prostate Suppl.2, 33-50.

Isaacs, J. T., Schulze, H. and Coffey, D. S. (1987). Development of androgen resistance in prostatic cancer. Prog. Clin. Biol. Res.243, 21-31.

Jones, J., Downer, C. S. and Speight, P. M. (1995). Changes in the expression of integrins and basement membrane proteins in benign mucous membrane pemphigoid. Oral Dis.1, 159-165.

Lang, S. H., Stark, M., Collins, A., Paul, A. B., Stower, M. J. and Maitland, N. J. (2001). Experimental prostate epithelial morphogenesis in response to stroma and three-dimensional matrigel culture. Cell Growth Differ.12, 631-640.

Marley, S. B., Lewis, J. L., Davidson, R. J., Roberts, I. A., Dokal, I., Goldman, J. M. and Gordon, M. Y. (1999). Evidence for a continuous decline in haemopoietic cell function from birth, application to evaluating bone marrow failure in children. Br. J. Haematol.106, 162-166.

Michel, M., Torok, N., Godbout, M. J., Lussier, M., Gaudreau, P., Royal, A. and Germain, L. (1996). Keratin 19 as a biochemical marker of skin stem cells in vivo and in vitro, keratin 19 expressing cells are differentially localized in function of anatomic sites, and their number varies with donor age and culture stage. J. Cell Sci.109, 1017-1028.

Morrison, S. J. and Weissman, I. L. (1994). The long-term repopulating subset of hematopoietic stem cells is deterministic and isolatable by phenotype. Immunity1, 661-673.

Morrison, J., Shah, N. M. and Anderson, D. J. (1997). Regulatory mechanisms in stem cell biology. Cell88, 287-298.

Peichev, M., Naiyer, A. J., Pereira, D., Zhu, Z., Lane, W. J., Williams, M., Oz, M. C., Hicklin, D. J., Witte, L., Moore, M. A. et al. (2000). Expression of VEGFR-2 and AC133 by circulating human CD34(+) cells identifies a population of functional endothelial precursors. Blood95, 952-958.

Pierce, G. B. and Wallace, C. (1971). Differentiation of malignant to benign cells. Cancer Res.31, 127-134.

Pullan, S., Wilson, J., Metcalfe, A., Edwards, G. M., Goberdhan, N., Tilly, J., Hickman, J. A., Dive, C. and Streuli, C. H. (1996). Requirement of basement membrane for the suppression of programmed cell death in mammary epithelium. J. Cell Sci.109, 631-642.

Raff, M. C., Barres, B. A., Burne, J. F., Coles, H. S., Ishizaki, Y. and Jacobson, M. D. (1993). Programmed cell death and the control of cell survival, lessons from the nervous system. Science262, 695-700.

Reya, T., Morrison, S. J., Clarke, M. F. and Weissman, I. L. (2001). Stem cells, cancer, and cancer stem cells. Nature414, 105-111.

Robinson, E. J., Neal, D. E. and Collins, A. T. (1998). Basal cells are progenitors of luminal cells in primary cultures of differentiating human prostatic epithelium. Prostate37, 149-160.

Sherwood, E. R., Berg, L. A., Mitchell, N. J., McNeal, J. E., Kozlowski, J. M. and Lee, C. (1990). Differential cytokeratin expression in normal, hyperplastic and malignant epithelial cells from human prostate. J. Urol.143, 167-171.

Sherwood, E. R., Theyer, G., Steiner, G., Berg, L. A., Kozlowski, J. M. and Lee, C. (1991). Differential expression of specific cytokeratin polypeptides in the basal and luminal epithelia of the human prostate. Prostate18, 303-314.

Spangrude, G. J., Heimfeld, S. and Weissman, I. L. (1988). Purification and characterization of mouse hematopoietic stem cells. Science241, 58-62.

Tsujimura, A., Koikawa, Y., Salm, S., Takao, T., Coetzee, S., Moscatelli, D., Shapiro, E., Lepor, H., Sun, T. T. and Wilson, L. (2002). Proximal location of mouse prostate epithelial stem cells: a model of prostatic homeostasis. J. Cell Biol.157, 1257-1265.

Uchida, N., Buck, D. W., He, D., Reitsma, M. J., Masek, M., Phan, T. V., Tsukamoto, A. S., Gage, F. H. and Weissman, I. L. (2000). Direct isolation of human central nervous system stem cells. Proc. Natl. Acad. Sci. USA97, 14720-14725.

van Leenders, G., Dijkman, H., Hulsbergen-van de Kaa, C., Ruiter, D. and Schalken, J. (2000). Demonstration of intermediate cells during human prostate epithelial differentiation in situ and in vitro using triple-staining confocal scanning microscopy. Lab. Invest.80, 1251-1258.

Weigmann, A., Corbeil, D., Hellwig, A. and Huttner, W. B. (1997). Prominin, a novel microvilli-specific polytopic membrane protein of the apical surface of epithelial cells, is targeted to plasmalemmal protrusions of non-epithelial cells. Proc. Natl. Acad. Sci. USA94, 12425-12430.

Yin, A. H., Miraglia, S., Zanjani, E. D., Almeida-Porada, G., Ogawa, M., Leary, A. G., Olweus, J., Kearney, J. and Buck, D. W. (1997). AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood90, 5002-5012.

Zhang, Z., Vuori, K., Reed, J. C. and Ruoslahti, E. (1995). The alpha 5 beta 1 integrin supports survival of cells on fibronectin and up-regulates Bcl-2 expression. Proc. Natl. Acad. Sci. USA92, 6161-6165.

Thông tin
Thông tin xuất bản

Journal of Cell Science

Tập 117 Số 16

3539-3545

Thông tin tác giả