C-dots assisted synthesis of gold nanoparticles as labels to catalyze copper deposition for ultrasensitive electrochemical sensing of proteins
Tóm tắt
We report an ultrasensitive protocol for electrochemical sensing using the hydroxyl-rich C-dots assisted synthesis of gold nanoparticles (C-dots@AuNP) as labels with copper depositon reaction. The C-dots catalyzing copper deposition reaction was implemented for the first time. We constructed a sandwich-type immunosensor on the chitosan modified glassy carbon electrode (GCE) by glutaraldehyde (GA) crosslinking, with C-dots@AuNP as biolabels. Copper was deposited on the catalytic surfaces of second antibody-conjugated C-dots@AuNP nanoparticles through CuSO4-ascorbic acid reduction, because both C-dots and AuNPs could strongly catalyze the CuSO4 and ascorbic acid to form Cu particles, which amplified the detection signal. Then the corresponding antigen was quantified based on simultaneous chemical-dissolution/cathodic-preconcentration of copper for in-situ analysis using anodic stripping square wave voltammetry (ASSWV) directly on the modified electrode. Under optimized conditions, these electrodes were employed for sandwich-type immunoanalysis, pushing the lower limits of detection (LODs) down to the fg mL−1 level for human immunoglobulin G (IgG) and cardiac troponin I (cTnI), a cardiac biomarker. These novel sensors have good stability and acceptable accuracy and reproducibility, suggesting potential applications in clinical diagnostics.
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