Bone marrow concentrate and platelet-rich plasma acquisition and preparation: Why technique matters

Murphy, 2010, Engineering a better way to heal broken bones, Chem Eng Prog, 106, 37 Buchanan, 2014, Stem cell therapy for regenerative medicine, Chem Eng Prog, 110, 55 Cohnheim, 1867, Ueber Entzu ¨ndung und Eiterung, Arch fu ¨r Pathol Anat und Physiol und fu ¨r Klin Med, 40, 1, 10.1007/BF02968135 Friedenstein, 1974, Stromal cells responsible for transferring the microenvironment of the hemopoietic tissues. Cloning in vitro and retransplantation in vivo, Transplantation, 17, 331, 10.1097/00007890-197404000-00001 Civin, 1984, Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells, J Immunol, 133, 157, 10.4049/jimmunol.133.1.157 Tindle, 1985, A novel monoclonal antibody BI-3C5 recognises myeloblasts and non-B non-T lymphoblasts in acute leukaemias and CGL blast crises, and reacts with immature cells in normal bone marrow, Leuk Res, 9, 1, 10.1016/0145-2126(85)90016-5 Thomas, 1975, Bone-marrow transplantation, N Engl J Med, 292, 895, 10.1056/NEJM197504242921706 Murphy, 2013, Mesenchymal stem cells: environmentally responsive therapeutics for regenerative medicine, Exp Mol Med, 45, e54, 10.1038/emm.2013.94 Chargaff, 1936, Studies on the chemistry of blood coagulation III. The chemical constituents of blood platelets and their role in blood clotting, J Biol Chem, 116, 237, 10.1016/S0021-9258(18)74679-6 Murphy, 2012, Adult and umbilical cord blood-derived platelet-rich plasma for mesenchymal stem cell proliferation, chemotaxis, and cryo-preservation, Biomaterials, 33, 5308, 10.1016/j.biomaterials.2012.04.007 Dietz, 2007, Infrastructure development for human cell therapy translation, Clin Pharmacol Ther, 82, 320, 10.1038/sj.clpt.6100288 Lysaght, 2011, Regulating autologous adult stem cells: the FDA steps up, Cell Stem Cell, 9, 393, 10.1016/j.stem.2011.09.013 Hernigou, 2005, Percutaneous autologous bone-marrow grafting for nonunions. Influence of the number and concentration of progenitor cells, J Bone Joint Surg Am, 87, 1430 Hernigou, 2002, Treatment of osteonecrosis with autologous bone marrow grafting, Clin Orthop Relat Res Number, 405, 14, 10.1097/00003086-200212000-00003 Hernigou, 2008, Percutaneous implantation of autologous bone marrow osteoprogenitor cells as treatment of bone avascular necrosis related to sickle cell disease, Open Orthop J, 2, 62, 10.2174/1874325000802010062 Pettine, 2015, Percutaneous injection of autologous bone marrow concentrate cells significantly reduces lumbar discogenic pain through 12 months, Stem Cells, 33, 146, 10.1002/stem.1845 Pettine, 2016, Treatment of discogenic back pain with autologous bone marrow concentrate injection with minimum two year follow-up, Int Orthop, 40, 135, 10.1007/s00264-015-2886-4 Hernigou, 2014, Biologic augmentation of rotator cuff repair with mesenchymal stem cells during arthroscopy improves healing and prevents further tears: a case-controlled study, Int Orthop, 38, 1811, 10.1007/s00264-014-2391-1 Mishra, 2014, Efficacy of platelet-rich plasma for chronic tennis elbow: a double-blind, prospective, multicenter, randomized controlled trial of 230 patients, Am J Sports Med, 42, 463, 10.1177/0363546513494359 Dragoo, 2014, Platelet-rich plasma as a treatment for patellar tendinopathy: a double-blind, randomized controlled trial, Am J Sports Med, 42, 610, 10.1177/0363546513518416 Rodeo, 2012, The effect of platelet-rich fibrin matrix on rotator cuff tendon healing: a prospective, randomized clinical study, Am J Sports Med, 40, 1234, 10.1177/0363546512442924 Cole, 2015, Hyaluronic acid versus platelet-rich plasma: double-blind randomized controlled trial comparing clinical outcomes and intra-articular biology for treatment of knee arthritis, Orthop J Sport Med, 3 Filardo, 2012, Platelet-rich plasma intra-articular injections for cartilage degeneration and osteoarthritis: single- versus double-spinning approach, Knee Surg Sport Traumatol Arthrosc, 20, 2082, 10.1007/s00167-011-1837-x Cassano, 2016, Knee Surg Sports Traumatol Arthrosc, 1 Rehman, 2013, Alpha-2-macroglobulin: a physiological guardian, J Cell Physiol, 228, 1665, 10.1002/jcp.24266 Caplan, 2016, MSCs: the sentinel and safe-guards of injury, J Cell Physiol, 231, 1413, 10.1002/jcp.25255 Muschler, 1997, Aspiration to obtain osteoblast progenitor cells from human bone marrow: the influence of aspiration volume, J Bone Joint Surg Am, 79, 1699, 10.2106/00004623-199711000-00012 Hernigou, 2013, Benefits of small volume and small syringe for bone marrow aspirations of mesenchymal stem cells, Int Orthop, 37, 2279, 10.1007/s00264-013-2017-z Peters, 2016, Biopsy needle advancement during bone marrow aspiration increases mesenchymal stem cell concentration, Front Vet Sci, 3, 1