Bioresorbable nanofiber‐based systems for wound healing and drug delivery: Optimization of fabrication parameters

Journal of Biomedical Materials Research - Part B Applied Biomaterials - Tập 70B Số 2 - Trang 286-296 - 2004
Dhirendra S. Katti1,2, Kyle Robinson3, Frank Ko3, Cato T. Laurencin1,4,2
1Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia 22908
2Department of Orthopaedic Surgery, University of Virginia, Charlottesville, Virginia 22903
3Department of Materials Engineering, Drexel University, Philadelphia, Pennsylvania, 19104
4Department of Chemical Engineering, University of Virginia, Charlottesville, Virginia 22904

Tóm tắt

AbstractWound healing is a complex process that often requires treatment with antibiotics. This article reports the initial development of a biodegradable polymeric nanofiber‐based antibiotic delivery system. The functions of such a system would be (a) to serve as a biodegradable gauze, and (b) to serve as an antibiotic delivery system. The polymer used in this study was poly(lactide‐co‐glycolide) (PLAGA), and nanofibers of PLAGA were fabricated with the use of the electrospinning process. The objective of this study was to determine the effect of fabrication parameters: orifice diameter (needle gauge), polymer solution concentration, and voltage per unit length, on the morphology and diameter of electrospun nanofibers. The needle gauges studied were 16 (1.19 mm), 18 (0.84 mm), and 20 (0.58 mm), and the range of polymer solution concentration studied was from 0.10 g/mL to 0.30 g/mL. The effect of voltage was determined by varying the voltage per unit electrospinning distance, and the range studied was from 0.375 kV/cm to 1.5 kV/cm. In addition, the mass per unit area of the electrospun nanofibers as a function of time was determined and the feasibility of antibiotic (cefazolin) loading into the nanofibers was also studied. The results indicate that the diameter of nanofibers decreased with an increase in needle gauge (decrease in orifice diameter), and increased with an increase in the concentration of the polymer solution. The voltage study demonstrated that the average diameter of the nanofibers decreased with an increase in voltage. However, the effect of voltage on fiber diameter was less pronounced as compared to polymer solution concentration. The results of the areal density study indicated that the mass per unit area of the electrospun nanofibers increased linearly with time. Feasibility of drug incorporation into the nanofibers was demonstrated with the use of cefazolin, a broad‐spectrum antibiotic. Overall, these studies demonstrated that PLAGA nanofibers can be tailored to desired diameters through modifications in processing parameters, and that antibiotics such as cefazolin can be incorporated into these nanofibers. Therefore, PLAGA nanofibers show potential as antibiotic delivery systems for the treatment of wounds. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 70B: 286–296, 2004

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Journal of Biomedical Materials Research - Part B Applied Biomaterials

Tập 70B Số 2

286-296

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