Biorefined grass‐clover protein composition and effect on organic broiler performance and meat fatty acid profile

Journal of Animal Physiology and Animal Nutrition - Tập 104 Số 6 - Trang 1757-1767 - 2020
Lene Stødkilde1, Morten Ambye‐Jensen2, Søren Krogh Jensen1
1Department of Animal Science, Aarhus University, Foulum, Tjele, Denmark
2Department of Engineering, Aarhus University, Aarhus, Denmark

Tóm tắt

AbstractProtein extracted from green biomass can be a sustainable and valuable feed component for organic poultry production. Earlier studies in rats have shown high digestibility of laboratory‐scale extracted protein. The aim of this study was to test the effect of upscaling the biorefining process on composition of protein extracted from organic grass‐clover and on performance of organic broilers when including grass‐clover in the feed. Crude protein content of the extracted grass‐clover protein was 36.2% of dry matter (DM) with a higher methionine content, but lower lysine and total sulphur‐containing amino acids than that in soybean. Acid‐insoluble residue constituted a major fraction of the dietary fibre content, and a large proportion of total CP was bound in this fraction. Alpha‐linolenic acid was the dominating fatty acid in the extracted grass‐clover protein. One‐day‐old organic Colour Yield broiler chicks were included in a dose–response trial with grass‐clover protein constituting 0%, 8%, 16% or 24% of the feed from day 12 and until slaughter at day 57. Increasing levels of grass‐clover protein extract reduced feed intake, growth and slaughter weight; however, at 8% inclusion feed intake and performance were not affected. The fatty acid composition in broiler breast meat reflected the composition of grass‐clover protein extract; thus, the increasing dietary addition increased meat alpha‐linolenic acid content. A lowered tocopherol content in meat from broilers fed increasing grass‐clover protein demonstrated the need for increased amounts of antioxidants due to the high content of unsaturated fat. In conclusion, the study shows that broilers can grow on grass‐clover protein from an upscaled biorefining process, but highlights the importance of further optimisation with focus on increased protein content and on avoiding formation of insoluble protein complexes, as these most likely reduce protein digestibility.

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Tài liệu tham khảo

10.1002/fsn3.121

10.3382/ps.0620505

AOAC, 1990, Official methods of analysis of AOAC international

10.1016/S0377-8401(97)00009-6

10.1016/S0733-5210(87)80053-X

10.5713/ajas.2012.12178

10.1139/o59-099

10.4141/A02-098

10.3382/japr/pfw012

10.1002/bit.260230624

10.1016/j.anifeedsci.2018.08.004

10.1080/00071667008415827

10.1038/24376

10.1039/an9820700307

European Commission, 1998, Commission Directive 98/64/EC of 3 September 1998: Establishing Community methods of analysis for the determination of amino‐acids, crude oils and fats, and olaquindox in feedingstuffs, and amending Directive 71/393/EEC, 14

European Commission, 2000, Commission Directive 2000/45/EC of 6 July 2000 establishing Community methods of analysis for the determination of vitamin A, vitamin E and tryptophan in feedingstuffs, Official Journal of the European Communities

European Commission, 2014, COMMISSION IMPLEMENTING REGULATION (EU) No 836/2014 of 31 July 2014

European Commission, 2016, Facts and figures on organic agriculture in the European Union

Fanatico A. C., 2010, Organic poultry production: Providing adequate methionine

10.3382/japr.2008-00123

10.1093/ps/84.8.1321

10.22358/jafs/65686/2014

10.1007/978-1-4757-0022-0_29

10.1080/00015128909438504

Hubbard L. L. C., 2004, Management guide for Broilers

10.1002/jsfa.2740270510

10.1002/jsfa.2740320219

10.1002/jsfa.2740280307

10.1016/j.aninu.2017.08.003

10.1016/j.foodchem.2014.12.013

10.1017/S0022029900028247

10.1002/lite.200800074

10.1080/09064709809362400

10.1017/S0022029900032106

10.2527/jas.2012-5328

10.1093/japr/9.2.223

10.3382/ps.0561510

10.22358/jafs/70194/2017

Larsson K., 1983, Metodebeskrivning nr. 22

Leesons S., 1997, Commercial Poultry Nutrition

10.1021/jf60138a013

10.1111/j.1750-3841.2010.02038.x

10.1016/j.agrformet.2016.11.245

10.1021/jf8004367

10.1111/j.1745-4514.1984.tb00322.x

National Research Council, 2012, Nutrient requirements of swine

10.1016/j.foodres.2013.02.009

10.1021/jf405552m

10.1126/science.152.3730.1701

Pirie N. W., 1978, Leaf Protein and its by‐products in human and animal nutrition

10.3989/gya.1299142

10.1002/bbb.1957

10.1016/j.jclepro.2017.06.115

SEGES Svineproduktion, 2018, SEGES Fodermiddeltabel (Danish)

10.1093/ps/79.7.961

10.3390/nu8030128

10.3382/ps/pey323

10.1002/jsfa.8744

Stødkilde L., 2019, Digestibility of fractionated green biomass as protein source for monogastric animals, Animal, 1

10.3382/japr/pfv048

10.1111/j.1745-4514.2003.tb00289.x