Tối Ưu Hóa Quy Trình Sinh Học Của Penicillium funiculosum NCIM 1228 Để Nâng Cao Sản Xuất Và Hiệu Suất Thủy Phân Của Cellulase Trên Biomass Lignocellulosic Từ Mía

Sugar Tech - 2023
Sambhaji B. Chavan1,2,3, Ashvini M. Shete1, Mahesh S. Dharne2,3
1Praj Matrix-R & D Center (Division of Praj Industries Limited), Pune, India
2National Collection of Industrial Microorganisms (NCIM), Biochemical Sciences Division, CSIR-National Chemical Laboratory (NCL), Pune, India
3Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India

Tóm tắt

Enzyme cellulase hiện là enzyme thương mại lớn thứ ba trên thế giới. Do nhu cầu của nó trong các ngành công nghiệp khác nhau như dệt may, thực phẩm, quản lý chất thải, dược phẩm, nông nghiệp, giấy bột, nhiên liệu sinh học và các lĩnh vực khác, đường cầu của nó đã tăng nhanh chóng. Phương pháp thiết kế bề mặt phản ứng (RSM) đã được sử dụng để tối ưu hóa các thành phần môi trường và các thông số quy trình trong nghiên cứu hiện tại, điều này đã thành công trong việc tăng cường sản xuất cellulase từ Penicillium funiculosum NCIM 1228. Tối ưu hóa thống kê cho sự hyperproduction của cellulase đã được tiến hành bằng cách sử dụng RSM. Phương pháp thiết kế Plackett-Burman (PBD) đã được sử dụng để khảo sát các yếu tố quan trọng của môi trường sản xuất cellulase. Tiếp theo, phương pháp thiết kế Box-Behnken (BBD) đã được sử dụng để ước lượng thống kê các giá trị tối ưu và điều kiện có ảnh hưởng đáng kể đến sản xuất cellulase. Các tổ hợp thông số tối ưu ước lượng cho sản xuất cellulase là ure (0,2%), CaCl2 (0,2%), MgSO4 (0,05%), pepton (1,5%), cellulose vi tinh thể (5,0%), cám lúa mì (2,5%), dịch kéo ngô (CSL) (2,5%), KH2PO4 (0,15%), giống (10,65%), khuấy trộn (157 rpm), pH (5,88) và nhiệt độ (29,84 °C). Kết luận, xác thực thực nghiệm dưới các điều kiện tối ưu đã phát hiện một sự gia tăng trong sản xuất 3,82- và 3,61 lần trong thử nghiệm giấy lọc (FPase) và β-glucosidase, tương ứng. Ngoài ra, sự gia tăng 1,66- và 1,57 lần trong hoạt động cụ thể của FPase và β-glucosidase cũng được quan sát thấy, trong khi hoạt độ xylanase đã tăng 3,29 lần. Hơn nữa, enzyme cho thấy hiệu suất thủy phân đạt 51,30 phần trăm trên biomass lignocellulosic từ bã mía (LCB), với liều 7 đơn vị FPase trên g cellulose. P. funiculosum NCIM 1228 mang lại lợi ích trong việc sản xuất cellulase với một hệ thống enzym cellulolytic toàn diện có thể được tổng hợp ngoại bào, do đó hoạt động như một chất xúc tác sinh học hứa hẹn cho ngành công nghiệp nhiên liệu sinh học.

Từ khóa

#cellulase #Penicillium funiculosum #tối ưu hóa quy trình sinh học #phân hủy lignocellulosic #nhiên liệu sinh học

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