Bioprinting of novel 3D tumor array chip for drug screening
Tóm tắt
Biomedical field has been seeking a feasible standard drug screening system consisting of 3D tumor model array for drug researching due to providing sufficient samples and simulating actual in vivo tumor growth situation, which is still a challenge to rapidly and uniformly establish though. Here, we propose a novel drug screening system, namely 3D tumor array chip with “layer cake” structure, for drug screening. Accurate gelatin methacryloyl hydrogel droplets (~ 0.1 μL) containing tumor cells can be automatically deposited on demand with electrohydrodynamic 3D printing. Transparent conductive membrane is introduced as a chip basement for preventing charges accumulation during fabricating and convenient observing during screening. Culturing chambers formed by stainless steel and silicon interlayer is convenient to be assembled and recycled. As this chip is compatible with the existing 96-well culturing plate, the drug screening protocols could keep the same as convention. Important properties of this chip, namely printing stability, customizability, accuracy, microenvironment, tumor functionalization, are detailly examined. As a demonstration, it is applied for screening of epirubicin and paclitaxel with breast tumor cells to confirm the compatibility of the proposed screening system with the traditional screening methods. We believe this chip will potentially play a significant role in drug evaluation in the future.
Tài liệu tham khảo
DeVita VT, Chu E (2008) A history of cancer chemotherapy. Cancer Res 68:8643. https://doi.org/10.1158/0008-5472.CAN-07-6611
Eglen RM, Randle DH (2015) Drug discovery goes three-dimensional: goodbye to flat high-throughput screening? Assay Drug Dev Technol 13:262–265. https://doi.org/10.1089/adt.2015.647
Santo VE, Rebelo SP, Estrada MF, Alves PM, Boghaert E, Brito C (2017) Drug screening in 3D in vitro tumor models: overcoming current pitfalls of efficacy read-outs. Biotechnol J 12:1600505. https://doi.org/10.1002/biot.201600505
Liu Y, Shao C, Bian F, Yu Y, Wang H, Zhao Y (2018) Egg component-composited inverse opal particles for synergistic drug delivery. ACS Appl Mater Interfaces 10:17058–17064. https://doi.org/10.1021/acsami.8b03483
Wu Z, Yu Y, Zou M, Liu Y, Bian F, Zhao Y (2018) Peanut-inspired anisotropic microparticles from microfluidics. Compos Commun 10:129–135. https://doi.org/10.1016/j.coco.2018.09.007
Yu Y, Fu F, Shang L, Cheng Y, Gu Z, Zhao Y (2017) Bioinspired helical microfibers from microfluidics. Adv Mater 29:1605765. https://doi.org/10.1002/adma.201605765
Zhang H, Liu Y, Wang J, Shao C, Zhao Y (2019) Tofu-inspired microcarriers from droplet microfluidics for drug delivery. Sci China Chem 62:87–94. https://doi.org/10.1007/s11426-018-9340-y
Zhao Y, Gu H, Xie Z, Shum HC, Wang B, Gu Z (2013) Bioinspired multifunctional janus particles for droplet manipulation. J Am Chem Soc 135:54–57. https://doi.org/10.1021/ja310389w
Billiet T, Vandenhaute M, Schelfhout J, Van Vlierberghe S, Dubruel P (2012) A review of trends and limitations in hydrogel-rapid prototyping for tissue engineering. Biomaterials 33:6020–6041. https://doi.org/10.1016/j.biomaterials.2012.04.050
Nam K-H, Smith AST, Lone S, Kwon S, Kim D-H (2014) Biomimetic 3D tissue models for advanced high-throughput drug screening. J Lab Autom 20:201–215. https://doi.org/10.1177/2211068214557813
Shin CS, Kwak B, Han B, Park K (2013) Development of an in vitro 3D tumor model to study therapeutic efficiency of an anticancer drug. Mol Pharm 10:2167–2175. https://doi.org/10.1021/mp300595a
Cheng R, Yan Y, Liu H, Chen H, Pan G, Deng L, Cui W (2018) Mechanically enhanced lipo-hydrogel with controlled release of multi-type drugs for bone regeneration. Appl Mater Today 12:294–308. https://doi.org/10.1016/j.apmt.2018.06.008
Modaresifar K, Hadjizadeh A, Niknejad H (2017) Design and fabrication of GelMA/chitosan nanoparticles composite hydrogel for angiogenic growth factor delivery. Artif Cells Nanomed Biotechnol. https://doi.org/10.1080/21691401.2017.1392970
Morales AW, Zhang YS, Aleman J, Alerasool P, Dokmeci MR, Khademhosseini A, Ye JY (2016) Label-free detection of protein molecules secreted from an organ-on-a-chip model for drug toxicity assays. In: SPIE BiOS. SPIE, p 5
Nichol JW, Koshy ST, Bae H, Hwang CM, Yamanlar S, Khademhosseini A (2010) Cell-laden microengineered gelatin methacrylate hydrogels. Biomaterials 31:5536–5544. https://doi.org/10.1016/j.biomaterials.2010.03.064
Nie J, Gao Q, Wang Y, Zeng J, Zhao H, Sun Y, Shen J, Ramezani H, Fu Z, Liu Z, Xiang M, Fu J, Zhao P, Chen W, He Y (2018) Vessel-on-a-chip with hydrogel-based microfluidics. Small 14:1802368. https://doi.org/10.1002/smll.201802368
Shao L, Gao Q, Xie C, Fu J, Xiang M, He Y (2019) Bioprinting of cell-laden microfiber: can it become a standard product? Adv Healthc Mater. https://doi.org/10.1002/adhm.201900014
Shao L, Gao Q, Zhao H, Xie C, Fu J, Liu Z, Xiang M, He Y (2018) Fiber-based mini tissue with morphology-controllable GelMA microfibers. Small. https://doi.org/10.1002/smll.201802187
Rose BJ, Pacelli S, Haj JA, Dua SH, Hopkinson A, White JL, Rose RF (2014) Gelatin-based materials in ocular tissue engineering. Materials (Basel). https://doi.org/10.3390/ma7043106
Xianbin D (2018) 3D bio-printing review. IOP Conf Ser Mater Sci Eng 301:12023
Yoon HJ, Shin SR, Cha JM, Lee S-H, Kim J-H, Do JT, Song H, Bae H (2016) Cold water fish gelatin methacryloyl hydrogel for tissue engineering application. PLoS ONE 11:e0163902. https://doi.org/10.1371/journal.pone.0163902
Gao Q, Niu X, Shao L, Zhou L, Lin Z, Sun A, Fu J, Chen Z, Hu J, Liu Y, He Y (2019) 3D printing of complex GelMA-based scaffolds with nanoclay. Biofabrication 11:35006. https://doi.org/10.1088/1758-5090/ab0cf6
Hassanzadeh P, Kazemzadeh-Narbat M, Rosenzweig R, Zhang X, Khademhosseini A, Annabi N, Rolandi M (2016) Ultrastrong and flexible hybrid hydrogels based on solution self-assembly of chitin nanofibers in gelatin methacryloyl (GelMA). J Mater Chem B 4:2539–2543. https://doi.org/10.1039/C6TB00021E
McBeth C, Lauer J, Ottersbach M, Campbell J, Sharon A, Sauer-Budge AF (2017) 3D bioprinting of GelMA scaffolds triggers mineral deposition by primary human osteoblasts. Biofabrication 9:15009. https://doi.org/10.1088/1758-5090/aa53bd
Yue K, Trujillo-de Santiago G, Alvarez MM, Tamayol A, Annabi N, Khademhosseini A (2015) Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels. Biomaterials 73:254–271. https://doi.org/10.1016/j.biomaterials.2015.08.045
Chen X, Bai S, Li B, Liu H, Wu G, Liu S, Zhao Y (2016) Fabrication of gelatin methacrylate/nanohydroxyapatite microgel arrays for periodontal tissue regeneration. Int J Nanomed 11:4707–4718. https://doi.org/10.2147/IJN.S111701
Peela N, Sam FS, Christenson W, Truong D, Watson AW, Mouneimne G, Ros R, Nikkhah M (2016) A three dimensional micropatterned tumor model for breast cancer cell migration studies. Biomaterials 81:72–83. https://doi.org/10.1016/j.biomaterials.2015.11.039
Yan X, Zhou L, Wu Z, Wang X, Chen X, Yang F, Guo Y, Wu M, Chen Y, Li W, Wang J, Du Y (2019) High throughput scaffold-based 3D micro-tumor array for efficient drug screening and chemosensitivity testing. Biomaterials 198:167–179. https://doi.org/10.1016/j.biomaterials.2018.05.020
Cui X, Dean D, Ruggeri ZM, Boland T (2010) Cell damage evaluation of thermal inkjet printed Chinese hamster ovary cells. Biotechnol Bioeng 106:963–969. https://doi.org/10.1002/bit.22762
Barrero A, Loscertales IG (2006) Micro- and nanoparticles via capillary flows. Annu Rev Fluid Mech 39:89–106. https://doi.org/10.1146/annurev.fluid.39.050905.110245
Fernández de la Mora J (2006) The fluid dynamics of Taylor Cones. Annu Rev Fluid Mech 39:217–243. https://doi.org/10.1146/annurev.fluid.39.050905.110159
Xie M, Gao Q, Zhao H, Nie J, Fu Z, Wang H, Chen L, Shao L, Fu J, Chen Z, He Y (2019) Electro-assisted bioprinting of low-concentration GelMA microdroplets. Small 15:1–10. https://doi.org/10.1002/smll.201804216
Plodinec M, Loparic M, Monnier CA, Obermann EC, Zanetti-Dallenbach R, Oertle P, Hyotyla JT, Aebi U, Bentires-Alj M, Lim RYH, Schoenenberger C-A (2012) The nanomechanical signature of breast cancer. Nat Nanotechnol 7:757–765. https://doi.org/10.1038/nnano.2012.167
Zhao X, Liu S, Yildirimer L, Zhao H, Ding R, Wang H, Cui W, Weitz D (2016) Injectable stem cell-laden photocrosslinkable microspheres fabricated using microfluidics for rapid generation of osteogenic tissue constructs. Adv Funct Mater 26:2809–2819. https://doi.org/10.1002/adfm.201504943
Deryugina EI, Quigley JP (2006) Matrix metalloproteinases and tumor metastasis. Cancer Metastasis Rev 25:9–34. https://doi.org/10.1007/s10555-006-7886-9
Jung HY, Fattet L, Yang J (2015) Molecular pathways: linking tumor microenvironment to epithelial-mesenchymal transition in metastasis. Clin Cancer Res 21:962. https://doi.org/10.1158/1078-0432.CCR-13-3173
Liang C, Xu L, Song G, Liu Z (2016) Emerging nanomedicine approaches fighting tumor metastasis: animal models, metastasis-targeted drug delivery, phototherapy, and immunotherapy. Chem Soc Rev 45:6250–6269. https://doi.org/10.1039/C6CS00458J
Polacheck WJ, Zervantonakis IK, Kamm RD (2013) Tumor cell migration in complex microenvironments. Cell Mol Life Sci 70:1335–1356. https://doi.org/10.1007/s00018-012-1115-1
Wagenblast E, Soto M, Gutiérrez-Ángel S, Hartl CA, Gable AL, Maceli AR, Erard N, Williams AM, Kim SY, Dickopf S, Harrell JC, Smith AD, Perou CM, Wilkinson JE, Hannon GJ, Knott SRV (2015) A model of breast cancer heterogeneity reveals vascular mimicry as a driver of metastasis. Nature 520:358. https://doi.org/10.1038/nature14403https://www.nature.com/articles/nature14403%23supplementary-information
Aramaki T, Moriguchi M, Bekku E, Asakura K, Sawada A, Endo M (2013) Comparison of epirubicin hydrochloride and miriplatin hydrate as anticancer agents for transcatheter arterial chemoembolization of hepatocellular carcinoma. Hepatol Res 43:475–480. https://doi.org/10.1111/j.1872-034X.2012.01100.x
Lovitt CJ, Shelper TB, Avery VM (2015) Evaluation of chemotherapeutics in a three-dimensional breast cancer model. J Cancer Res Clin Oncol 141:951–959. https://doi.org/10.1007/s00432-015-1950-1
Lu CT, Zhao YZ, Wu Y, Tian XQ, Li WF, Huang PT, Li XK, Sun CZ, Zhang L (2011) Experiment on enhancing antitumor effect of intravenous epirubicin hydrochloride by acoustic cavitation in situ combined with phospholipid-based microbubbles. Cancer Chemother Pharmacol 68:343–348. https://doi.org/10.1007/s00280-010-1489-4
Zhang L, Li G, Gao M, Liu X, Ji B, Hua R, Zhou Y, Yang Y (2016) RGD-peptide conjugated inulin-ibuprofen nanoparticles for targeted delivery of Epirubicin. Colloids Surfaces B Biointerfaces 144:81–89. https://doi.org/10.1016/j.colsurfb.2016.03.077