Biomimetic Calcium Carbonate Concentric Microgrooves with Tunable Widths for Promoting MC3T3‐E1 Cell Functions

Advanced healthcare materials - Tập 2 Số 2 - Trang 326-333 - 2013
Xiaohui Wu1, Shanfeng Wang2,3
1Department of Materials Science and Engineering, The University of Tennessee, Knoxville,#N#TN 37996,#N#USA
2Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA
3Department of Materials Science and Engineering, The University of Tennessee, Knoxville, TN 37996, USA

Tóm tắt

Abstract

Biomimetic, self‐assembled calcium carbonate (CaCO3) concentric microgrooves with groove widths of 5.0 and 10 μm were fabricated through simply controlling incubation temperature. Mouse pre‐osteoblastic MC3T3‐E1 cells were cultured on flat and microgrooved substrates of CaCO3 and their adhesion, spreading, proliferation, alkaline phosphatase activity, and calcium content were remarkably enhanced by the microgrooves, in particular, the narrower ones. Furthermore, focal adhesions and actin filaments of MC3T3‐E1 cells could be aligned on both 5.0‐μm and 10‐μm‐wide CaCO3 grooves. Compared with the original round nuclei on the flat substrates and expanded round nuclei on the narrower microgrooves, the MC3T3‐E1 cell nuclei on 10‐μm‐wide CaCO3 grooves demonstrated preferred entrapment in the grooves and significant alignment with a smaller area after two‐day culture.

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Thông tin
Thông tin xuất bản

Advanced healthcare materials

Tập 2 Số 2

326-333

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