Biomethanol Production from Methane by Immobilized Co-cultures of Methanotrophs

Springer Science and Business Media LLC - Tập 60 - Trang 318-324 - 2020
Sanjay K. S. Patel1, Rahul K. Gupta1, Virendra Kumar1, Sanath Kondaveeti1, Anurag Kumar1, Devashish Das1, Vipin Chandra Kalia1, Jung-Kul Lee1
1Department of Chemical Engineering, Konkuk University, Seoul, Republic of Korea

Tóm tắt

Methanol production by co-culture of methanotrophs Methylocystis bryophila and Methyloferula stellata was examined from methane, a greenhouse gas. Co-culture exhibited higher methanol yield of 4.72 mM at optimum ratio of M. bryophila and M. stellata (3:2) compared to individual cultures. The immobilized co-culture within polyvinyl alcohol (PVA) showed relative efficiency of 90.1% for methanol production at polymer concentration of 10% (v/v). The immobilized co-culture cells within PVA resulted in higher bioprocess stability over free cells at different pH, and temperatures. Free and encapsulated co-cultures showed maximum methanol production of 4.81 and 5.37 mM under optimum conditions, respectively. After five cycles of reusage under batch conditions, free and encapsulated co-cultures retained methanol production efficiency of 23.8 and 61.9%, respectively. The present investigation successfully revealed the useful influence of co-culture on the methanol production over pure culture. Further, encapsulation within the polymeric matrix proved to be a better approach for the enhanced stability of the bioprocess.

Tài liệu tham khảo

Fei Q, Guarnieri MT, Tao L, Laurens LML, Dowe N, Pienkos PT (2014) Bioconversion of natural gas to liquid fuel: opportunities and challenges. Biotechnol Adv 32:596–614. https://doi.org/10.1016/j.biotechadv.2014.03.011 Patel SKS, Mardina P, Kim S-Y, Lee J-K, Kim I-W (2016) Biological methanol production by a type II methanotroph Methylocystis bryophila. J Microbiol Biotechnol 26:717–724. https://doi.org/10.4014/jmb.1601.01013 Patel SKS, Selvaraj C, Mardina P, Jeong JH, Kalia VC, Kang YC, Lee J-K (2016) Enhancement of methanol production from synthetic gas mixture by Methylosinus sporium through covalent immobilization. Appl Energy 171:383–391. https://doi.org/10.1016/j.apenergy.2016.03.022 Kondaveeti S, Patel SKS, Pagolu R, Li J, Kalia VC, Choi M-S, Lee J-K (2019) Conversion of simulated biogas to electricity: sequential operation of methanotrophic reactor effluents in microbial fuel cell. Energy 189:116309. https://doi.org/10.1016/j.energy.2019.116309 Pieja AJ, Morse MC, Cal AJ (2017) Methane to bioproducts: the future of the bioeconomy? Curr Opin Chem Biol 41:123–131. https://doi.org/10.1016/j.cbpa.2017.10.024 Patel SKS, Kondaveeti S, Otari SV, Pagolu RT, Jeong SH, Kim SC, Cho BK, Kang YC, Lee J-K (2018) Repeated batch methanol production from a simulated biogas mixture using immobilized Methylocystis bryophila. Energy 145:477–485. https://doi.org/10.1016/j.energy.2017.12.142 Patel SKS, Jeon MS, Gupta RK, Jeon Y, Kalia VC, Kim SC, Cho B-K, Kim DR, Lee J-K (2019) Hierarchical macro-porous particles for efficient whole-cell immobilization: application in bioconversion of greenhouse gases to methanol. ACS Appl Mater Interfaces 11:18968–18977. https://doi.org/10.1021/acsami.9b03420 Strong PJ, Kalyuzhnaya M, Silverman J, Clarke WP (2016) A methanotrophs-based biorefinery: potential scenarios for generating multiple products from a single fermentation. Bioresour Technol 215:314–323. https://doi.org/10.1016/j.biortech.2016.04.099 Patel SKS, Mardina P, Kim D, Kim S-Y, Kalia VC, Kim I-W, Lee J-K (2016) Improvement in methanol production by regulating the composition of synthetic gas mixture and raw biogas. Bioresour Technol 218:202–208. https://doi.org/10.1016/j.biortech.2016.06.065 Cantera S, Sánchez-Andrea I, Lebrero R, García-Encina PA, Stams AJM, Muñoz R (2018) Multi-production of high added market value metabolites from diluted methane emissions via methanotrophic extremophiles. Bioresour Technol 267:401–407. https://doi.org/10.1016/j.biortech.2018.07.057 Senko O, Makhlis T, Bihovsky M, Podmasterev V, Efremenko E, Razumovsky S, Varfolomeyev S (2007) Methanol production in the flow system with immobilized cells Methylosinus sporium, XV International Workshop on Bioencapsulation. Vienna, Austria. September 6–8, pp. 2–16, 1–4. https://impascience.eu/bioencapsulation/340_contribution_texts/2007-09-06_P2-16.pdf Yoo Y-S, Hana J-S, Ahn C-M, Kim C-G (2015) Comparative enzyme inhibitive methanol production by Methylosinus sporium from simulated biogas. Environ Technol 36:983–991. https://doi.org/10.1080/09593330.2014.971059 Taylor A, Molzahn P, Bushnell T, Cheney C, LaJeunesse M, Azizian M, Semprni L (2018) Immobilization of Methylosinus trichosporium OB3b for methanol production. J Ind Microbiol Biotechnol 45:201–211. https://doi.org/10.1007/s10295-018-2010-z Mardina P, Li J, Patel SKS, Kim I-W, Lee J-K, Selvaraj C (2016) Potential of immobilized whole-cell Methylocella tundrae as a biocatalyst for methanol production from methane. J Microbiol Biotechnol 26:1234–1241. https://doi.org/10.4014/jmb.1602.02074 Patel SKS, Singh R, Kumar A, Jeong JH, Jeong SH, Kalia VC, Kim I-W, Lee J-K (2017) Biological methanol production by immobilized Methylocella tundrae using simulated biohythane as a feed. Bioresour Technol 241:922–927. https://doi.org/10.1016/j.biortech.2017.05.160 Patel SKS, Kumar V, Mardina P, Li J, Lestari R, Kalia VC, Lee J-K (2018) Methanol production from simulated biogas mixtures by co-immobilized Methylomonas methanica and Methylocella tundrae. Bioresour Technol 263:25–32. https://doi.org/10.1016/j.biortech.2018.04.096 Kumar V, Patel SKS, Gupta RK, Otari SV, Gao H, Lee JK, Zhang L (2019) Enhanced saccharification and fermentation of rice straw by reducing the concentration of phenolic compounds using an immobilized enzyme cocktail. Biotechnol J 14:1800468. https://doi.org/10.1002/biot.201800468 Kumar A, Park GD, Patel SKS, Kondaveeti S, Otari S, Anwar MZ, Kalia VC, Singh Y, Kim SC, Cho B-K, Sohn J-H, Kim DR, Kang YC, Lee J-K (2019) SiO2 microparticles with carbon nanotube-derived mesopores as an efficient support for enzyme immobilization. Chem Eng J 359:1252–1264. https://doi.org/10.1016/j.cej.2018.11.052 Kondaveeti S, Patel SKS, Woo J, Wee JH, Kim S-Y, Al-Raoush RI, Kim I-W, Kalia VC, Lee J-K (2020) Characterization of cellobiohydrolases from Schizophyllum commune KMJ820. Indian J Microbiol 60:160–166. https://doi.org/10.1007/s12088-019-00843-9 Pandey D, Patel SKS, Singh R, Kumar P, Thakur V, Chand D (2019) Solvent-tolerant acyltransferase from Bacillus sp. APB-6: purification and characterization. Indian J Microbiol 59:500–507. https://doi.org/10.1007/s12088-019-00836-8 Patel SKS, Gupta RK, Kumar V, Mardina P, Lestari R, Kalia VC, Choi M-S, Lee J-K (2019) Influence of metal ions on the immobilization of β-glucosidase through protein-inorganic hybrids. Indian J Microbiol 59:370–374. https://doi.org/10.1007/s12088-019-0796-z Patel SKS, Choi H, Lee J-K (2019) Multimetal-based inorganic–protein hybrid system for enzyme immobilization. ACS Sustain Chem Eng 7:13633–13638. https://doi.org/10.1021/acssuschemeng.9b02583 Lee J-K, Patel SKS, Sung BH, Kalia VC (2020) Biomolecules from municipal and food industry wastes: an overview. Bioresour Technol 298:122346. https://doi.org/10.1016/j.biortech.2029.122346 Otari SV, Patel SKS, Kalia VC, Lee J-K (2020) One-step hydrothermal synthesis of magnetic rice straw for effective lipase immobilization and its application in esterification reaction. Bioresour Technol 302:122887. https://doi.org/10.1016/j.biortech.2020.122887 Patel SKS, Purohit HJ, Kalia VC (2010) Dark fermentative hydrogen production by defined mixed microbial cultures immobilized on ligno-cellulosic waste materials. Int J Hydrog Energy 35:10674–10681. https://doi.org/10.1016/j.ijhydene.2010.03.025 Patel SKS, Kumar P, Mehariya S, Purohit HJ, Lee J-K, Kalia VC (2014) Enhancement in hydrogen production by co-cultures of Bacillus and Enterobacter. Int J Hydrog Energy 39:14663–14668. https://doi.org/10.1016/j.ijhydene.2014.07.084 Kumar P, Sharma R, Ray S, Mehariya S, Patel SKS, Lee J-K, Kalia VC (2015) Dark fermentative bioconversion of glycerol to hydrogen by Bacillus thuringiensis. Bioresour Technol 182:383–388. https://doi.org/10.1016/j.biortech.2015.01.138 Patel SKS, Kumar P, Singh S, Lee J-K, Kalia VC (2015) Integrative approach to produce hydrogen and polyhydroxybutyrate from biowaste using defined bacterial cultures. Bioresour Technol 176:136–141. https://doi.org/10.1016/j.biortech.2014.11.029 Patel SKS, Lee J-K, Kalia VC (2017) Dark-fermentative biological hydrogen production from mixed biowastes using defined mixed cultures. Indian J Microbiol 57:171–176. https://doi.org/10.1007/s12088-017-0643-7 Patel SKS, Ray S, Prakash J, Wee JH, Kim S-Y, Lee J-K, Kalia VC (2019) Co-digestion of biowastes to enhance biological hydrogen process by defined mixed bacterial cultures. Indian J Microbiol 59:154–160. https://doi.org/10.1007/s12088-018-00777-8 Patel SKS, Kim J-H, Kalia VC, Lee J-K (2019) Antimicrobial activity of amino-derivatized cationic polysaccharides. Indian J Microbiol 59:96–99. https://doi.org/10.1007/s12088-018-00764-7 Prakash J, Sharma R, Patel SKS, Kim IW, Kalia VC (2018) Biohydrogen production by co-digestion of domestic wastewater and biodiesel industry effluent. PLoS ONE 13:e0199059. https://doi.org/10.1371/journal.pone.0199059 Razumovsky SD, Efremenko EN, Makhlis TA, Senko OV, Bikhovsky MY, Podmasterev VV, Varfolomeev SD (2008) Effect of immobilization on the main dynamic characteristics of the enzymatic oxidation of methane to methanol by bacteria Methylosinus sporium B-2121. Russ Chem Bull Int Ed 57:1633–1636. https://doi.org/10.1007/s11172-008-0211-8 Han J-S, Ahn C-M, Mahanty B, Kim C-G (2013) Partial oxidative conversion of methane to methanol through selective inhibition of methanol dehydrogenase in methanotrophic consortium from landfill cover soil. Appl Biochem Biotechnol 171:1487–1499. https://doi.org/10.1007/s12010-013-0410-0 Patel SKS, Kalia VC, Joo JB, Kang YC, Lee J-K (2020) Biotransformation of methane into methanol by methanotrophs immobilized on coconut coir. Bioresour Technol 297:122433. https://doi.org/10.1016/j.biortech.2019.122433 Patel SKS, Shanmugam R, Kalia VC, Lee J-K (2020) Methanol production by polymer-encapsulated methanotrophs from simulated biogas in the presence of methane vector. Bioresour Technol 304:123022. https://doi.org/10.1016/j.biortech.2020.123022 Gao H, Li J, Sivakumar D, Kim T-S, Patel SKS, Kalia VC, Kim I-W, Zhang Y-W, Lee J-K (2019) NADH oxidase from Lactobacillus reuteri: a versatile enzyme for oxidized cofactor regeneration. Int J Biol Macromol 123:629–636. https://doi.org/10.1016/j.ijbiomac.2018.11.096 Singh RK, Singh R, Sivakumar D, Kondaveeti S, Kim T, Li J, Sung BH, Cho B-K, Kim DR, Kim SC, Kalia VC, Zhang Y-HPJ, Zhao H, Kang YC, Lee J-K (2018) Insights into cell-free conversion of CO2 to chemicals by a multienzyme cascade reaction. ACS Catal 8:11085–11093. https://doi.org/10.1021/acscatal.8b02646 Kondaveeti S, Kim I-W, Otari S, Patel SKS, Pagolu R, Losetty V, Kalia VC, Lee J-K (2019) Co-generation of hydrogen and electricity from biodiesel process effluents. Int J Hydrog Energy 44:27285–27296. https://doi.org/10.1016/j.ijhydene.2019.08.258 Kondaveeti S, Pagolu R, Patel SKS, Kumar A, Bisht A, Dad D, Kalia VC, Kim I-W, Lee J-K (2019) Bioelectrochemical detoxification of phenolic compounds during enzymatic pre-treatment of rice straw. J Microbiol Biotechnol 29:1760–1768. https://doi.org/10.4014/jmb.1909.09042 Su Z, Ge X, Zhang W, Wang L, Yu Z, Li Y (2017) Methanol production from biogas with a thermotolerant methanotrophic consortium isolated from an anaerobic digestion system. Energy Fuels 31:2970–2975. https://doi.org/10.1021/acs.energyfuels.6b03471 AlSayed A, Fergala A, Khattab S, Elsharkawy A, Eldyasti A (2018) Optimization of methane bio-hydroxylation using waste activated sludge mixed culture of type I methanotrophs as biocatalyst. Appl Energy 211:755–763. https://doi.org/10.1016/j.apenergy.2017.11.090 Kim I-T, Yoo Y-S, Yoon Y-H, Lee Y-E, Jo J-H, Jeong W, Kim K-S (2018) Bio-methanol production using treated domestic wastewater with mixed methanotroph species and anaerobic digester biogas. Water 10:1414. https://doi.org/10.3390/w10101414 Markowska A, Michalkiewicz B (2009) Biosynthesis of methanol from methane by Methylosinus trichosporium OB3b. Chem Pap 63:105–110. https://doi.org/10.2478/s11696-008-000-5 Xin J-Y, Cui J-R, Niu J-Z, Hua S-F, Xia C-G, Li S-B, Zhu L-M (2004) Biosynthesis of methanol from CO2 and CH4 by methanotrophic bacteria. Biotechnology 3:67–71. https://doi.org/10.3923/biotech.2004.67.71 Patel SKS, Jeong J-H, Mehariya S, Otari SV, Madan B, Haw JR, Lee J-K, Zhang L, Kim I-W (2016) Production of methanol from methane by encapsulated Methylosinus sporium. J Microbiol Biotechnol 26:2098–2105. https://doi.org/10.4014/jmb.1608.08053