Biodegradation of DDT using multi‐species mixtures: From genome‐mining prediction to practical assessment

Microbial Biotechnology - Tập 17 Số 9 - 2024
Phuong Ha Vu1, Nguyen Huy Duong1, Tung Son Vu1, Anh H. Le1, Trang Trần1, Nguyen Thi Hong Yen1, Thuy Thu Thi Nguyen1, Linh Dam Thi2, Ha Viet Thi Bui2, Hanh My Tran2, Quang Huy Nguyen3, Thao Kim Nu Nguyen4, Bao Truong5, Huyen Thanh Thi Tran2, Hai The Pham2,1
1Research group for Physiology and Applications of Microorganisms (PHAM group), GREENLAB, Center for Life Science Research (CELIFE), Faculty of Biology, VNU University of Science – Vietnam National University, Hanoi, Vietnam
2Department of Microbiology, Faculty of Biology VNU University of Science – Vietnam National University Hanoi Vietnam
3Department of Biochemistry and Molecular Biology, Faculty of Biology, and Key Laboratory of Enzyme and Protein Technology VNU University of Science – Vietnam National University Hanoi Vietnam
4Department of Cell Biology, Faculty of Biology VNU University of Science – Vietnam National University Hanoi Vietnam
5High School for Gifted Students VNU University of Science – Vietnam National University Hanoi Vietnam

Tóm tắt

AbstractDDT (dichlorodiphenyltrichloroethane) is a commonly used insecticide that is recalcitrant and highly stable in the environment. Currently, DDT residue contamination, especially in agricultural soil, is still a concern in many countries, threatening human health and the environment. Among the approaches to resolve such an issue, novel biodegradation‐based methods are now preferred to physicochemical methods, due to the sustainability and the effectiveness of the former. In this study, we explored the possibility of building mixed microbial cultures that can offer improved DDT‐degrading efficiencies and be more environmentally transilient, based on genome annotation using the KEGG database and prediction of interactions between single strains using the obtained metabolic maps. We then proposed 10 potential DDT‐degrading mixed cultures of different strain combinations and evaluated their DDT degradation performances in liquid, semi‐solid and solid media. The results demonstrated the superiority of the mixtures over the single strains in terms of degrading DDT, particularly in a semi‐solid medium, with up to 40–50% more efficiency. Not only did the mixed cultures degrade DDT more efficiently, but they also adapted to broader spectra of environmental conditions. The three best DDT‐degrading and transilient mixtures were selected, and it turned out that their component strains seemed to have more metabolic interactions than those in the other mixtures. Thus, our study demonstrates the effectiveness of exploiting genome‐mining techniques and the use of constructed mixed cultures in improving biodegradation.

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Tài liệu tham khảo

10.1186/1471-2105-14-112

10.1016/j.jece.2018.03.030

10.1021/ac60122a018

Bhatt P., 2019, Smart bioremediation technologies: microbial enzymes

Brock T.D., 2003, Brock biology of microorganisms

10.1016/j.advwatres.2012.04.001

10.1016/j.jenvman.2011.06.047

10.1016/j.chemosphere.2024.141806

10.3389/fsufs.2021.754988

10.1002/iub.1324

10.1007/s40726-015-0023-z

10.1016/j.ecoenv.2023.114697

10.1016/j.ecoenv.2013.03.020

Deroiné M., 2015, Natural degradation and biodegradation of poly (3‐hydroxybutyrate‐co‐3‐hydroxyvalerate), Liquid and Solid Marine Environments, 23, 493

10.1007/s11270-008-9914-6

10.1186/s13068-016-0651-6

10.1016/j.envpol.2021.116824

10.1016/j.envpol.2023.123043

10.1007/s10158-006-0036-9

10.1007/s12223-020-00848-8

10.1016/S0065-2911(08)60146-0

10.1007/s00284-003-4053-1

10.1016/j.procbio.2022.04.015

10.1002/bit.24857

10.1016/j.chemosphere.2022.134776

10.1186/s12864-022-08623-4

10.1007/s00128-015-1596-3

10.1007/s12010-016-2214-5

10.1080/09593330.2019.1643926

10.1038/srep21332

10.1093/bioinformatics/btu153

Sharma A., 2019, Worldwide pesticide usage and its impacts on ecosystem, Applied Sciences, 1, 1

10.1111/j.1574-6976.2011.00269.x

10.1016/j.scitotenv.2018.10.441

10.1016/B978-0-12-417010-0.00005-7

10.1016/j.marpolbul.2019.05.009

10.3390/agriculture12050728

10.1016/j.scitotenv.2017.03.004

WHO, 1979, DDT and its derivatives

10.1016/j.proenv.2012.10.040

10.1016/j.chemosphere.2019.125392

10.1016/j.biortech.2020.123309

10.1371/journal.pone.0105307

10.1021/acs.est.8b06018

10.1039/C6NP00025H

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Microbial Biotechnology

Tập 17 Số 9

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