Biodegradation of 2,4-dinitrophenol with laccase immobilized on nano-porous silica beads
Tóm tắt
Many organic hazardous pollutants, including 2,4-dinitrophenol (2,4-DNP), which are water soluble, toxic, and not easily biodegradable make concerns for environmental pollution worldwide. In the present study, degradation of nitrophenols-contained effluents by using laccase immobilized on the nano-porous silica beads was evaluated. 2,4-DNP was selected as the main constituent of industrial effluents containing nitrophenols. The performance of the system was characterized as a function of pH, contact time, temperature, pollutant, and mediator concentrations. The laccase-silica beads were employed in a mixed-batch reactor to determine the degradation efficiency after 12 h of enzyme treatment. The obtained data showed that the immobilized laccase degraded more than 90% of 2,4-DNP within 12 h treatment. The immobilization process improved the activity and sustainability of laccase for degradation of the pollutant. Temperatures more than 50°C reduced the enzyme activity to about 60%. However, pH and the mediator concentration could not affect the enzyme activity. The degradation kinetic was in accordance with a Michaelis–Menten equation with Vmax and Km obtained as 0.25–0.38 μmoles/min and 0.13–0.017 mM, respectively. The stability of the immobilized enzyme was maintained for more than 85% of its initial activity after 30 days. Based on the results, it can be concluded that high resistibility and reusability of immobilized laccase on CPC-silica beads make it considerable choice for wastewater treatment.
Tài liệu tham khảo
Shukla SS, Dorris KL, Chikkaveeraiah BV: Photocatalytic degradation of 2, 4-dinitrophenol. J Hazard Mater. 2009, 164 (1): 310-314. 10.1016/j.jhazmat.2008.08.047.
Ahmadi Moghaddam M, Mesdaghinia A, Naddafi K, Nasseri S, Mahvi AH, Vaezi F: Degradation of 2, 4-dinitrophenol by photo fenton process. Asian J Chem. 2010, 22 (2): 1009-1016.
Harris MO, Cocoran J: Toxicological profile for dinitrophenols. 1995, Atlanta: Agency for Toxic Substances and Disease Registry
Dai R, Chen J, Lin J, Xiao S, Chen S, Deng Y: Reduction of nitro phenols using nitroreductase from E. coli in the presence of NADH. J Hazard Mater. 2009, 170 (1): 141-143. 10.1016/j.jhazmat.2009.04.122.
She ZL, Xie T, Li LL, Zhu YJ, Tang GF, Zhao YG: Study on the aerobic degradation kinetics of 2, 4-DNP and 2, 6-DNP. Adv Mater Res. 2012, 356: 186-189.
Champagne PP, Ramsay J: Dye decolorization and detoxification by laccase immobilized on porous glass beads. Bioresource Technol. 2010, 101 (7): 2230-2235. 10.1016/j.biortech.2009.11.066.
Hibi M, Hatahira S, Nakatani M, Yokozeki K, Shimizu S, Ogawa J: Extracellular oxidases of Cerrena sp. complementarily functioning in artificial dye decolorization including laccase, manganese peroxidase, and novel versatile peroxidases. Biocatal Agr Biotechnol. 2012, 1 (3): 220-225.
Kurniawati S, Nicell J: Variable stoichiometry during the laccase-catalyzed oxidation of aqueous phenol. Biotechnol Progr. 2007, 23 (2): 389-397. 10.1021/bp060312r.
Peralta-Zamora P, Pereira CM, Tiburtius ERL, Moraes SG, Rosa MA, Minussi RC: Decolorization of reactive dyes by immobilized laccase. Appl Catal B-Environ. 2003, 42 (2): 131-144. 10.1016/S0926-3373(02)00220-5.
Gholami-Borujeni F, Mahvi AH, Naseri S, Faramarzi MA, Nabizadeh R, Alimohammadi M: Application of immobilized horseradish peroxidase for removal and detoxification of azo dye from aqueous solution. Res J Chem Environ. 2011, 15 (2): 217-222.
Gholami-Borujeni F, Mahvi AH, Nasseri S, Faramarzi MA, Nabizadeh R, Alimohammadi M: Enzymatic treatment and detoxification of acid orange 7 from textile wastewater. Appl Biochem Biotech. 2011, 165 (5–6): 1274-1284.
Aghaie-Khouzani M, Forootanfar H, Moshfegh M, Khoshayand M, Faramarzi M: Decolorization of some synthetic dyes using optimized culture broth of laccase producing ascomycete Paraconiothyrium variabile. Biochem Eng J. 2011, 60: 9-15.
Forootanfar H, Movahednia MM, Yaghmaei S, Tabatabaei-Samani M, Rastegar H, Sadighi A: Removal of chlorophenolic derivatives by soil isolated ascomycete of Paraconiothyrium variabile and studying the role of its extracellular laccase. J Hazard Mater. 2012, 209–210: 199-203.
Rekuc A, Jastrzembska B, Liesiene J, Bryjak J: Comparative studies on immobilized laccase behaviour in packed-bed and batch reactors. J Mol Catal B: Enzym. 2009, 57 (1–4): 216-223.
Wang P, Fan X, Cui L, Wang Q, Zhou A: Decolorization of reactive dyes by laccase immobilized in alginate/gelatin blent with PEG. J Environ Sci. 2008, 20 (12): 1519-1522. 10.1016/S1001-0742(08)62559-0.
Jiang DS, Long SY, Huang J, Xiao HY, Zhou JY: Immobilization of Pycnoporus sanguineus laccase on magnetic chitosan microspheres. Biochem Eng J. 2005, 25 (1): 15-23. 10.1016/j.bej.2005.03.007.
Stanescu MD, Gavrilas S, Ludwig R, Haltrich D, Lozinsky VI: Preparation of immobilized Trametes pubescens laccase on a cryogel-type polymeric carrier and application of the biocatalyst to apple juice phenolic compounds oxidation. Eur Food Res Technol. 2012, 234 (4): 655-662. 10.1007/s00217-012-1676-0.
Nicolucci C, Rossi S, Menale C, Godjevargova T, Ivanov Y, Bianco M: Biodegradation of bisphenols with immobilized laccase or tyrosinase on polyacrylonitrile beads. Biodegradation. 2011, 22 (3): 673-683. 10.1007/s10532-010-9440-2.
Kalkan NA, Aksoy S, Aksoy EA, Hasirci N: Preparation of chitosan coated magnetite nanoparticles and application for immobilization of laccase. J Appl Polym Sci. 2012, 123 (2): 707-716. 10.1002/app.34504.
Gaitan IJ, Medina SC, González JC, Rodríguez A, Espejo ÁJ, Osma JF: Evaluation of toxicity and degradation of a chlorophenol mixture by the laccase produced by Trametes pubescens. Bioresource Technol. 2011, 102 (3): 3632-3635. 10.1016/j.biortech.2010.11.040.
Mogharabi M, Nassiri-Koopaei N, Bozorgi-Koushalshahi M, Nafissi-Varcheh N, Bagherzadeh G, Faramarzi MA: Immobilization of laccase in alginate-gelatin mixed gel and decolorization of synthetic dyes. Bioinor Chem Appl. 2012, 2012: 1-6.
Dai Y, Yin L, Niu J: Laccase-carrying electrospun fibrous membranes for adsorption and degradation of PAHs in shoal soils. Environ Sci Technol. 2011, 45 (24): 10611-10618. 10.1021/es203286e.
Catapane M, Nicolucci C, Menale C, Mita L, Rossi S, Mita DG, Diano N: Enzymatic removal of estrogenic activity of nonylphenol and octylphenol aqueous solutions by immobilized laccase from Trametes versicolor. J Hazard Mater. 2013, 248–249: 337-346.
Lante A, Crapisi A, Krastanov A, Spettoli P: Biodegradation of phenols by laccase immobilised in a membrane reactor. Process Biochem. 2000, 36: 51-58. 10.1016/S0032-9592(00)00180-1.
Bhattacharya S, Banerjee R: Laccase mediated biodegradation of 2, 4-dichlorophenol using response surface methodology. Chemosphere. 2008, 73 (1): 81-85. 10.1016/j.chemosphere.2008.05.005.
Aktaş N, Tanyolaç A: Reaction conditions for laccase catalyzed polymerization of catechol. Bioresource Technol. 2003, 87 (3): 209-214. 10.1016/S0960-8524(02)00254-7.
Okazaki S, Michizoe J, Goto M, Furusaki S, Wariishi H, Tanaka H: Oxidation of bisphenol A catalyzed by laccase hosted in reversed micelles in organic media. Enzyme Microb Tech. 2002, 31 (3): 227-232. 10.1016/S0141-0229(02)00104-7.
Rancano G, Lorenzo M, Molares N, Rodriguez Couto S, Sanromán MA: Production of laccase by Trametes versicolor in an airlift fermentor. Process Biochem. 2003, 39 (4): 467-473. 10.1016/S0032-9592(03)00083-9.
Liu Y: M.Sc thesis. Laccase-catalyzed oxidation of bisphenol a in a non-aqueous liquid using reverse micelles. 2004, Montreal: McGill University, Department of Civil Engineering and Applied Mechanics
Frasconi M, Favero G, Boer H, Koivula A, Mazzei F: Kinetic and biochemical properties of high and low redox potential laccases from fungal and plant origin. BBA-Proteins Proteomics. 2010, 1804 (4): 899-908. 10.1016/j.bbapap.2009.12.018.
Hong G, Ivnitski DM, Johnson GR, Atanassov P, Pachter R: Design parameters for tuning the type 1 CU multicopper oxidase redox potential: insight from a combination of first principles and empirical molecular dynamics simulations. J Am Chem Soc. 2011, 133 (13): 4802-4809. 10.1021/ja105586q.
Sadhasivam S, Savitha S, Swaminathan K, Lin FH: Production, purification and characterization of mid-redox potential laccase from a newly isolated Trichoderma harzianum WL1. Process Biochem. 2008, 43 (7): 736-742. 10.1016/j.procbio.2008.02.017.
Fabbrini M, Galli C, Gentili P: Comparing the catalytic efficiency of some mediators of laccase. J Mol Catal B: Enzym. 2002, 16 (5): 231-240.
D’Acunzo F, Galli C, Masci B: Oxidation of phenols by laccase and laccase-mediator systems. Eur J Biochem. 2002, 269 (21): 5330-5335. 10.1046/j.1432-1033.2002.03256.x.
Tavares APM, Rodríguez O, Fernández-Fernández M, Domínguez A, Moldes D, Sanromán MA, Macedo EA: Immobilization of laccase on modified silica: stabilization, thermal inactivation and kinetic behaviour in 1-ethyl-3-methylimidazolium ethylsulfate ionic liquid. Bioresource Technol. 2013, 131: 405-412.