Bioconversion of glycerol to 1,3-propanediol in thin stillage-based media by engineered Lactobacillus panis PM1
Tóm tắt
Thin stillage (TS) is a waste residue that remains after bioethanol production, and its disposal reflects the high costs of bioethanol production. Thus, the development of cost-effective ways to process TS is a pending issue in bioethanol plants. The aim of this study was to evaluate the utilization of TS for the production of the valuable chemical, 1,3-propanediol (1,3-PDO), by Lactobacillus panis PM1. Different fermentation parameters, including temperature, pH and strains [wild-type and a recombinant strain expressing a NADPH-dependent aldehyde reductase (YqhD) gene] were tested in batch and fed-batch cultivations. The highest 1,3-PDO concentration (12.85 g/L) and yield (0.84 g/g) were achieved by batch fermentation at pH-4.5/30 °C by the YqhD recombinant strain. Furthermore, pH-controlled batch fermentation reduced the total fermentation period, resulting in the maximal 1,3-PDO concentration of 16.23 g/L and yield of 0.72 g/g in TS without an expensive nutrient or nitrogen (e.g., yeast extract, beef extract, and peptone) supplementation. The addition of two trace elements, Mg2+ and Mn2+, in TS increased 1,3-PDO yield (0.74 g/g) without 3-hydroxypropionaldehyde production, the only intermediate of 1,3-PDO biosynthetic pathway in L. panis PM1. Our results suggest that L. panis PM1 can offer a cost-effective process that utilizes the TS to produce a value-added chemical, 1,3-PDO.
Tài liệu tham khảo
U.S. Energy Information Administration (2012) Annual energy review 2011, DOE/EIA-0384(2011). http://www.eia.gov/totalenergy/data/annual/archive/038411.pdf
Ahn J-H, Sang B-I, Um Y (2011) Butanol production from thin stillage using Clostridium pasteurianum. Bioresour Technol 102(7):4934–4937
Daniel R, Bobik TA, Gottschalk G (1998) Biochemistry of coenzyme B12-dependent glycerol and diol dehydratases and organization of the encoding genes. FEMS Microbiol Rev 22(5):553–566
Djukic-Vukovic AP, Mojovic LV, Jokic BM, Nikolic SB, Pejin JD (2013) Lactic acid production on liquid distillery stillage by Lactobacillus rhamnosus immobilized onto zeolite. Bioresour Technol 135:454–458
Jarboe LR (2011) YqhD: a broad-substrate range aldehyde reductase with various applications in production of biorenewable fuels and chemicals. Appl Microbiol Biotechnol 89(2):249–257
Kang TS, Korber DR, Tanaka T (2013) Contributions of citrate in redox potential maintenance and ATP production: metabolic pathways and their regulation in Lactobacillus panis PM1. Appl Microbiol Biotechnol 97(19):8693–8703
Kang TS, Korber DR, Tanaka T (2013) Glycerol and environmental factors: effects on 1, 3-propanediol production and NAD+ regeneration in Lactobacillus panis PM1. J Appl Microbiol 115(4):1003–1011
Kaur G, Srivastava AK, Chand S (2012) Advances in biotechnological production of 1,3-propanediol. Biochem Eng J 64:106–118
Khan NH, Kang TS, Grahame DA, Haakensen MC, Ratanapariyanuch K, Reaney MJ, Korber DR, Tanaka T (2013) Isolation and characterization of novel 1,3-propanediol-producing Lactobacillus panis PM1 from bioethanol thin stillage. Appl Microbiol Biotechnol 97(1):417–428
Kim Y, Mosier NS, Hendrickson R, Ezeji T, Blaschek H, Dien B, Cotta M, Dale B, Ladisch MR (2008) Composition of corn dry-grind ethanol by-products: DDGS, wet cake, and thin stillage. Bioresour Technol 99(12):5165–5176
Konings WN (2002) The cell membrane and the struggle for life of lactic acid bacteria. Antonie Van Leeuwenhoek 82(1–4):3–27
Li Z, Han L, Ji Y, Wang X, Tan T (2010) Fermentative production of l-lactic acid from hydrolysate of wheat bran by Lactobacillus rhamnosus. Biochem Eng J 49(1):138–142
Liang Y, Zhao X, Strait M, Wen Z (2012) Use of dry-milling derived thin stillage for producing eicosapentaenoic acid (EPA) by the fungus Pythium irregulare. Bioresour Technol 111:404–409
Nakamura CE, Whited GM (2003) Metabolic engineering for the microbial production of 1,3-propanediol. Curr Opin Biotechnol 14(5):454–459
Pflügl S, Marx H, Mattanovich D, Sauer M (2012) 1,3-Propanediol production from glycerol with Lactobacillus diolivorans. Bioresour Technol 119:133–140
Saxena RK, Anand P, Saran S, Isar J (2009) Microbial production of 1,3-propanediol: recent developments and emerging opportunities. Biotechnol Adv 27(6):895–913