Bio-prospecting of Waste Vegetable Resources for Isolation of Milk Clotting Proteases
Tóm tắt
Protease enzymes are extensively utilized in several industries ranging from pharmaceutical, food, biotechnology, cosmetics, leather, textile, dairy, and allied sectors. However, Plant rennet is in high demand in food and dairy industry due to several ethical, religious, and environmental causes. Jackfruit, pumpkin, and lotus seed extracts were tested for their milk clotting activities and found to have maximum milk clotting activity 2400MCU at their respective optimum conditions. Due to higher MCA milk curd were also tested for their potential use and found suitable for cheese preparation. Cottage cheese was prepared from all the plant extracts and for sensory analysis a panel of thirty volunteers were offered the sample and given the overall higher acceptability scores. In this view, more work is required, however an overall sensory acceptance suggested that these plant extracts can be used as an alternative to the animal rennet in dairy industry.
Tài liệu tham khảo
C.D.T. Freitas et al., Insights into milk-clotting activity of latex peptidases from Calotropis procera and Cryptostegia grandiflora. Food Res Int 87, 50–59 (2016)
P. Devilee, C.J. Cornelisse, Action on bovine asi-casein of cardosins A and B, aspartic proteinases from the flowers of the cardoon Cynara cardunculus L. Biochim Biophys Acta 1297, 83–89 (1996)
A. Kumar, S. Sasmal, Rheological and physico-chemical properties of milk gel using isolate of pumpkin (Cucurbita moschata) seeds: a new source of milk clotting peptidase. Food Hydrocoll 106, 105866 (2020)
P.F. Fox, T.P. Guinee, T.M. Cogan, P.L.H. McSweeney, Factors That Affect Cheese Quality”, in Fundamentals of Cheese Science (Springer, New York, 2017), pp. 553–542
S. Yildirim-Elikoglu, H. Vural, Y.K. Erdem, Effect of phenolic compounds on the activity of proteolytic enzymes during rennet induced coagulation of milk and ripening of miniature cheese. LWT - Food Sci. Technol 136, 110337 (2021)
K. Liburdi, C. Boselli, G. Giangolini, S. Amatiste, M. Esti, An Evaluation of the clotting properties of three plant rennets in the milks of different animal species. Foods 8(600), 1–12 (2019)
A. Salinas-Valdés, M.J. De la Rosa, S.O. Serna-Saldívar, C. Chuck-Hernández, Yield and textural characteristics of panela cheeses produced with dairy-vegetable protein (Soybean or Peanut) blends supplemented with Transglutaminase. J Food Sci 80(12), S2950–S2956 (2015)
T. Godifey, Comparative study of the coagulation effect of solanium nigrum and ficus palmata in yoghurt production. J Nat Sci Res 5(15), 61–65 (2015)
M. Benkahoul, M. Benchiheub, I. Bellil, D. Khelifi, A.M. Maza, Physical and chemical properties of the acid protease from Onopordum acanthium: comparison between electrophoresis and HPLC of degradation casein profiles. African J Biotechnol 15(9), 331–340 (2016)
E. Bueno-Gavilá et al., Bioactivity of hydrolysates obtained from bovine casein using artichoke (Cynara scolymus L.) proteases. J Dairy Sci 102(12), 10711–10723 (2019)
X. Wang, Y. Shi, R. He, B. Li, A. Huang, Label-free quantitative proteomic analysis of the biological functions of Moringa oleifera seed proteins provides insights regarding the milk-clotting proteases. Int J Biol Macromol 144, 325–333 (2020)
M. Oliveira, M. Ferreira, D. Morais, J. Ferreira, A2A2 milk: Brazilian consumers ’ opinions and e ff ect on sensory characteristics of Petit Suisse and Minas cheeses. LWT - Food Sci Technol 108, 207–213 (2019)
H. Swaisgood, Primary sequence of Kappa-Casein. J. Dairy Sci 58(4), 583–592 (1974)