Bio-Synthesis of Gold Nanoparticles by Fusarium oxysporum and Assessment of Their Conjugation Possibility with Two Types of β-Lactam Antibiotics without Any Additional Linkers
Tóm tắt
In the present study, biosynthesis of gold nanoparticles (GNPs) by Fusarium oxysporum was carried out and their conjugation possibility with two β-lactam antibiotics was evaluated. F. oxysporum was cultured and the fungal culture supernatant was subjected to the 1 mmol final concentration of chloroauric acid solution. The produced GNPs were analyzed using visible spectrophotometer, X-ray diffraction analysis (XRD) and transmission electron microscope (TEM). After the purification of GNPs, they were subjected to penicillin G and ceftriaxone without any additional linkers. Finally, the mixture was analyzed using visible spectrophotometer, Fourier transform infrared spectroscopy (FTIR) and TEM and subjected to antibacterial activity test using the well diffusion method. Results confirmed the presence of GNPs in the F. oxysporum culture supernatant after the addition of chloroauric acid. TEM results showed that GNPs were spherical and amorphous with sizes around 10–25 nm and XRD confirmed the presence of GNPs in the fungal culture supernatant. After the incubation of GNPs with the antibiotics, FTIR results demonstrated the successful linking of GNPs with the corresponded antibiotics and TEM images showed that GNPs sizes were bigger than the pure ones (around 50–100 nm). Finally the antibacterial activity test indicated that absolutely, the antibacterial properties of the GNPs-β-lactam antibiotics was lowered or not changed in contrast to the pure antibiotics. The present study showed that GNPs had high tendency of conjugation with antibiotics but unlike the previous researches, linking of the antibiotics to GNPs always cannot improve their antibacterial activity based on the antibiotics that were used. The high conjugation affinity of GNPs made them a good candidate as detoxification agent in diverse areas of medicine or environmental sciences.
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Alkilany, A.M. and Murphy, C.J., Toxicity and cellular uptake of gold nanoparticles: what we have learned so far?, J. Nanoparticle Res., 2010, vol. 12, pp. 2313–2333.
Burygin, G., Khlebtsov, B., Shantrokha, A., Dykman, L., Bogatyrev, V., and Khlebtsov, N., On the enhanced antibacterial activity of antibiotics mixed with gold nanoparticles, Nanoscale Res. Lett., 2009, vol. 4, pp. 794–801.
Fayaz, A.M., Girilal, M., Mahdy, S.A., Somsundar, S., Venkatesan, R., and Kalaichelvan, P., Vancomycin bound biogenic gold nanoparticles: a different perspective for development of anti VRSA agents, Proc. Biochem., 2011, vol. 46, pp. 636–641.
Finkelstein, A., Walz, D., Batista, V., Mizraji, M., Roisman, F., and Misher, A., Auranofin. New oral gold compound for treatment of rheumatoid arthritis, Ann. Rheum. Dis., 1976, vol. 35, pp. 251–257.
Gericke, M. and Pinches, A., Biological synthesis of metal nanoparticles, Hydrometallurgy, 2006, vol. 83, pp. 132–140.
Gordon, T. and Martyn, R., The evolutionary biology of Fusarium oxysporum, Ann. Rev. Phytopathol., 1997, vol. 35, pp. 111–128.
Gu, H., Ho, P., Tong, E., Wang, L., and Xu, B., Presenting vancomycin on nanoparticles to enhance antimicrobial activities, Nano Lett., 2003, vol. 3, pp. 1261–1263.
Jain, K.K., Nanomedicine: application of nanobiotechnology in medical practice, Med. Principl. Practice, 2008, vol. 17, pp. 89–101.
Khademi Mazdeh, S., Motamedi, H., Akbarzadeh Khiavi, A., and Reza Mehrabi, M., Gold nanoparticle biosynthesis by E. coli and conjugation with streptomycin and evaluation of its antibacterial effect, Curr. Nanosci., 2014, vol. 10, pp. 553–561.
Narayanan, K.B. and Sakthivel, N., Biological synthesis of metal nanoparticles by microbes, Adv. Colloid Interface Sci., 2010, vol. 156, pp. 1–13.
Nikbakht, M., Yahyaei, B., and Pourali, P., Green synthesis, characterization and antibacterial activity of silver nanoparticles using fruit aqueous and methanolic extracts of Berberis vulgaris and Ziziphus vulgaris, J. Pure Appl. Microbiol., 2015, vol. 9, pp. 309–355.
Pissuwan, D., Niidome, T., and Cortie, M.B., The forthcoming applications of gold nanoparticles in drug and gene delivery systems, J. Controlled Release, 2011, vol. 149, pp. 65–71.
Pourali, P., Baserisalehi, M., Afsharnezhad, S., Behravan, J., Alavi, H., and Hosseini, A., Biological synthesis of silver and gold nanoparticles by bacteria in different temperatures (37 and 50°C), J. Pure Appl. Microbiol., 2012, vol. 6, pp. 757–763.
Pourali, P., Baseri, S.M., Afsharnezhad, S., Behravan, J., Ganjali, R., Bahador, N., and Arabzadeh, S., Biological production and assessment of the antibacterial activity of gold nanoaprticles, Biometals, 2013, vol. 26, pp. 189–196.
Pourali, P., Yahyaei, B., Ajoudanifar, H., Taheri, R., Alavi, H., and Hoseini, A., Impregnation of the bacterial cellulose membrane with biologically produced silver nanoparticles, Curr. Microbiol., 2014, vol. 69, pp. 785–793.
Pourali, P. and Yahyaei, B., Biological production of silver nanoparticles by soil isolated bacteria and preliminary study of their cytotoxicity and cutaneous wound healing efficiency in rat, J. Trace Elements Med. Biol., 2016, vol. 34, pp. 22–31.
Pourali, P., Razavian Zadeh, N., and Yahyaei, B., Silver nanoparticles production by two soil isolated bacteria, Bacillus thuringiensis and Enterobacter cloacae, and assessment of their cytotoxicity and wound healing effect in rats, Wound Repair Regeneration, 2016, vol. 24, pp. 860–869.
Rastogi, L., Kora, A.J., and Arunachalam, J., Highly stable, protein capped gold nanoparticles as effective drug delivery vehicles for amino-glycosidic antibiotics, Mater. Sci. Eng.: C, 2012, vol. 32, pp. 1571–1577.
Roberts, J.A., Webb, S., Paterson, D., Ho, K.M., and Lipman, J., A systematic review on clinical benefits of continuous administration of β-lactam antibiotics, Critical Care Med., 2009, vol. 37, pp. 2071–2078.
Saha, B., Bhattacharya, J., Mukherjee, A., Ghosh, A., Santra, C., Dasgupta, A.K., and Karmakar, P., In vitro structural and functional evaluation of gold nanoparticles conjugated antibiotics, Nanoscale Res. Lett., 2007, vol. 2, pp. 614–622.
Siddiqi, K.S. and Husen, A., Fabrication of metal nanoparticles from fungi and metal salts: scope and application, Nanoscale Res. Lett., 2016, vol. 11, pp. 1–15.
Tansil, N.C. and Gao, Z., Nanoparticles in biomolecular detection, Nano Today, 2006, vol. 1, pp. 28–37.
Tom, R.T., Suryanarayanan, V., Reddy, P.G., Baskaran, S., and Pradeep, T., Ciprofloxacin-protected gold nanoparticles, Langmuir, 2004, vol. 20, pp. 1909–1914.
Vijayaraghavan, K., Mahadevan, A., Sathishkumar, M., Pavagadhi, S., and Balasubramanian, R., Biosynthesis of Au(0) from Au(III) via biosorption and bioreduction using brown marine alga Turbinaria conoides, Chem. Eng. J., 2011, vol. 167, pp. 223–227.
Yahyaei, B., Peyvandi, N., Akbari, H., Arabzadeh, S., Afsharnezhad, S., Ajoudanifar, H., and Pourali, P., Production, assessment, and impregnation of hyaluronic acid with silver nanoparticles that were produced by Streptococcus pyogenes for tissue engineering applications, Appl. Biol. Chem., 2016, vol. 59, pp. 227–237.