Baculovirus vectors for efficient gene delivery and expression in mammalian cells
Tóm tắt
Baculovirus expression vectors are extensively used for the delivery of foreign genes and expression of recombinant proteins in insect and mammalian cells. Modified baculoviruses containing mammalian promoter elements (BacMam viruses) for an efficient transient and stable transduction of diverse mammalian cells ensure a high level of heterologous protein expression both in vitro and in vivo. Recombinant baculovirus vectors containing mammalian expression cassette with cytomegalovirus promoter, green or red fluorescent protein gene, SV40pA polyadenylation signal, and polylinker MCS were constructed for the delivery of genes encoding hepatitis C virus structural proteins into mammalian cells. In HEK293T and Huh7 cells, formation of glycoprotein complexes and HCV4ike particles was observed. A high efficiency of the baculovirus-medi-ated gene transfer and expression of the virus envelope proteins in mammalian cells was demonstrated using fluorescence, flow cytometry, and immunoblot techniques.
Tài liệu tham khảo
Smith G.E., Summers M.D., Fraser M.J. 1983. Production of human beta interferon in insect cells infected with a baculovirus expression vector. Mol. Cell. Biol. 3, 2156–2165.
Carbonell L.F.R., Klowden M.J., Miller L.K. 1985. Baculovirus-mediated expression of bacterial genes in dipteran and mammalian cells. J. Virol. 56, 153–160.
Matsuura Y., Posse R.D., Overton H.A., Bishop D.H.L. 1987. Baculovirus expression vectors: The requirements for high level expression of proteins. J. Gen. Virol. 68, 1233–1250.
Luckow V.A., Summers M.D. 1988. Trends in the development of baculovirus expression vectors. Biotechnology. 6, 47–55.
Beljelarskaya S.N. 2002. A baculovirus expression system for insect cells. Mol. Biol. 36, 281–292.
Ernst W., Grabherr R., Wegner D., Borth N., Grassauer A., Katinger H. 1998. Baculovirus surface display: Construction and screening of a eukaryotic epitope library. Nucleic Acids Res. 26, 1718–1723.
Hofmann C., Sandig. V., Jennings G., Rudolph M., Schlag P., Strauss M. 1995. Efficient gene transfer into human hepatocytes by baculovirus vectors. Proc. Natl. Acad. Sci. USA. 92, 10099–10103.
Boyce FM., Bucher N.L.R. 1996. Baculovirus-medi-ated gene transfer into mammalian cells. Proc. Natl, Acad. Sci. USA. 93, 2348–2352.
Tjia S.T., zu Altenschildesche G.M., Doerfler W. 1983. Autographa californica nuclear polyhedrosis virus (AcNPV) DNA dose not persist in mass cultures of mammalian cells. Virology. 125, 107–117.
Shoji I., Aizaki H., Tani H., Ishii K., Chiba T., Saito I., Miyamura T., Matsuura Y. 1997. Efficient gene transfer into varios mammalian cells, including non-hepatic cells, by baculovirus vectors. J. Gen. Virol. 78, 2657–2664.
Condreay J.P., Witherspoon S.M., Clay W.C., Kost T.A. 1999. Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector. Proc. Natl. Acad. Sci. USA. 96, 127–132.
Hu YC. 2005. Baculovirus as a highly efficient expression vector in insect and mammalian cells. Acta Pharmacol. Sinica. 26, 405–416.
Kost TA., Condreay J.P. 1999. Recombinant baculovi-ruses as expression vectors for insect and mammalian cells. Curr. Opin. Biotechnol. 10, 428–433.
Van Loo N.D., Fortunati E., Ehlert E., Rabelink M., Grosveld F., Scholte B.J. 2001. Baculovirus infection of nondividing mammalian cells: Mechanisms of entry and nuclear transport of capsids. J. Virol. 75, 961–970.
Chuang C.-K., Sung L.-Y, Hwang S.-M., Lo W.-H., Chen H.-C., Hu Y.-C. 2007. Baculovirus as a new gene delivery vector for steam cells engineering and bone tissue engineering. Gene Ther. 14, 1417–1424.
Chen H.-C., Chang Y.-H., Chuang C.-K., Lin C.-Y, Sung L.-Y, Wang Y-H., Hu Y.-C. 2009. The repair of osteohondral defects using baculovirus-mediated gene transfer with de-differentiated chondrocytes in biore-actor culture. Biomaterials. 30, 674–681.
Fipaldini C., Bellei B., La Monica N. 1999. Expression of hepatitis c virus cDNA in human hepatoma cell line mediated by a hybrid baculovirus-HCV vector. Virology. 255, 302–311.
Reed K.E., Rice C.M. 2000. Overview of hepatitis C virus genome structure, polyprotein processing, and protein properties. Curr. Top. Microbiol. Immunol. 242, 55–84.
Baumert T.F., Ito S., Wong D.T., Liang T.J. 1998. Hepatitis C virus structural proteins assemble into viruslike particles in insect cells. J. Virol. 72, 3827–3836.
Ivanov A.V., Kuzyakin A.O., Kochetkov S.N. 2005. Molecular biology of hepatitis C virus. Usp. Biokhimii. 45, 37–86.
Maniatis T., Fritsch E.F., Sambrook J. 1982. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Lab. Press.
Bac-to-Bac Baculovirus Expression System. Instruction Manual. 1993. St. Louis, MO: Life Technologies Inc., Monsanto Corp. Res.