Bacteriocin biosynthesis contributes to the anti-inflammatory capacities of probiotic Lactobacillus plantarum

Beneficial microbes - Tập 9 Số 2 - Trang 333-344 - 2018
Xiangtian Yin1,2, Dustin Heeney1, Yanin Srisengfa1, Benjamin L. Golomb3,1, Stephen M. Griffey4, Maria L. Marco1
1Department of Food Science and Technology, University of California, One Shields Avenue, Davis, CA 95616 USA
2Department of Plant Pathology, University of California, One Shields Avenue, Davis, CA, 95616-8751, USA
3Bayer U.S. LLC, Crop Science Division, 890 Embarcadero Dr, West Sacramento, CA 95605, USA.
4Comparative Pathology Laboratory, School of Veterinary Medicine, University of California, 944 Garrod Dr. 2045 Davis, CA 95616, USA.

Tóm tắt

Plantaricin EF (PlnEF) is a class IIb bacteriocin produced by Lactobacillus plantarum. We compared L. plantarum NCIMB8826 and LM0419, a plnEFI deletion mutant of that strain lacking plnEF and the gene for the cognate immunity protein plnI, in a 2,4,6-trinitrobenzenesulfonic acid (TNBS) induced mouse model of acute inflammatory bowel disease. Mice fed either L. plantarum NCIMB8826 or LM0419 were not protected against TNBS according to either disease activity or histology (Ameho) scores. Mice consuming NCIMB8826 exhibited intermediate (non-significant) levels of colonic tumour necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) that ranged between the TNBS-treated animals and healthy controls. By comparison, TNF-α and IL-6 quantities were elevated in mice given L. plantarum LM0419 and equivalent to mice given TNBS alone. Both strains survived digestive tract transit in equal numbers and did not result in global changes to the bacterial composition in the intestine according to 16S rRNA gene sequencing either prior to or after TNBS administration. Examination of intestinal taxa showed that mice consuming wild-type L. plantarum, but not LM0419 contained lower proportions of Mucispirillum (Deferribacteres phylum) in the faeces prior to TNBS administration and Parabacteroides (Bacteroidetes phylum) in the caecum after disease induction. Parabacteroides also positively correlated with disease activity and histology scores. These findings suggest a role for PlnEFI production by L. plantarum in benefiting digestive tract health.

Từ khóa


Tài liệu tham khảo

Internal Medicine Journal, 47, 69

10.1007/s00253-016-7343-9

10.1136/gut.41.4.487

Austral Ecology, 26, 32

10.1016/j.anaerobe.2010.01.001

10.1080/10408398.2012.729231

10.1371/journal.pone.0037971

10.1128/JB.186.17.5721-5729.2004

10.1038/nmeth.f.303

10.4161/bbug.1.6.13642

10.1038/nature11319

10.1073/pnas.0700440104

Microbiology, 3, 777

10.1128/AEM.03006-05

10.1016/j.peptides.2009.05.014

PLoS ONE, 11, e0146162, 10.1371/journal.pone.0146162

10.1016/S1473-3099(07)70211-6

10.1073/pnas.1000162107

10.1007/s10620-006-3143-x

10.1038/nprot.2007.132

PLoS Pathogens, 10, 1

10.1152/ajpgi.00327.2009

10.1016/j.syapm.2005.06.001

10.1038/nature15524

10.1016/j.intimp.2015.02.026

Journal of Microbiology and Biotechnology, 19, 616

10.1128/AEM.01360-15

10.1128/AEM.71.12.8228-8235.2005

10.1128/AEM.01475-06

10.1002/ibd.22849

10.1371/journal.pone.0010632

Journal of Bacteriology, 181, 4848, 10.1128/JB.181.16.4848-4852.1999

10.1136/gutjnl-2011-300705

10.1097/00042737-200110000-00004

10.1111/j.1572-0241.2000.02015.x

10.1159/000104750

10.1007/s11274-014-1708-y

Clinical and Diagnostic Laboratory Immunology, 10, 696, 10.1128/CDLI.10.4.696-701.2003

Journal of Immunology, 158, 2099, 10.4049/jimmunol.158.5.2099

10.1016/j.resmic.2008.06.002

10.1038/oby.2011.111

10.1371/journal.pone.0031113

International Journal of Systematic and Evolutionary Microbiology, 55, 1199, 10.1099/ijs.0.63472-0

10.1002/ibd.20961

10.1002/mnfr.201600240

10.1128/AEM.70.5.2791-2800.2004

10.1073/pnas.1606290113

Current Protocols in Immunology, 49, 15.19.11

Frontiers in Immunology, 6, 96

10.1186/gb-2011-12-6-r60

10.1136/gutjnl-2011-301012

10.1111/1462-2920.12297

10.1159/000369711

PLoS ONE, 11, e0164036, 10.1371/journal.pone.0164036

10.1073/pnas.0809919106

10.1186/1471-2180-10-293

10.1186/s12866-015-0515-4

10.3168/jds.2013-7477

10.1111/1462-2920.12662

Thông tin
Thông tin xuất bản

Beneficial microbes

Tập 9 Số 2

333-344

Thông tin tác giả