Bacterial reduction and persistence after endodontic treatment procedures

Wiley - Tập 22 Số 1 - Trang 19-23 - 2007
Mitsuo Sakamoto1, José F. Siqueira, Isabela N. Rôças, Yoshimi Benno
1Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama, Japan

Tóm tắt

Bacteria that persist after endodontic disinfection procedures may lead to treatment failure. Over 50% of the bacteria found in endodontic infections are as‐yet‐uncultivated so investigations of bacteria that endure treatment procedures should include techniques that side‐step cultivation. This culture‐independent study evaluated the bacterial reduction promoted by intracanal disinfection procedures and identified the taxa persisting after treatment. Samples taken from the infected canals of teeth with apical periodontitis before treatment (S1), after instrumentation using NaOCl as irrigant (S2) and after interappointment medication with a calcium hydroxide paste (S3) were subjected to 16S rRNA gene clone library and real‐time polymerase chain reaction analyses. The S2 and S3 samples from five of the 15 canals showed negative results. In the other cases, instrumentation and instrumentation/medication promoted a significant reduction (99.67% and 99.85%, respectively) in the number of bacteria when compared to S1 samples. Forty‐three distinct bacterial taxa were identified, of which 24 (56%) were as‐yet‐uncultivated phylotypes. Nineteen of these 43 taxa (including eight as‐yet‐uncultivated phylotypes) were disclosed in post‐treatment samples, with streptococci being the most prevalent taxa. Findings demonstrated that culture‐independent methods provide a detailed insight into the effects of intracanal disinfection protocols, helping to define more effective strategies to deal with endodontic bacteria, including as‐yet‐uncultivated phylotypes.

Từ khóa


Tài liệu tham khảo

10.1111/j.1399-302X.1996.tb00180.x

10.1016/0030-4220(83)90333-X

10.1111/j.1365-2591.1985.tb00416.x

10.1046/j.1365-2591.2003.00686.x

10.1016/j.tripleo.2004.10.008

10.1016/j.joen.2005.10.014

10.1093/nar/gkg039

10.1128/AEM.61.7.2798-2801.1995

10.1097/01.don.0000152899.54247.4e

10.1111/j.1365-2591.1996.tb01375.x

10.1128/JCM.43.10.5332-5337.2005

10.1007/s00248-007-9320-4

10.1101/gr.3.3.181

10.1097/01.don.0000145035.85272.7c

10.1177/0810761

10.1099/00221287-148-1-257

10.1073/pnas.85.8.2444

10.1046/j.0143-2885.2001.00447.x

10.1099/13500872-142-8-2087

10.1097/00004770-200405000-00004

10.1099/jmm.0.45576-0

10.1111/j.1399-302X.2006.00270.x

10.1097/00004770-200012000-00022

Siqueira JF, 2001, Strategies to treat infected root canals, J Calif Dent Assoc, 29, 825, 10.1080/19424396.2001.12223244

10.1046/j.1365-2591.1999.00275.x

10.1097/01.don.0000157989.44949.26

10.1097/01.don.0000157990.86638.49

10.1016/S0099-2399(98)80151-9

10.1097/00004770-200206000-00010

10.1111/j.1600-0722.2004.00144.x

10.1046/j.1365-2591.1997.00092.x

10.1111/j.1365-2591.1991.tb00117.x

Sundqvist G, 1998, Essential endodontology, 242

10.1097/01.don.0000164856.27920.85

10.1073/pnas.162359199

Thông tin
Thông tin xuất bản

Wiley

Tập 22 Số 1

19-23

Thông tin tác giả