Bacillus pumilus Cyanide Dihydratase Mutants with Higher Catalytic Activity

Mary Abou-Nader Crum1, B.T. Sewell2, Michael J. Benedik1
1Department of Biology, Texas A & M University, College Station, TX, USA.
2Structural Biology Research Unit, Department of Integrative Biomedical Sciences, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa

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Abou Nader, 2012, Directed Evolution of Cyanide Degrading Enzymes.

Abou-Nader, 2010, Rapid generation of random mutant libraries., Bioeng. Bugs, 1, 337, 10.4161/bbug.1.5.12942

Basile, 2008, Genome mining of cyanide-degrading nitrilases from filamentous fungi., Appl. Microbiol. Biotechnol., 80, 427, 10.1007/s00253-008-1559-2

Baxter, 2006, The current and future applications of microorganism in the bioremediation of cyanide contamination., Antonie Van Leeuwenhoek, 90, 1, 10.1007/s10482-006-9057-y

Crum, 2015a, Probing C-terminal interactions of the Pseudomonas stutzeri cyanide-degrading CynD protein., Appl. Microbiol. Biotechnol., 99, 3093, 10.1007/s00253-014-6335-x

Crum, 2015b, C-terminal hybrid mutant of Bacillus pumilus cyanide dihydratase dramatically enhances thermal stability and pH tolerance by reinforcing oligomerization., J. Appl. Microbiol., 118, 881, 10.1111/jam.12754

DeSantis, 2003, Creation of a productive, highly enantioselective nitrilase through gene site saturation mutagenesis (GSSM)., J. Am. Chem. Soc., 125, 11476, 10.1021/ja035742h

Devuyst, 1989, A cyanide removal process using sulfur dioxide and air., JOM, 41, 43, 10.1007/BF03220847

Fisher, 1952, Colorimetric determination of cyanide in stack gas and waste water., Anal. Chem., 24, 1440, 10.1021/Ac60069a014

Jaeger, 2004, Enantioselective biocatalysis optimized by directed evolution., Curr. Opin. Biotechnol., 15, 305, 10.1016/j.copbio.2004.06.007

Jandhyala, 2003, CynD, the cyanide dihydratase from Bacillus pumilus: gene cloning and structural studies., Appl. Environ. Microbiol., 69, 4794, 10.1128/AEM.69.8.4794-4805.2003

Jandhyala, 2005, Comparison of cyanide-degrading nitrilases., Appl. Microbiol. Biotechnol., 68, 327, 10.1007/s00253-005-1903-8

Knorre, 1984, “Cyanide detoxification with hydrogen peroxide using the Degussa process,” in, Proceedings of the Cyanide and the Environment Conference

Kumaran, 2003, Crystal structure of a putative CN hydrolase from yeast., Proteins, 52, 283, 10.1002/Prot.10417

La Brooy, 1994, Review of gold extraction from ores., Miner. Eng., 7, 1213, 10.1016/0892-6875(94)90114-7

Meyers, 1993, Isolation and characterization of a cyanide dihydratase from Bacillus pumilus C1., J. Bacteriol., 175, 6105, 10.1128/jb.175.19.6105-6112.1993

Nichols, 1934, Reactions of Nessler’s solution., J. Am. Chem. Soc., 56, 769, 10.1021/ja01319a003

Sali, 1993, Comparative protein modeling by satisfaction of spatial restraints., J. Mol. Biol., 234, 779, 10.1006/jmbi.1993.1626

Sewell, 2005, Oligomeric structure of nitrilases: effect of mutating interfacial residues on activity., Ann. N. Y. Acad. Sci., 1056, 153, 10.1196/annals.1352.025

Thuku, 2009, Microbial nitrilases: versatile, spiral forming, industrial enzymes., J. Appl. Microbiol., 106, 703, 10.1111/j.1365-2672.2008.03941.x

Thuku, 2007, Post-translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form., FEBS J., 274, 2099, 10.1111/j.1742-4658.2007.05752.x

Wang, 2012, Engineering pH-tolerant mutants of a cyanide dihydratase., Appl. Microbiol. Biotechnol., 94, 131, 10.1007/s00253-011-3620-9

Watanabe, 1998, Cyanide hydrolysis in a cyanide-degrading bacterium, Pseudomonas stutzeri AK61, by cyanidase., Microbiology, 144(Pt 6), 1677, 10.1099/00221287-144-6-1677

Zhang, 2014, Structural insights into enzymatic activity and substrate specificity determination by a single amino acid in nitrilase from Synechocystis sp PCC6803., J. Struct. Biol., 188, 93, 10.1016/j.jsb.2014.10.003