Bacillus globigii cell size is influenced by variants of the quorum sensing peptide extracellular death factor
Tóm tắt
Toxin-antitoxin modules are necessary for the mode of action of several antibiotics. One of the most studied toxin-antitoxin modules is the quorum sensing—dependent MazEF system in Escherichia coli. The quorum sensing factor in this system is called the extracellular death factor (EDF), a linear pentapeptide with the sequence NNWNN. In spite of the extensive research on the mazEF system and the involvement of the quorum sensing factor EDF, the effect of EDF itself on bacteria has not yet been studied. In this research, we determined the effect of EDF and variants on cell growth in the Gram-negative bacterium E. coli and the Gram-positive Bacillus globigii. By aligning the zwf gene (from where EDF originates) of different bacterial species, we found 27 new theoretical variants of the peptide. By evaluating growth curves and light microscopy we found that three EDF variants reduced bacterial cell size in B. globigii, but not in E. coli. The D-peptides did not affect cell size, indicating that the effect is stereospecific. Peptides wherein tryptophan was substituted by alanine also did not affect cell size, which indicates that the effect seen is mediated by an intracellular target.
Tài liệu tham khảo
Aizenman E, Engelberg-Kulka H, Glaser G (1996) An Escherichia coli chromosomal “addiction module” regulated by guanosine (corrected) 3′,5′-bispyrophosphate: a model for programmed bacterial cell death. Proc Natl Acad Sci USA 93(12):6059–6063
Amitai S, Yassin Y, Engelberg-Kulka H (2004) MazF-mediated cell death in Escherichia coli: a point of no return. J Bacteriol 186(24):8295–8300. doi:10.1128/JB.186.24.8295-8300.2004
Belitsky M, Avshalom H, Erental A, Yelin I, Kumar S, London N, Sperber M, Schueler-Furman O, Engelberg-Kulka H (2011) The Escherichia coli extracellular death factor EDF induces the endoribonucleolytic activities of the toxins MazF and ChpBK. Mol Cell 41(6):625–635. doi:10.1016/j.molcel.2011.02.023
Chien AC, Hill NS, Levin PA (2012) Cell size control in bacteria. Curr Biol 22(9):R340–R349. doi:10.1016/j.cub.2012.02.032
Fico S, Mahillon J (2006) TasA-tasB, a new putative toxin-antitoxin (TA) system from Bacillus thuringiensis pGI1 plasmid is a widely distributed composite mazE-doc TA system. BMC Genomics 7:259. doi:10.1186/1471-2164-7-259
Florek P, Levdikov VM, Blagova E, Lebedev AA, Skrabana R, Resetarova S, Pavelcikova P, Barak I, Wilkinson AJ (2011) The structure and interactions of SpoIISA and SpoIISB, a toxin-antitoxin system in Bacillus subtilis. J Biol Chem 286(8):6808–6819. doi:10.1074/jbc.M110.172429
Hayes F (2003) Toxins-antitoxins: plasmid maintenance, programmed cell death, and cell cycle arrest. Science 301(5639):1496–1499. doi:10.1126/science.1088157301/5639/1496
Kamada K, Hanaoka F, Burley SK (2003) Crystal structure of the MazE/MazF complex: molecular bases of antidote-toxin recognition. Mol Cell 11(4):875–884
Kolodkin-Gal I, Engelberg-Kulka H (2008) The extracellular death factor: physiological and genetic factors influencing its production and response in Escherichia coli. J Bacteriol 190(9):3169–3175. doi:10.1128/JB.01918-07
Kolodkin-Gal I, Hazan R, Gaathon A, Carmeli S, Engelberg-Kulka H (2007) A linear pentapeptide is a quorum-sensing factor required for mazEF-mediated cell death in Escherichia coli. Science 318(5850):652–655. doi:10.1126/science.1147248
Lepka D, Kerrinnes T, Skiebe E, Hahn B, Fruth A, Wilharm G (2009) Adding to Yersinia enterocolitica gene pool diversity: two cryptic plasmids from a biotype 1A isolate. J Biomed Biotechnol 2009:398434. doi:10.1155/2009/398434
Liu X, Zhu S, Ye W, Ruan L, Yu Z, Zhao C, Sun M (2008) Genetic characterization of two putative toxin-antitoxin systems on cryptic plasmids from Bacillus thuringiensis strain YBT-1520. J Microbiol Biotechnol 18(10):1630–1633
Pedersen K, Christensen SK, Gerdes K (2002) Rapid induction and reversal of a bacteriostatic condition by controlled expression of toxins and antitoxins. Mol Microbiol 45(2):501–510
Sat B, Hazan R, Fisher T, Khaner H, Glaser G, Engelberg-Kulka H (2001) Programmed cell death in Escherichia coli: some antibiotics can trigger mazEF lethality. J Bacteriol 183(6):2041–2045
Trueba FJ, Woldringh CL (1980) Changes in cell diameter during the division cycle of Escherichia coli. J Bacteriol 142(3):869–878
Veerman EC, Valentijn-Benz M, Nazmi K, Ruissen AL, Walgreen-Weterings E, van Marle J, Doust AB, van’t Hof W, Bolscher JG, Amerongen AV (2007) Energy depletion protects Candida albicans against antimicrobial peptides by rigidifying its cell membrane. J Biol Chem 282(26):18831–18841. doi:10.1074/jbc.M610555200
Weart RB, Lee AH, Chien AC, Haeusser DP, Hill NS, Levin PA (2007) A metabolic sensor governing cell size in bacteria. Cell 130(2):335–347. doi:10.1016/j.cell.2007.05.043
Yau WM, Wimley WC, Gawrisch K, White SH (1998) The preference of tryptophan for membrane interfaces. Biochemistry 37(42):14713–14718. doi:10.1021/bi980809cbi980809c
Zhang J, Zhang Y, Inouye M (2003) Characterization of the interactions within the mazEF addiction module of Escherichia coli. J Biol Chem 278(34):32300–32306. doi:10.1074/jbc.M304767200M304767200