Azospirillum brasilense glutamine synthetase: influence of the activating metal ions on the enzyme properties
Tóm tắt
The kinetic properties of the Mg2+-activated and Mn2+-activated glutamine synthetase (GS) of Azospirillum brasilense in the biosynthetic reaction were studied. The Mg2+-supported and Mn2+-supported GSs in an average state of adenylylation varied in pH optimum, maximum activity, saturation functions for ammonium and glutamate, affinity to substrates, and in the Me2+-ATP ratio required for the optimal enzyme activity. Seventeen other cations were tested for the maintenance of GS activity. The level of the latter and the kinetic behavior of the GS in A.brasilense is suggested to depend essentially on the concentrations of Mg2+, Mn2+ and Co2+, as well as on their ratio
Tài liệu tham khảo
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