Auxin‐induced fruit‐set in tomato is mediated in part by gibberellins

Plant Journal - Tập 56 Số 6 - Trang 922-934 - 2008
Juan Carlos Serrani1,2, Omar Ruíz‐Rivero1,2, Mariano Fos3, José L. García‐Martínez1
1Instituto de Biología Molecular y Celular de Plantas (Universidad Politécnica de Valencia-CSIC)
2these authors contributed equally to this work
3Departamento de Biología Vegetal, Universidad Politécnica de Valencia, 46022-Valencia, Spain

Tóm tắt

SummaryTomato (Solanum lycopersicumL.) fruit‐set and growth depend on gibberellins (GAs). Auxins, another kind of hormone, can also induce parthenocarpic fruit growth in tomato, although their possible interaction with GAs is unknown. We showed that fruit development induced by the auxins indole‐3‐acetic acid and 2,4‐dichlorophenoxyacetic acid (2,4‐D) were significantly reduced by the simultaneous application of inhibitors of GA biosynthesis, and that this effect was reversed by the application of GA3. This suggested that the effect of auxin was mediated by GA. Parthenocarpic fruits induced by 2,4‐D had higher levels of the active GA1, its precursors and metabolites, than unpollinated non‐treated ovaries, but similar levels as those found in pollinated ovaries. Application experiments of radioactive‐labelled GAs to unpollinated ovaries showed than 2,4‐D altered GA metabolism (both biosynthesis and catabolism)in vivo. Transcript levels of genes encoding copalyldiphosphate synthase (SlCPS), SlGA20ox1, SlGA20ox2 and SlGA20ox3, and SlGA3ox1 were higher in unpollinated ovaries treated with 2,4‐D. In contrast, transcript levels ofSlGA2ox2(out of the fiveSlGA2oxgenes known to encode this kind of GA‐inactivating enzyme) were lower in ovaries treated with 2,4‐D. Our results support the idea that auxins induce fruit‐set and growth in tomato, at least partially, by enhancing GA biosynthesis (GA 20‐oxidase, GA 3‐oxidase and CPS), and probably by decreasing GA inactivation (GA2ox2) activity, thereby leading to higher levels of GA1. The expression of diverse Aux/indole‐3‐acetic acid (IAA) and auxin response factors, which may be involved in this effect of auxin, was also altered in 2,4‐D‐induced ovaries.

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Plant Journal

Tập 56 Số 6

922-934

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