Autophagy and proinflammatory cytokine expression in the intestinal mucosa and mesenteric fat tissue of patients with Crohn's disease

Journal of Coloproctology - Tập 33 - Trang 9-15 - 2013
Raquel Franco Leal1,2,3, Marciane Milanski2, Cláudio Saddy Rodrigues Coy1,3, Mariana Portovedo2, Viviane Soares Rodrigues1,2, Andressa Coope2, Maria de Lourdes Setsuko Ayrizono1,3, João José Fagundes1,3, Lício Augusto Velloso2
1Coloproctology Unit, Surgery Department, Escola de Ciências Médicas, Universidade Estadual de Campinas (UNICAMP), São Paulo, SP, Brazil
2Laboratory of Cell Signaling, Internal Medicine Department, Escola de Ciências Médicas, UNICAMP, São Paulo, SP, Brazil
3Brazilian Society of Coloproctology (TSBC), Rio de Janeiro, RJ, Brazil

Tóm tắt

Abstract Background Recently, mesenteric fat has been proposed to play a role in the pathophysiol- ogy of Crohn's disease (CD), as fat hypertrophy is detected close to the affected intestinal area; however, there are few studies regarding autophagy and creeping fat tissue in CD. Objective Evaluate autophagy-related proteins and proinflammatory cytokines in intestinal mucosa and mesenteric fat in patients with CD and controls. Patients and methods Ten patients with CD, eight with non-inflammatory disease who underwent surgery, and eight with normal ileocolonoscopy were studied. The expression of LC3-II, TNF-α and IL-23 was determined by immunoblot of protein extracts. In addition, total RNA of LC3 and Atg16-L1 were determined using RT-PCR. Results The expression of LC3-II was significantly lower in the mesenteric tissue of CD when compared to controls (p < 0.05). In contrast, the intestinal mucosa of the CD group had higher levels of LC3-II (p < 0.05). However, mRNA expression of autophagy-related pro- teins was similar when compared to mesenteric fat groups. TNF-α and IL-23 expressions were higher in intestinal mucosa of CD than in control (p < 0.05). Conclusion These findings suggest a defect in the autophagic activity of the creeping fat tissue in CD, which could be involved with the maintenance of the inflammatory process in the intestinal mucosa.

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