Autologous bone marrow‐derived cells with placental extract for healing excisional cutaneous wounds in animal model

International Wound Journal - Tập 10 Số 2 - Trang 167-176 - 2013
Ashok Akela1, Samit Kumar Nandi2, Partha Das3, Dibyajyoti Banerjee4, Subhasis Roy5, Uttam Datta6
1A Akela, MVSc, Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, Kolkata 700037, India
2SK Nandi, PhD, Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, Kolkata 700037, India
3P Das, PhD, Department of Veterinary Anatomy, West Bengal University of Animal and Fishery Sciences, Kolkata 700037, India
4D Banerjee, MD, Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
5S Roy, PhD, KrishiVigyan Kendra, ICAR, Nimpith, India
6U Datta, PhD, Department of Veterinary Gynecology, West Bengal University of Animal and Fishery Sciences, Kolkata 700037, India

Tóm tắt

Topical wound‐healing potential of autologous bone marrow‐derived nucleated cells along with placental extract was evaluated in comparison with buffy coat of autologous blood on full‐thickness cutaneous wounds in the thoracolumbar region of 15 clinically healthy New Zealand rabbits. Three wounds of 2 × 2 cm, one on the right side of the body and two on the left side of the midline were created on the dorsal lumbar region of each rabbit under xylazine–ketamine anaesthesia. The wounds of each animal were randomly assigned to one of the three treatments: topical application of autologous bone marrow‐derived cells with placental extract (group I), application of buffy coat in the autologous plasma with placental extract (group II) and autologous plasma with placental extract as control (group III). Wounds were observed for 30 days macroscopically and for granulation tissue formation, histomorphological and histochemical evaluation. Time of appearance of granulation tissues and filling of wound beds were faster in group I followed by group II and group III animals, respectively. Histomorphological findings exhibited an earlier disappearance of inflammatory reaction, better epithelialisation, significantly maximum neovascularisation, fibroplasias and collagenation in group I followed by group II and group III animals, respectively. Histochemical findings also depicted maximum number of robust, thick, interwoven type of collagen fibres, stout, highly tortuous and interwoven network of elastin fibres and numerous mesh war form of reticulin fibres within the dermal component were present in group I when compared with group II and III animals. Experiment conclude that single application of autologous bone marrow‐nucleated cells with placental extract topically could be a novel option for faster healing in complicated non healing wounds both in human beings and animals.

Từ khóa


Tài liệu tham khảo

10.1533/9781845695545.1.25

Deodhar AK, 1997, Surgical physiology of wound healing: a review, J Postgrad Med, 43, 52

Lee AH, 1984, Effects of gentamicin solution and cream on the healing of open wound, Am J Vet Res, 45, 1487

10.1146/annurev.bi.52.070183.003553

Swaim SF, 2001, Wound contraction: basic and clinical factors, Compendium, 23, 20

Layton CE., 1993, Surgical complications and wound healing in the small animal practice, 89

10.1056/NEJM199909023411006

Harari J., 1993, Surgical complication and wound healing in the small animal practice, 78

Lee AH, 1987, Effects of non‐adherent dressing materials on the healing of open wound in dogs, J Am Vet Med Assoc, 190, 416

10.1007/s00580-006-0616-4

10.1002/jor.1100090504

10.1016/j.jss.2006.07.037

Neagos D, 2006, Skin wound healing in a free floating fibroblast populated collagen lattice model, Rom J Biophys, 16, 157

10.1016/S0002-9440(10)63482-7

10.1634/stemcells.22-5-812

10.1111/j.1365-2133.2005.06554.x

10.1089/ten.2005.11.110

10.12968/jowc.2006.15.7.26937

Kahn CM., 2005, Merck veterinary manual, 149

Crow SE, 1997, Manual of clinical procedures in the dog, cat and rabbit, 169

Kasten P, 2007, Instant mesenchymal stem cell therapy: how can we do it? Characterization and concentration of mesenchymal stem cells in vitro, Orthop Surg, European cells Mater, 14, 39.

Bigbie RB, 1991, Effect of amnion and yeast cell derivate on second intention healing in horses, Am J Vet Res, 52, 1376., 10.2460/ajvr.1991.52.08.1376

Masson P., 1929, Trichrome staining and their preliminary techniques, J Tech Methods, 12, 75

10.1093/ajcp/21.9_ts.897

Mallony FB., 1961, Pathological techniques, 152.

Swaim SF, 1990, Small animal wound management, 1

Fossum TW, 2007, Manual of small animal surgery, 132

Kumar A., 1996, Veterinary surgical techniques, 151

Borena B, 2009, Evaluation of healing potential of autologous bone marrow‐derived nucleated cells on incisional wounds in dogs, Indian J Vet Surg, 30, 85

10.1016/j.biocel.2003.12.003

Singh SDJ, 2005, Wound healing activity of the leaf extracts and de‐oxyelephantopin isolated from Elephantopus scaber Linn, Indian J Pharmacol, 37, 237

10.1002/jcp.10260

10.1001/archderm.139.4.510

10.1111/j.1365-3164.2004.00410_4-2.x

Nelson DL, 2000, Lehninger principles of biochemistry

Biswas TK, 2001, Wound healing activity of human placental extracts in rat, Acta Pharmacol Sin, 22, 1113

10.1001/archderm.1994.01690040093015

10.1046/j.1365-2133.2003.05164.x

10.1016/j.jchromb.2004.09.059

10.1159/000244506

10.1016/j.jss.2004.04.008

10.1089/ten.2006.12.2707

Thông tin
Thông tin xuất bản

International Wound Journal

Tập 10 Số 2

167-176

Thông tin tác giả