Asymbiotic in vitro seed propagation of Dendrobium

Jaime A. Teixeira da Silva1, Е. А. Цавкелова2, Tzi Bun Ng3, S. Parthibhan4, Judit Dobránszki5, Jean Carlos Cardoso6, M. S. Rao4, Songjun Zeng7
1P.O. Box 7, Miki-cho Post Office, 3011-2, Ikenobe, Kagawa 761-0799, Japan
2Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia
3School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China
4Department of Plant Science, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India
5Research Institute of Nyíregyháza, University of Debrecen, Nyíregyháza, Hungary
6Department of Rural Development, Centro de Ciências Agrárias, UFSCar, Via Anhanguera, km 174, CP 153, CEP 13.600-970, Araras City, Brazil
7Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China

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Alam MK, Rashid MH, Hossain MS, Salam MA, Rouf MA (2002) In vitro seed propagation of dendrobium (Dendrobium transparens) orchid as influenced by different media. Biotechnol 1:111–115

Ali MJ, Murdad R, Latip MA (2011) In vitro seed germination of Bornean endemic orchids Dendrobium tetrachromum and Dendrobium hamaticalcar. Empower Sci Tech Innov: Towards Better Tomorrow, Proceedings of UMTAS 2011. Universiti Malaysia Sabah, Malaysia, pp 770–778

Alvarez-Pardo VM, Ferreira AG, Nunes VF (2006) Seed disinfestation methods for in vitro cultivation of epiphyte orchids from Southern Brazil. Hortic Bras 24:217–220

Arditti J (1967) Factors affecting the germination of orchid seeds. Bot Rev 33:1–97

Arditti J (1979) Aspects of the physiology of orchids. Adv Bot Res 7:421–655

Atichart P, Bunnag S, Theerakulpisut P (2007) Agrobacterium-mediated transformation of Dendrobium secundum (Bl.) Lindl. with antisense ACC oxidase. Asian J Plant Sci 6:1065–1071

Bernard N (1909) L’evolution dans la symbiose, les Orchidées et leur champignons commensaux. Ann Sci Nat Bot 9:1–196

Buffa Filho W, Pereira AMS, França SC, Furla-ni M (2002) Indução de metabólitos bioativos em culturas de células de Maytenus ilicifolia. Eclética Química 27:403–416 (in Portuguese with English abstract)

Bulpitt CJ (2005) The uses and misuses of orchids in medicine. Q J Med 98:625–631

Bulpitt CJ, Li Y, Bulpitt PF, Wang J (2007) The use of orchids in Chinese medicine. J R Soc Med 100:558–563

Campos DM (2002) Orquídeas: manual prático de cultura. 3rd edn. Expressão e Cultura, Rio de Janeiro, p 143 (in Portuguese)

Cardoso JC (2012) Dendrobium ‘Brazilian Fire 101’—new option of color of flowers for the orchid market. Hortic Bras 30:561–564

Chattopadhyay P, Banerjee N, Chaudhary B (2012) Genetic characterization of selected medicinal Dendrobium (Orchidaceae) species using molecular markers. Res J Biol 2:117–125

Chen ZL, Zeng SJ, Duan J (2004) In vitro propagation of Dendrobium unicum. Plant Physiol Commun 40:342 (in Chinese with English abstract)

Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sin 18:659–668

Chugh S, Guha S, Rao IU (2009) Micropropagation of orchids: a review on the potential of different explants. Sci Hortic 122:507–520

Cui QH, Sun YY, Duan ML, Li K, Huang CX, Yan H (2012) Optimization of sterile germination condition for Dendrobium devonianum. J Northwest Forest Univ 27:106–110 (in Chinese with English abstract)

Curtis J (1936) For native orchids in culture. Am Orchid Soc Bull 5:42–47

de Moraes CP, Diogo JA, Canabrava RI, Pedro NP, Furtado ALFF, Marteline MA (2010) Desenvolvimento in vitro de Dendrobium nobile Lind. (Orchidaceae) em recipientes de diferentes volumes. R Bras Bioci Porto Alegre 8(2):225–228 (in Portuguese with English abstract)

Ding CC (2004) Study on tissue culture and rapid propagation technic of Dendrobium devonianum. Trop Agric Sci Technol 27:10–11 (in Chinese with English abstract)

Dohling S, Kumaria S, Tandon P (2008) Optimization of nutrient requirement for asymbiotic seed germination of Dendrobium longicornu Lindl. and D. formosum Roxb. Proc Indian Natl Sci Acad 74(4):167–171

Dressler RL (2005) How many orchid species? Selbyana 26:155–158

Du G, Yang HY, Zhu SL, Zhang GQ (2007) Experiment on seeding induction from seed of Dendrobium candidum. J Chin Med Mater 30:1207–1208 (in Chinese with English abstract)

Du G, Lai TC, Yang HY (2012) Study on the tissue culture of Dendrobium aphyllum (Roxb) C. E. C. Fisch. North Hort 8:140–141 (in Chinese with English abstract)

Dutta S, Chowdhurry A, Bhattacharjee B, Nath PK, Dutta BK (2011) In vitro multiplication and protocorm development of Dendrobium aphyllum (Roxb.) CEC Fisher. Assam Univ J Sci Technol Biol Env Sci 7:57–62

Ernst R (1982) Paphiopedilum. In: Arditti J (ed) Orchid Biology: Reviews and Perspectives, vol II., Cornell University PressIthaca, New York, pp 350–353

Faria RT, Rodrigues FN, Oliveira LVR, Müller C (2004a) In vitro Dendrobium nobile plant growth and rooting in different sucrose concentrations. Hortic Bras 22:780–783 (in Portuguese with English abstract)

Faria RT, Vicente APRM, Costa TMM, Fonseca ICB, Silva GL, Takahashi LSA (2004b) Seleção de genótipos de Dendrobium (Orchidaceae) na fase de propagação in vitro. Semina: Ciências Agrárias 25:309–314 (in Portuguese with English abstract)

Fay MF, Chase MW (2009) Orchid biology: from Linnaeus via Darwin to the 21st century. Ann Bot 104:359–364

Ferreira WM, Suzuki RM, Pescador R, Figueiredo-Ribeiro RCL, Kerbauy G (2011) Propagation, growth, and carbohydrates of Dendrobium Second Love (Orchidaceae) in vitro as affected by sucrose, light, and dark. In Vitro Cell Dev Biol Plant 47:420–427

Fu CM, Zhao ZG, Huang NZ, He JX, Tang FL, Shi YP (2012) Study on technology of aseptic sowing and rapid propagation of Dendrobium officinale. Guihaia 32:238–242

Galdiano-Júnior RF, Neto PC, Mantovani C (2011) Crescimento in vitro de Dendrobium nobile Lindley com adição de carvão ativado. Científica 40:28–34 (in Portuguese with English abstract)

Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

Gong JY, Wang HX, Chen BL, Chen E, Li YX, Wang ZN (2015) Asymbiotic in vitro seed germination of three Dendrobium species and capsule characteristics observation. Jiangsu Agric Sci 43(1):184–186 (in Chinese)

Gu S, Ding XY, Wang Y, Zhou Q, Ding G, Li XX, Qian L (2007) Isolation and characterization of microsatellite of markers in Dendrobium officinale, an endangered herb endemic to China. Mol Ecol Notes 7:1166–1168

Guo S, Xu J (1990) Effects of fungi and its liquid extract on seed germination of Dendrobium hancockii Rolf. J Chin Mat Med 15(7397–7399):445 (in Chinese with English abstract)

Hajong S, Kumaria S, Tandon P (2010) In vitro propagation of the medicinal orchid Dendrobium chrysanthum. Proc Indian Natl Sci Acad 76:1–6

Harvais G (1972) The development and growth requirements of Dactylorhiza purpurella in asymbiotic culture. Can J Bot 50:1223

Hazarika RB, Sarma CM (1995) In vitro germination and regeneration of Dendrobium transparens Lindl. J Ochid Soc India 9:51–54

Hossain MM (2013) In vitro embryo morphogenesis and micropropagation of Dendrobium aggregatum Roxb. Plant Tissue Cult Biotech 23:241–249

Hossain MM, Kant R, Van PT, Winarto B, Zeng SJ, Teixeira da Silva JA (2013a) The application of biotechnology to orchids. Crit Rev Plant Sci 32:69–139

Hossain MM, Sharma M, Pathak P (2013b) In vitro propagation of Dendrobium aphyllum (Orchidaceae)—seed germination to flowering. J Plant Biochem Biotechnol 22:157–167

Hu Z, He JB (1979) Sterile germination and in vitro rapid propagation of Dendrobium tosaense. Plant J 3:6–7 (in Chinese)

Huo LL, Pan HT, Zhang QX (2010) Asepsis sowing and rapid propagation of Dendrobium pendulum Roxb. Plant Physiol Commun 46:1269–1270 (in Chinese)

Kabir MF, Rahman MS, Jamal A, Rahman M, Khalekuzzaman M (2013) Multiple shoot regeneration in Dendrobium fimbriatum hook an ornamental orchid. J Anim Plant Sci 23:1140–1145

Kaewduangta W, Reamkatog P (2011) Effect of modification medium on growth development of Dendrobium parishii in vitro. Am Eurasian J Agric Environ Sci 11:117–121

Kamemoto H, Kuehnle AR, Amore TD (1999) Breeding Dendrobium orchids in Hawaii. University of Hawaii Press, Honolulu

Kananont N, Pichyangkura R, Chanpramed S, Chadchawan S, Limpanavech P (2010) Chitosan specificity for the in vitro seed germination of two Dendrobium orchids (Asparagales: Orchidaceae). Sci Hortic 124:239–247

Kauth PJ, Dutra D, Johnson TR, Stewart SL, Kane ME, Vendrame W (2008) Techniques and applications of in vitro orchid seed germination. In: Teixeira da Silva JA (ed) Floriculture, Ornamental and Plant Biotechnology: advances and topical issues. 1st edn. vol V., Global Science Books, Ltd., Isleworth, Chapter 45, pp 375–391

Keel BG, Zettler LW, Kaplin BA (2011) Seed germination of Habenaria repens (Orchidaceae) in situ beyond its range, and its potential for assisted migration imposed by climate change. Castanea 76:43–54

Knop W (1865) Quantitative Untersuchungen uber die Ernahrungsprozesse der Pflanzen. Landwirtsch Vers Stn 7:93–107

Knudson L (1921) La germinación simbiótica de las semillas de orquideas. Bol Real Soc Española Hist Nat 21:250–260

Knudson L (1922) Nonsymbiotic germination of orchid seeds. Bot Gazette 73:1–25

Kong Q, Yuan SY, Gy Végvári (2007) Micropropogation of an orchid Dendrobium strongylanthum Rchb.f. Int J Hortic Sci 13:61–64

Kuehnle AR (2007) Orchids: Dendrobium. In: Anderson NO (ed) Flower breeding and genetics. Springer, pp. 539–560

Kumar K, Majumdar S, Sharma R, Sharma B (2006) Green pod culture and rapid micropropagation of Dendrobium chrysanthum Wall—a horticultural and medicinal orchid. Folia Hortic 18:81–90 (Retracted; http://www.degruyter.com/view/j/fhort.2013.25.issue-2/fhort-2013-0019/fhort-2013-0019.xml?format=INT )

Kumar P, Rawat GS, Wood HP (2011) Diversity and ecology of Dendrobiums (Orchidaceae) in Chotanagpur plateau, India. Taiwania 56:23–36

Lan YT, Liu SY, Luo YT, Huang L, Li ZL, Wei XL (2010) Sterile germination of Dendrobium chrysotoxum seeds and rapid propagation of its plantlets. Agric Sci Technol 11:89–91 (in Chinese with English abstract)

Li C, Zhang ZJ, Wei Y, Li LX, Ling ZZ, Wei KH (2013) Study on tissue culture and rapid propagation of Dendrobium fimbriatum Hook. var. oculatum Hook. North Hortic 6:105–107 (in Chinese with English abstract)

Lin Y, Li J, Li B, He T, Chun Z (2011) Effects of light quality on growth and development of protocorm-like bodies of Dendrobium officinale in vitro. Plant Cell Tiss Organ Cult 105:329–335

Liu SQ (2008) Tissue culture and rapid propagation of Dendrobium henryi. Subtrop Plant Sci 37(4):57–58 (in Chinese without English abstract)

Liu ZJ, Zhang YT, Wang Y, Huang QH, Chen XQ, Chen LQ (2011) Recent developments in the study of rapid propagation of Dendrobium catenatum Lindl. with a discussion on its scientific and Chinese names. Plant Sci J 29:763–772 (in Chinese with English abstract)

Lo SF, Nalawade SM, Kuo CL, Chen CL, Tsay HS (2004a) Asymbiotic germination of immature seeds, plantlet development and ex vitro establishment of plants of Dendrobium tosaense Makino—a medicinally important orchid. In Vitro Cell Dev Biol Plant 40:528–535

Lo SF, Nalawade SM, Mulabagal V, Mathew S, Chen CL, Kuo CL, Tsay HS (2004b) In vitro propagation by asymbiotic seed germination and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity studies of tissue culture raised plants of three medicinally important species of Dendrobium. Biol Pharm Bull 27:731–735

Lone AB, Barbosa CM, Faria RT, Takahashi LSA, Fonseca ICB (2008) Seleção de genótipos de Dendrobium phalaenopsis (Orchidaceae) nas fases de propagação in vitro e aclimatização. Semina: Ciências Agrárias 29:755–760 (in Portuguese with English abstract)

Luo JP, Wawrosch C, Kopp B (2009) Enhanced micropropagation of Dendrobium huoshanense C.Z. Tang et S.J. Cheng through protocorm-like bodies: the effects of cytokinins, carbohydrate sources and cold pretreatment. Sci Hortic 123:258–262

Luo XQ, Wu MK, Shen G, Zhang XB (2013) Guizhou Karst areas Dendrobium officinale re-introduction conservation and sustainable utilization. Chin Wild Plant Resour 32(6):47–50 (in Chinese with an English abstract)

Manz B, Müller K, Kucera B, Volke F, Leubner-Metzger G (2005) Water uptake and distribution in germinating tobacco seeds investigated in vivo by nuclear magnetic resonance imaging. Plant Physiol 138:1538–1551

McKendrick S (2000) In vitro germination of orchids: a manual. Ceiba Foundation for Tropical Conservation. www.ceiba.org/documents/CFTCpropman.doc . Accessed 12 June 2015

Meng Y, Liu CH, Kong LJ, Zhang XY, Yang BY (2012) Sterile seeding and rapid propagation of Dendrobium shixingense. J Nanchang Univ (Nat Sci) 36(4):385–388 (in Chinese with English abstract)

Mitra GC, Prasad RN, Choudhury RA (1976) Inorganic salts and differentiation of protocorms in seed callus of orchid correlative changes in its free amino acid content. Indian J Exp Biol 14:350–351

Mo ZZ, Ling ZH (2007) Effects of exogenous hormones on seed germination and protocorm multiplication of Dendrobium longicornu Lind. J Anhui Agri Sci 35:9568–9570 (in Chinese with English abstract)

Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

Mweetwa AM, Welbaum GE, Tay D (2008) Effects of development, temperature, and calcium hypochlorite treatment on in vitro germinability of Phalaenopsis seeds. Sci Hortic 117:257–262

Nambiar N, Tee CS, Maziah M (2012) Effects of organic additivies [sic] and different carbohydrate sources on proliferation of protocorm-like bodies in Dendrobium Alya Pink. Plant Omics J 5:10–18

Ng TB, Liu JY, Wong JH, Ye XJ, Sze SCW, Tong Y, Zhang KY (2012) Review of research on Dendrobium, a prized folk medicine. Appl Microbiol Biotechnol 93:1795–1803

Ngampanya B, Homlaaor W (2010) Simple media for Dendrobium orchid seed germination and protocorm development. Acta Hortic 878:219–224

Nimoto DH, Sagawa Y (1961) Ovule development in Dendrobium. Amer Orchid Soc Bull 30:813–819

Nongdam P, Tikendra L (2014) Establishment of an efficient in vitro regeneration protocol for rapid and mass propagation of Dendrobium chrysotoxum Lindl. using seed culture. Sci World J 2014:740150

Parthibhan S, Franklin BJH, Muthukumar M, Sherif NA, Senthil Kumar T, Rao MV (2012) Influence of nutritional media and photoperiods on in vitro asymbiotic seed germination and seedling development of Dendrobium aqueum Lindley. African J Plant Sci 6:383–393

Paul S, Kumaria S, Tandon P (2012) An effective nutrient medium for asymbiotic seed germination and large-scale in vitro regeneration of Dendrobium hookerianum, a threatened orchid of northeast India. AoB Plants 2012:plr032

Qian WL, Zhang JX, Wu KL, Zeng SJ (2013) Study on propagation and cultivation technique of Dendrobium huoshanense seedlings. J Trop Subtrop Bot 21:240–246 (in Chinese with English abstract)

Rasmussen HN (1995) Terrestrial Orchids: From Seed to Mycotrophic Plant. Cambridge University Press, Cambridge, p 460

Roberts DL, Dixon KW (2008) Orchids. Curr Biol 18:R325–R329

Saiprasad GVS, Raghuveer P, Khetarpal S, Chandra R (2004) Effect of various polyamines on production of protocorm-like bodies in orchid—Dendrobium ‘Sonia’. Sci Hortic 100:161–168

Sharma KKR, Sharma B, Majumdar S (2005) Micropropagation of Dendrobium fimbriatum Hook. by green pod culture. J Plant Biol 48:253–257

Singh A, Duggal S (2009) Medicinal orchids: an overview. Ethnobot Leaflets 13:351–363

Smith SE, Read DJ (2008) Mycorrhizal symbiosis, 3 edn. Academic Press/Elsevier, London

Soares JS, Rosa YBCJ, Suzuki RM, Scalon SPQ, Rosa Junior EJ (2012) Asymbiotic germination and development of Dendrobium nobile Lindl. under the effect of plant growth regulators in pre-germinative treatment. Rev Bras Plant Med 14:617–623 (in Portuguese with English abstract)

Soares JS, Rosa YBCJ, Suzuki RM, Scalon SPQ, Rosa Junior EJ (2013) Cultivo in vitro de Dendrobium nobile com uso de água de coco no meio de cultura. Hortic Bras 31:63–67 (in Portuguese with English abstract)

Soares JS, Rosa YBCJ, Sorgato JC, Rosa DBCJ, Pereira STS (2014) Utilização de agentes geleificantes alternativos no cultivo in vitro de Dendrobium nobile Lindl. Enciclopédia Biosfera 10:1832–1838 (in Portuguese with English abstract)

Song MKR, Silva GL, Faria RT, Takahashi LSA (1999) Análise do crescimento e enraizamento in vitro de híbridos de Dendrobium nobile Lindl. (Orchidaceae) semeados em diferentes meios de cultura. In: Congresso Brasileiro de Floricultura e Plantas Ornamentais, vol 12. Anais do Congresso Brasileiro de Floricultura e Plantas Ornamentais, Brasil, p 110 (abstract)

Song XQ, Guo N, Zhan XC, Nie L, Song QQ (2004) Research of non-symbiotic sproution of Dendrobium nobile Lindl and germplasm storage. J Guizhou Normal Univ (Nat Sci) 22:13–16 (in Chinese with English abstract)

Soundararajan T (2009) Micropropagation studies on Bacopa monnieri, Nerium oleander and Bioreactor studies on micropropagation of Dendrobium, an orchid. PhD thesis, Bharath University, India, p 191. http://shodhganga.inflibnet.ac.in/handle/10603/51 . Accessed 12 June 2015

Su MJ, Schnitzer JA, Faria RT (2012) Polpa de banana e fertilizantes comerciais no cultivo in vitro de orquídea. Científica 40:28–34 (in Portuguese with English abstract)

Sun YY, Li HA, Yan H, Li K (2009) Asepsis sowing and tissue culture of Dendrobium devonianum Paxt. Plant Physiol Commun 45:1017–1018 (in Chinese)

Sunitibala H, Kishor R (2009) Micropropagation of Dendrobium transparens L. from axenic pseudobulb segments. Indian J Biotech 8:448–452

Swangmaneecharern P, Serivichyaswat P, Nontachaiyapoom S (2012) Promoting effect of orchid mycorrhizal fungi Epulorhiza isolates on seed germination of Dendrobium orchids. Sci Hortic 148:55–58

Swarts ND, Dixon KW (2009) Terrestrial orchid conservation in the age of extinction. Ann Bot 104:543–556

Tang GX, Wang FD, Zhou WJ (2005) Studies on the seed embryo germination and propagation of Dendrobium candidum in vitro. China J Chinese Materia Medica 30:1583–1586 (in Chinese with English abstract)

Teixeira da Silva JA (2012) Is BA (6-benzyladenine) BAP (6-benzylaminopurine)? Asian Australas J Plant Sci Biotech 6:121–124

Teixeira da Silva JA (2013) Orchids: advances in tissue culture, genetics, phytochemistry and transgenic biotechnology. Floric Ornam Biotech 7:1–52

Teixeira da Silva JA, Aceto S, Liu W, Yu H, Kanno A (2014a) Genetic control of flower development, color and senescence of Dendrobium orchids. Sci Hortic 175:74–86

Teixeira da Silva JA, Zeng SJ, Dobránszki J, Cardoso JC, Kerbauy GB (2014b) In vitro flowering of Dendrobium. Plant Cell Tissue Organ Cult 119:447–456

Teixeira da Silva JA, Zeng SJ, Dobránszki J, Galdiano R Jr, Cardoso JC, Vendrame WA (2014c) Preservation of Dendrobium germplasm. Plant Cell Rep 33:1413–1423

Teixeira da Silva JA, Dobránszki J, Cardoso JC, Zeng SJ (2015a) Micropropagation of Dendrobium: a review. Plant Cell Rep 34:671–704

Teixeira da Silva JA, Tsavkelova E, Zeng SJ, Ng TB, Dobránszki J, Parthibhan S, Cardoso JC, Rao MV (2015b) Symbiotic in vitro seed propagation of Dendrobium: fungal and bacterial partners and their influence on plant growth and development. Planta 242:1–22

Thimijan RW, Heins RD (1983) Photometric, radiometric, and quantum light units of measure: a review of procedures for interconversion. HortScience 18:818–822

Thomale H (1954) Die Orchideen. Verlag Eugen Ulmer, Stuttgart

Udomdee W, Wen PJ, Lee CY, Chin SW, Chen FC (2014) Effect of sucrose concentration and seed maturity on in vitro germination of Dendrobium nobile hybrids. Plant Growth Regul 72:249–255

Vacin E, Went FW (1949) Some pH changes in nutrient solutions. Bot Gaz 110:605–613

Vasudevan R, Van Staden J (2010) Fruit harvesting time and corresponding morphological changes of seed integuments influence in vitro seed germination of Dendrobium nobile Lindl. Plant Growth Regul 60:237–246

Vendrame WA, Carvalho VS, Dias JMM, Maguire I (2008) Pollination of Dendrobium hybrids using cryopreserved pollen. HortScience 43:264–267

Vijayakumar S, Rajalkshmi G, Kalimuthu K (2012) Propagation of Dendrobium aggregatum through the culture of immature seeds from green capsules. Lankesteriana 12:131–135

Vyas S, Guha S, Bhattacharya M, Usha Rao I (2009) Rapid regeneration of plants of Dendrobium lituiflorum Lindl. (Orchidaceae) by using banana extract. Sci Hortic 121:32–37

Wang LH, Zhu YQ, Jiang YL, Feng YC (2007) Tissue culture and rapid propagation of Dendrobium thyrsiflorum Rchb. F. Plant Physiol Commun 43(5):894 (in Chinese)

Wolter KE (1968) Root and shoot initiation in aspen callus cultures. Nature 219:509–510

Wu HF, Song XQ, Liu HX (2012) Ex-situ symbiotic seed germination of Dendrobium catenatum. Acta Ecol Sin 32:2491–2497 (in Chinese with English abstract)

Xu YZ, Yu LW (1984) Sterile germination and in vitro propagation of Dendrobium tosaense. Plant Physiol Commun 4:35–36 (in Chinese with English abstract)

Yam TW, Arditti J (2009) History of orchid propagation: a mirror of the history of biotechnology. Plant Biotechnol Rep 3:1–56

Yang QG, Wang LA, Wang LZ (1989) Studies on in vitro culture of premature seeds of Dendrobium huoshanense Tang et Cheng. China J Chin Mater Med 14(3):19–20, 62 (in Chinese with English abstract)

Yang N, Chen ZL, Duan J, Zeng SJ (2006) In vitro propagation of Dendrobium cochliodes Schltr. Plant Physiol Commun 42(2):248 (in Chinese with English abstract)

Ye XL, Cheng SJ, Wang FX, Qian NF (1988) Morphology of immature seeds and development in in vitro of Dendrobium candidum. Acta Bot Yunnan 10:285–290 (in Chinese with English abstract)

Yu L, Lan QY, Tang GG (2011) Study on nonsymbiotic sproution of Dendrobium fimbriatum Hook. J Fujian Coll For 31:346–348 (in Chinese with English abstract)

Zeng SJ, Cheng SJ (1996) Tissue culture and rapid propagation of Dendrobium. J Chin Med Mater 19:490–491 (in Chinese with English abstract)

Zeng SJ, Cheng SJ, Zhang JL, Zhao FP (1998) Embryo culture and propagation of Dendrobium in vitro. Acta Horti Sin 25:75–80 (in Chinese with English abstract)

Zeng S, Wu K, Teixeira da Silva JA, Zhang J, Chen Z, Xia N, Duan J (2012) Asymbiotic seed germination, seedling development and reintroduction of Paphiopedilum wardii Sumerh., an endangered terrestrial orchid. Sci Hortic 138:198–209

Zeng SJ, Teixeira da Silva JA, Wu KL, Zhang JX, Duan J (2014) Seed biology and in vitro seed germination of Cypripedium. Crit Rev Biotechnol 34:358–371

Zeng SJ, Huang WC, Wu KL, Zhang JX, Teixeira da Silva JA, Duan J (2015) In vitro propagation of Paphiopedilum orchids. Crit Rev Biotechnol. doi: 10.3109/07388551.2014.993585

Zhan QC, Li X, Qi Y, Yang SX, Ye QM, Jian LG (2010) The rapid propagation of Dendrobium wilsonii Rolfe and its plantlet. North Hortic 16:137–139 (in Chinese with English abstract)

Zhao D, Hu G, Chen Z, Shi Y, Zheng L, Tang A, Long C (2013a) Micropropagation and in vitro flowering of Dendrobium wangliangii: a critically endangered medicinal orchid. J Med Plants Res 7:2098–2110

Zhao MM, Zhang G, Zhang DW, Hsiao YY, Guo SX (2013b) ESTs analysis reveals putative genes involved in symbiotic seed germination in Dendrobium officinale. PLoS One 8:e72705

Zhou Y, Yin LQ, Tang GJ, Huang WC, Hu YH, Li XF, Jiang JP, Liu Z, Hu HF (2012) Establishment of technological system for in vitro propagation of Dendrobium aduncum. J Shanghai Jiaotong Univ (Agric Sci) 30(6):62–66 (in Chinese with English abstract)