Association of polymorphisms in angiotensin II receptor genes with aldosterone-producing adenoma

Journal of Tongji Medical University - Tập 31 - Trang 301-305 - 2011
Jinzhi Ouyang1, Zhun Wu2, Jinchun Xing2, Yongji Yan3, Guoxi Zhang4, Baojun Wang5, Hongzhao Li5, Xin Ma5, Xu Zhang5
1Department of Endocrinology, Chinese PLA General Hospital, Beijing, China
2Department of Urology, The First Affiliated Hospital of Xiamen University, Xiamen, China
3Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China
4Department of Urology, The First Affiliated Hospital of Gannan Medical University, Ganzhou, China
5Department of Urology, Chinese PLA General Hospital, Beijing, China

Tóm tắt

This study examined the association of polymorphisms in angiotensin II receptor genes (AT 1 R and AT 2 R) with the risk for aldosterone-producing adenoma (APA) in a Chinese Han population. Four polymorphisms including rs5182 (573T/C) in exon 4, rs5186 (1166A/C) in 3′-untranslated region (3′-UTR) in AT 1 R gene and rs5194 (2274G/A) in 3′-UTR, rs1403543 (1675G/A) in intron 1 in AT 2 R gene were detected in 148 APA patients and 192 normal subjects (serving as control) by using a MGB-Taqman probe. The distribution of genotypes of each locus was in accordance with Hardy-Weinberg Equilibrium (HWE) in the APA and control groups (P>0.05). The allele A frequency at rs5194 was significantly higher in the APA group (0.49) than in the control group (0.35) (χ 2=12.08, P=0.001). Subjects with homozygotic genotype AA and heterozygotic genotype GA were at an increased risk for APA as compared to those with GG genotype (OR=2.66, 95% CI=1.45–4.87; OR=1.67, 95% CI=1.02–2.74). Furthermore, rs5194 single-nucleotide polymorphism (SNP) at AT 2 R gene was significantly associated with APA in additive (OR=1.64, 95% CI=1.21–2.20, P=0.001), dominant (OR=1.94, 95% CI=1.23–3.06, P=0.003), and recessive model (OR=2.01, 95% CI=1.17–3.45, P=0.01). It was concluded that rs5194 polymorphism at AT 2 R gene was associated with the risk for APA, which may constitute a genetic marker of APA.

Tài liệu tham khảo

Fallo F, Pistorello M, Pedini F, et al. In vitro evidence for local generation of renin and angiotensin II/III immunoreactivity by the human adrenal gland. Acta Endocrinol (Copenh), 1991,125(3):319–330 Gard PR. The role of angiotensin II in cognition and behavior. Eur J Pharmacol, 2002,438(1–2):1–14 Thomas WG, Mendelsohn FA. Angiotensin receptors: form and function and distribution. Int J Biochem Cell Biol, 2003,35(6):774–779 Alfakih K, Maqbool A, Sivananthan M, et al. Left ventricle mass index and the common, functional, X-linked angiotensin II type-2 receptor gene polymorphism (−1332 G/A) in patients with systemic hypertension. Hypertension, 2004,43(6):1189–1194 Schmieder RE, Erdmann J, Delles C, et al. Effect of the angiotensin II type 2-receptor gene (+1675 G/A) on left ventricular structure in humans. J Am Coll Cardiol, 2001,37(1):175–182 Koh WP, Yuan JM, Van Den Berg D, et al. Polymorphisms in angiotensin II type 1 receptor and angiotensin I-converting enzyme genes and breast cancer risk among Chinese women in Singapore. Carcinogenesis, 2005,26(2):459–464 de Gasparo M, Catt KJ, Inagami T, et al. International union of pharmacology. XXIII. The angiotensin II receptors. Pharmacol Rev, 2000,52(3):415–472 Martin MM, Elton TS. The sequence and genomic organization of the human type 2 angiotensin II receptor. Biochem Biophys Res Commun, 1995,209(2):554–562 Vinson GP, Ho MM, Puddefoot JR. The distribution of angiotensin II type 1 receptors, and the tissue renin-angiotensin systems. Mol Med Today, 1995,1(1): 35–39 Aguilera G. Factors controlling steroid biosynthesis in the zona glomerulosa of the adrenal. J Steroid Biochem Mol Biol, 1993,45(1–3):147–151 Hilbers U, Peters J, Bornstein SR, et al. Local renin-angiotensin system is involved in K+-induced aldosterone secretion from human adrenocortical NCI-H295 cells. Hypertension, 1999,33(4):1025–1030 Belloni AS, Andreis PG, Macchi V, et al. Distribution and functional significance of angiotensin-II AT1- and AT2-receptor subtypes in the rat adrenal gland. Endocr Res, 1998,24(1):1–15 Nakajima M, Hutchinson HG, Fujinaga M, et al. The angiotensin II type 2 (AT2) receptor antagonizes the growth effects of the AT1 receptor: gain-of-function study using gene transfer. Proc Natl Acad Sci U S A, 1995,92(23):10 663–10 667 Inagami T, Eguchi S, Numaguchi K, et al. Cross-talk between angiotensin II receptors and the tyrosine kinases and phosphatases. J Am Soc Nephrol, 1999,10(Suppl): 11S57–11S61 Widdop RE, Jones ES, Hannan RE, et al. Angiotensin AT2 receptors: cardiovascular hope or hype? Br J Pharmacol, 2003,140(5):809–824 Xu J, Carretero OA, Liu YH, et al. Role of AT2 receptors in the cardioprotective effect of AT1 antagonists in mice. Hypertension, 2002,40(3):244–250 Wu L, Iwai M, Nakagami H, et al. Effect of angiotensin II type 1 receptor blockade on cardiac remodeling in angiotensin II type 2 receptor null mice. Arterioscler Thromb Vasc Biol, 2002,22(1):49–54 Bonnardeaux A, Davies E, Jeunemaitre X, et al. Angiotensin II type 1 receptor gene polymorphisms in human essential hypertension. Hypertension, 1994,24(1): 63–69 Kainulainen K, Perola M, Terwilliger J, et al. Evidence for involvement of the type 1 angiotensin II receptor locus in essential hypertension. Hypertension, 1999,33(3): 844–849 Diez J, Laviades C, Orbe J, et al. The A1166C polymorphism of the AT1 receptor gene is associated with collagen type I synthesis and myocardial stiffness in hypertensives. J Hypertens, 2003,21(11):2085–2092 Zhang M, Ma H, Wang BS, et al. Angiotensin II type 2 receptor gene polymorphisms and cardioprotective role in essential hypertension. Heart Vessels, 2006,21(2):95–101 Wu Z, Ni D, Yan Y, et al. Expression of angiotensin IIreceptors in aldosterone-producing adenoma of the adrenal gland and their clinical significance. J Huazhong Univ Sci Technolog Med Sci, 2010,30(4):486–489 Jin XQ, Fukuda N, Su JZ, et al. Angiotensin II type 2 receptor gene transfer downregulates angiotensin II type 1a receptor in vascular smooth muscle cells. Hypertension, 2002,39(5):1021–1027 Kuznetsova T, Staessen JA, Thijs L, et al. Left ventricular mass in relation to genetic variation in angiotensin II receptors, renin system genes, and sodium excretion. Circulation, 2004,110(17):2644–2650 Herrmann SM, Nicaud V, Schmidt-Petersen K, et al. Angiotensin II type 2 receptor gene polymorphism and cardiovascular phenotypes: the GLAECO and GLAOLD studies. Eur J Heart Fail, 2002,4(6):707–712 Yamada T, Horiuchi M, Dzau VJ. Angiotensin II type 2 receptor mediates programmed cell death. Proc Natl Acad Sci USA, 1996,93(1):156–160 Stoll M, Unger T. Angiotensin and its AT2 receptor: new insights into an old system. Regul Pept, 2001,99(2–3): 175–182