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Đánh giá tác động đặc hiệu của cadmium lên hệ thống phòng thủ chống oxy hóa và peroxid hóa lipid trong cá chạch nước ngọt, Channa punctatus
Tóm tắt
Các tác động của nồng độ khác nhau của cadmium chloride lên mức độ peroxid hóa lipid (LPO) và sự thay đổi trong hoạt động của các enzyme chống oxy hóa đã được nghiên cứu trong các mô gan, thận và mang của cá chạch nước ngọt, Channa punctatus. Các mẫu cá đã được tiếp xúc với các nồng độ không gây chết 6.7, 13.4 và 20.1 mg l−1 cadmium chloride và tình trạng stress oxy hóa đã được đánh giá sau 24, 48, 72 và 96 giờ sau khi tiếp xúc. Các chỉ số sinh học được chọn cho nghiên cứu bao gồm các chất phản ứng với axit thiobarbituric nhằm đánh giá mức độ peroxid hóa lipid và hệ thống phòng thủ chống oxy hóa như glutathione dạng khử (GSH), glutathione reductase (GR), glutathione peroxidase (GPX), glutathione-S-transferase (GST), catalase (CAT) và hoạt động của superoxide dismutase (SOD). Nói chung, sự tiếp xúc với cadmium đã làm tăng mức độ LPO trong các mô của nhóm điều trị và điều chỉnh hoạt động của GPx, GST, SOD, CAT, GR và mức độ GSH sau khi tiếp xúc so với nhóm đối chứng. Tất cả các hoạt động enzyme, ngoại trừ CAT (trong thận và mang), và lượng LPO đã tăng đáng kể (P < 0.05) trong nhóm điều trị so với đối chứng ở tất cả các mô, trong khi sự khác biệt đáng kể không được quan sát giữa các nồng độ tiếp xúc và trong thời gian tiếp xúc. Kết quả cho thấy rằng sự tăng lên của mức độ LPO và sự dao động trong hệ thống phòng thủ chống oxy hóa ở cá có thể do sự gia tăng sản xuất các loài oxy phản ứng (ROS) gây ra bởi cadmium. Vai trò tiềm năng của các thông số này như là chỉ số sinh học của ô nhiễm kim loại nặng trong hệ sinh thái nước được thảo luận.
Từ khóa
#cadmium #peroxid hóa lipid #hệ thống phòng thủ chống oxy hóa #cá chạch nước ngọt #Channa punctatus #ô nhiễm kim loại nặngTài liệu tham khảo
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