Đánh giá tác động đặc hiệu của cadmium lên hệ thống phòng thủ chống oxy hóa và peroxid hóa lipid trong cá chạch nước ngọt, Channa punctatus

Fish Physiology and Biochemistry - Tập 38 - Trang 469-482 - 2011
Anurag Dabas1, N. S. Nagpure1, Ravindra Kumar1, B. Kushwaha1, Pavan Kumar1, W. S. Lakra2
1National Bureau of Fish Genetic Resources, Indian Council of Agricultural Research, Lucknow, India
2Central Institute of Fisheries Education, Mumbai, India

Tóm tắt

Các tác động của nồng độ khác nhau của cadmium chloride lên mức độ peroxid hóa lipid (LPO) và sự thay đổi trong hoạt động của các enzyme chống oxy hóa đã được nghiên cứu trong các mô gan, thận và mang của cá chạch nước ngọt, Channa punctatus. Các mẫu cá đã được tiếp xúc với các nồng độ không gây chết 6.7, 13.4 và 20.1 mg l−1 cadmium chloride và tình trạng stress oxy hóa đã được đánh giá sau 24, 48, 72 và 96 giờ sau khi tiếp xúc. Các chỉ số sinh học được chọn cho nghiên cứu bao gồm các chất phản ứng với axit thiobarbituric nhằm đánh giá mức độ peroxid hóa lipid và hệ thống phòng thủ chống oxy hóa như glutathione dạng khử (GSH), glutathione reductase (GR), glutathione peroxidase (GPX), glutathione-S-transferase (GST), catalase (CAT) và hoạt động của superoxide dismutase (SOD). Nói chung, sự tiếp xúc với cadmium đã làm tăng mức độ LPO trong các mô của nhóm điều trị và điều chỉnh hoạt động của GPx, GST, SOD, CAT, GR và mức độ GSH sau khi tiếp xúc so với nhóm đối chứng. Tất cả các hoạt động enzyme, ngoại trừ CAT (trong thận và mang), và lượng LPO đã tăng đáng kể (P < 0.05) trong nhóm điều trị so với đối chứng ở tất cả các mô, trong khi sự khác biệt đáng kể không được quan sát giữa các nồng độ tiếp xúc và trong thời gian tiếp xúc. Kết quả cho thấy rằng sự tăng lên của mức độ LPO và sự dao động trong hệ thống phòng thủ chống oxy hóa ở cá có thể do sự gia tăng sản xuất các loài oxy phản ứng (ROS) gây ra bởi cadmium. Vai trò tiềm năng của các thông số này như là chỉ số sinh học của ô nhiễm kim loại nặng trong hệ sinh thái nước được thảo luận.

Từ khóa

#cadmium #peroxid hóa lipid #hệ thống phòng thủ chống oxy hóa #cá chạch nước ngọt #Channa punctatus #ô nhiễm kim loại nặng

Tài liệu tham khảo

Aebi H (1984) Catalase in vitro In: Packer L (ed) Methods in enzymology, vol 105. Academic Press, Orlando, pp 121–126 Agrahari S, Pandey KC, Gopal K (2007) Biochemical alteration induced by monocrotophos in the blood plasma of fish Channa punctatus (Bloch). Pest Biochem Physiol 88:268–272 Ahmad I, Hamid T, Fatima M, Chand HS, Jain SK, Athar M, Raisuddin S (2000) Induction of hepatic antioxidants in freshwater catfish (Channa punctatus Bloch) is a biomarker of paper mill effluent exposure. Biochim Biophys Acta 1523:37–48 Ali M, Parvez S, Pandey S, Atif F, Kaur M, Rehman H, Raisuddin S (2004) Fly ash leachate induces oxidative stress in freshwater fish Channa punctata (Bloch). Environ Int 30:933–938 Ali D, Nagpure NS, Kumar S, Kumar R, Kushwaha B, Lakra WS (2009) Assessment of genotoxic and mutagenic effects of chlorpyrifos in freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis. Food Chem Toxicol 47:650–656 Almeida EA, Miyamoto S, Bainy ACD, Medeiros MHG, Di Mascio P (2004) Protective effects of phospholipid hydroperoxide glutathione peroxidase (PHGPx) against lipid peroxidation in mussels Perna perna exposed to different metals. Mar Pollut Bull 49:386–392 Almroth BC, Sturve J, Berglund A, Forlin L (2005) Oxidative damage in eelpout (Zoarces viviparous), measured as protein carbonyls and TBARS, as biomarkers. Aquat Toxicol 73:171–180 AMAP (1998) Assessment report: Arctic pollution issues Arctic monitoring and assessment programme. Oslo APHA, AWWA, WPCF (2005) Standard methods for the examination of water and wastewater, 21st edn. American Publication of Health Association, Washington, DC Asagba SO, Eriyamremu GE, Igberaese ME (2008) Bioaccumulation of cadmium and its biochemical effect on selected tissues of the catfish (Clarias gariepinus). Fish Physiol Biochem 34:61–69 Atif F, Parvez S, Pandey S, Ali M, Kaur M, Rehman H, Khan HA, Raisuddin S (2005) Modulatory effect of cadmium exposure on deltamethrin-induced oxidative stress in Channa punctata Bloch. Arch Environ Contam Toxicol 49:371–377 Atli G, Alptekin O, Tukel S, Canli M (2006) Response of catalase activity to Ag+, Cd2+, Cr6+, Cu2+ and Zn2+ in five tissues of freshwater fish Oreochromis niloticus. Compar Biochem Physiol Part C 143:218–224 Battaglini P, Andreozzi G, Antonucci R, Arcamone N, De Girolamo P, Ferrara L, Gargiulo G (1993) The effects of cadmium on the gills of the goldfish Carassius auratus (L) metal uptake and histochemical changes. Comp Biochem Physiol 104C:239–247 Bechard KM, Gillis PL, Wood CM (2008) Trophic transfer of Cd from larval chironomids (Chironomus riparius) exposed via sediment or waterborne routes, to zebrafish (Danio rerio) tissue-specific and subcellular comparisons. Aquat Toxicol 90:310–321 Bennett RO, Dooley JK (1982) Copper uptake by two sympatric species of killifish Fundulus heteroclitus (L) and L. majalis (Walkbaum). J Fish Biol 21:381–398 Berg JM, Tymoczko JL, Styer L (2002) Biochemistry, 5th edn. WH Freeman and Company, New York Blaha L, Kopp R, Simkova K, Mares J (2004) Oxidative stress biomarkers are modulated in silver carp (Hypophthalmichthys molitrix Val) exposed to microcystin-producing cyanobacterial water bloom. Acta Veterin BRNO 73:477–482 Bouraoui Z, Banni M, Ghedira J, Clerandeau C, Guerbej H, Narbonne JF, Boussetta H (2008) Acute effects of cadmium on liver phase I and phase II enzymes and metallothionein accumulation on sea bream Sparus aurata. Fish Physiol Biochem 34:201–207 Canesi L, Viarengo A, Leonzio C, Filippelli M, Gallo G (1999) Heavy metals and glutathione metabolism in mussel tissues. Aquat Toxicol 46:67–76 Carlberg I, Mannervik B (1975) Purification and characterization of the flavoenzyme glutathione reductase from rat liver. J Biol Chem 250:5475–5480 Cattani O, Serra R, Isani G, Raggi G, Cortesi P, Carpene E (1996) Correlation between metallothionein and energy metabolism in sea bass, Dicentrachus labrax, exposed to cadmium. Comp Biochem Physiol 113C:193–199 Cho MK, Kim SG (2000) Induction of class alpha glutathione S-transferases by 4-methylthiazole in the rat liver: role of oxidative stress. Toxicol Lett 115:107–115 Deviller G, Palluel O, Aliaume C, Asanthi H, Sanchez W, Franco Nava MA, Blancheton JP, Casellas C (2005) Impact assessment of various rearing systems on fish health using multibiomarker response and metal accumulation. Ecotoxicol Environ Saf 61:89–97 Draper HH, Squires EJ, Mahmooch H, Wu J, Agarwal S, Handley M (1993) A comparative evaluation of thiobarbituric acid methods for the determination of malondialdehyde in biological materials. Free Radic Biol Med 15:353–363 Elif OO, Üner N (2000) Combined effects of 2,4-D and azinphosmethyl on antioxidant enzymes and lipid peroxidation in liver of Oreochromis niloticus. Com Biochem Physiol Part C 127:291–296 Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70–77 Ercal N, Gurer-Orhan H, Aykin-Burns N (2001) Toxic metals and oxidative stress Part I: mechanisms involved in metal induced oxidative damage. Curr Topics Med Chem 1:529–539 Fernandes C, Fontaı′nhas-Fernandes A, Monteiro S, Salgado MA (2007) Changes in plasma electrolytes and gill histopathology in wild Liza saliens from the Esmoriz-Paramos coastal lagoon. Portugal Bull Environ Contam Toxicol 79:301–305 Ferreira M, Moradas-Ferreira P, Reis-Henriques MA (2005) Oxidative stress biomarkers in two resident species, mullet (Mugil cephalus) and flounder (Platichthys flesus), from a polluted site in River Douro Estuary. Portugal Aquat Toxicol 71:39–48 Ferreira M, Moradas-Ferreira P, Reis-Henriques MA (2006) The effect of long-term depuration on phase I and phase II biotransformation in mullets (Mugil cephalus) chronically exposed to pollutants in River Douro Estuary. Portugal Mar Environ Res 61:326–338 Finney DJ (1971) Probit analysis. University Press, Cambridge, p 333 Flohe L, Gunzler WA (1984) Assays of glutathione peroxidase. Methods Enzymol 21:105–114 Flohe L, Wingender E, Brigelius-Flohe R (1997) The regulation of glutathione peroxidases. In: Forman H, Cadenas E (eds) Oxidative stress and signal transduction. Chapman & Hall, New York, pp 415–435 Goering PL, Waalkes MP, Klaassen CD (1995) Toxicology of cadmium. In: Goyer RA, Cherian MG (eds) Toxicology of metals biochemical aspects. Springer, Berlin, pp 189–214 Habig W, Pabst M, Jakoby W (1974) Glutathione S-transferases: the first enzymatic step in mercapturic acid formation. J Biol Chem 22:7130–7139 Halliwell B, Gutteridge HMC (1999) Free radicals in biology and medicine. Oxford University Press, Oxford, pp 1–936 Hasspieler BM, Behar JV, Di Giulio RT (1994) Glutathione-dependent defense in channel catfish (Ictalurus punctatus) and brown bullhead (Ameriurus nebulosus). Ecotoxicol Environ Saf 28:82–90 Hermes-Lima M (2004) Oxygen in biology and biochemistry: role of free radicals. In: Storey KB (ed) Functional metabolism regulation and adaptation. Wiley-Liss, Hoboken, pp 319–368 Janero DR (1990) Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic Biol Med 9:515–540 Kakkar P, Das B, Viswanathan PN (1984) A modified spectroscopic assay of superoxide dismutase. Indian J Biochem Biophys 21:130–132 Kelly SA, Havrilla CM, Brady TC, Abramo KH, Levin ED (1998) Oxidative stress in toxicology: established mammalian and emerging piscine model systems. Environ Health Perspect 106:375–384 Kohen R, Nyska A (2002) Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicol Pathol 30:620–650 Kumar A, Rai DK, Sharma B, Pandey RS (2009) λ-cyhalothrin and cypermethrin induced in vivo alterations in the activity of acetylcholinesterase in a freshwater fish, Channa punctatus (Bloch). Pesticide Biochem Physiol 93:96–99 Lamb JG, Franklin MR (2000) Early events in the induction of rat hepatic UDP-glucuronosyl-transferase, glutathione S-transferase and microsomal epoxide hydrolase by 1,7-phenanthroline: comparison with oltipraz, tert-butyl-4-hydroxyanisole, and tert butylhydroquinone. Drug Metab Dispos 28:1018–1026 Lemaire-Gony S, Lemaire P (1992) Interactive effects of cadmium and benzo(a)pyrene on cellular structure and biotransformation enzymes of the liver of the European eel Anguilla anguilla. Aquat Toxicol 22:145–160 Lesser MP (2006) Oxidative stress in marine environments: biochemistry and physiological ecology. Ann Rev Physiol 68:253–278 Li XY, Chung IK, Kim JI, Lee JA (2005) Oral exposure to Microcystis increases activity-augmented antioxidant enzymes in the liver of loach (Misgurnus mizolepis) and has no effect on lipid peroxidation. Comp Biochem Physiol 141:292–296 Livingstone DR (2001) Contaminant reactive oxygen species production and oxidative damage in aquatic organisms. Mar Pollut Bull 42:656–666 Livingstone DR (2003) Oxidative stress in aquatic organisms in relation to pollution and aquaculture. Rev Med Vet 154:427–430 Lowry OH, Rosenbrough NM, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275 Lushchak V, Lushchak LP, Mota AA, Hermes-Lima M (2001) Oxidative stress and antioxidant defences in goldfish Carassius auratus during anoxia and reoxygenation. Am J Physiol Regul Integr Comp Physiol 280:100–107 Michiels C, Raes M, Toussaint O, Remacle J (1994) Importance of Se-glutathione peroxidase, catalase, and Cu/Zn-SOD for cell survival against oxidative stress. Free Radic Biol Med 17:235–248 Miyamoto S, Dupas C, Murota K, Terao J (2003) Phospholipid hydroperoxides are detoxified by phospholipase A2 and GSH peroxidase in rat gastric mucosa. Lipids 38:641–649 Nogueira CW, Quinhones EB, Jung EAC, Zeni G, Rocha JBT (2003) Anti-Inflammatory and antinociceptive activity of biphenyl diselenide. Inflamm Res 52:56–63 Nwani CD, Lakra WS, Nagpure NS, Kumar R, Kushwaha B, Srivastava SK (2010) Toxicity of the herbicide atrazine: effects on lipid peroxidation and activities of antioxidant enzymes in the freshwater fish Channa Punctatus (Bloch). Int J Environ Res Public Health 7:3298–3312 Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351 Olsson PE, Larsson A, Haux C (1996) Influence of seasonal changes in water temperature on cadmium inducibility of hepatic and renal metallothionein in rainbow trout. Mar Environ Res 42:41–44 Organization for Economic Cooperation and Development (OECD) (1992) Guideline for the testing of chemicals: fish, acute toxicity test, Document 203 Oruc EO, Sevgiler Y, Uner N (2004) Tissue-specific oxidative stress responses in fish exposed to 2,4-D and azinphosmethyl. Comp Biochem Physiol 137:43–51 Osman HAM, Ibrahim TB, Ali AT, Derwa HIM (2009) Field application of humic acid against the effect of cadmium pollution on cultured Tilapia Oreochromis niloticus. World Appl Sci J 6:1569–1575 OSPAR (2002) Cadmium hazardous substances series OSPAR commission (2002) Ozmen I, Bayir A, Cengiz M, Sirkecioglu AN, Atamanalp M (2004) Effects of water reuse system on antioxidant enzymes of rainbow trout (Oncorhynchus mykiss W, 1792). Veterin Med Czech 49:373–378 Pandey S, Ahmad I, Parvez S, Bin-Hafeez B, Haque R, Raisuddin S (2001) Effect of endosulfan on antioxidants of freshwater fish Channa punctatus Bloch: 1. Protection against lipid peroxidation in liver by copper preexposure. Arch Environ Contam Toxicol 41:345–352 Pandey S, Nagpure NS, Kumar R, Sharma S, Srivastava SK, Verma MS (2006) Genotoxicity evaluation of acute doses of endosulfan to freshwater teleost Channa punctatus (Bloch) by alkaline single-cell gel electrophoresis. Ecotoxicol Environ Saf 65:56–61 Pandey S, Parvez S, Ansari RA, Ali M, Kaur M, Hayat F, Ahmad F, Raisuddin S (2008) Effects of exposure to multiple trace metals on biochemical, histological and ultrastructural features of gills of a freshwater fish, Channa punctata Bloch. Chemico-Biol Interact 174:183–192 Paris-Palacios S, Biagianti-Risbourg S, Vernet G (2000) Biochemical and (ultra)structural hepatic perturbations of Brachydanio rerio (Teleostei, Cyprinidae) exposed to two concentrations of copper sulphate. Aquat Toxicol 50:109–124 Regoli F, Frenzilli G, Bochetti R, Annarumma F, Scarcelli V, Fattorini D, Nigro N (2004) Time-course variations of oxyradical metabolism, DNA integrity and lysosomal stability in mussels, Mytilus galloprovincialis, during a filed translocation experiment. Aquat Toxicol 68:167–178 Risso-de Faverney C, Orsini N, de Sousa G, Rahmani R (2004) Cadmium induced apoptosis through the mitochondrial pathway in rainbow trout hepatocytes: involvement of oxidative stress. Aquat Toxicol 69:247–258 Ritola O, Livingstone DR, Peters LD, Lindstrom-Seppa P (2002) Antioxidant processes are affected in juvenile rainbow trout (Oncorhynchus mykiss) exposed to ozone and oxygen-supersaturated water. Aquaculture 210:1–19 Roche H, Boge G (1996) Fish blood parameters as a potential tool for identification of stress caused by environmental factors and chemical intoxication. Mar Environ Res 41:27–43 Romeo M, Bennani N, Gnassia-Barelli M, La Faurie M, Girard JP (2000) Cadmium and copper display different responses towards oxidative stress in the kidney of the sea bass Dicentrarchus labrax. Aquat Toxicol 48:185–194 Sayeed I, Parvez S, Pandey S, Bin-Hafeez B, Haque R, Raisuddin S (2003) Oxidative stress biomarkers of exposure to deltamethrin in freshwater fish, Channa punctatus Bloch. Ecotoxicol Environ Saf 56:295–301 Schlenk D, Rice CD (1998) Effects of zinc and cadmium treatment on hydrogen peroxide-induced mortality and expression of glutathione and metallothionein in a teleost hepatoma cell line. Aquat Toxicol 43:121–129 Sharma S, Nagpure NS, Kumar R, Pandey S, Singh PJ, Srivastava SK, Mathur PK (2007) Studies on the genotoxicity of endosulfan in different tissue of fresh water fish Mystus vittatus using the Comet assay. Arch Environ Contam Toxicol 53:617–623 Sies H (1993) Strategies of antioxidant defenses. Eur J Biochem 215:213–219 Soares SS, Aureliano M, Joaquim N, Coucelo JM (2003) Cadmium and vanadate oligomers effects on methaemoglobin reductase activity from Lusitanian toadfish: in vivo and in vitro studies. J Inorg Biochem 94:285–290 Soares SS, Martins H, Gutiérrez-Merino C, Aureliano M (2008) Vanadium and cadmium in vivo effects in teleost cardiac muscle: metal accumulation and oxidative stress markers. Compar Biochem Physiol Part C 147:168–178 Stohs SJ, Hagchi D, Hassoun E, Bagshi M (2000) Oxidative mechanisms in the toxicity of chromium and cadmium ions. J Environ Pathol Toxicol Oncol 19:201–213 Thomas P, Wofford W (1984) Effects of metals and organic compounds on hepatic glutathione, cysteine and acid soluble thiol-levels in mullet (Mugil cephaius). Toxicol Appl Pharmacol 76:172–182 Tort L, Krgacin B, Torres P, Giralt M, Hidalgo J (1996) The effect of cadmium exposure and stress on plasma cortisol, metallothionein levels and oxidative status in rainbow trout (Oncorhynchus mykiss) liver. Comp Biochem Physiol C-Pharm Toxicol Endocr 144:29–34 Vaglio A, Landriscina C (1999) Changes in liver enzyme activity in the teleost Sparus aurata in response to cadmium intoxication. Ecotoxicol Environ Safe 43:111–116 Wright DA, Welbourn PM (1994) Cadmium in the aquatic environment: a review of ecological, physiological, and toxicological effects on biota. Environ Rev 2:187–214