Assessment of genetic diversity of Saltol QTL among the rice (Oryza sativa L.) genotypes

Physiology and Molecular Biology of Plants - Tập 22 - Trang 107-114 - 2016
Showkat Ahmad Ganie1, Mrinmoi Jyoti Borgohain1, Kashyap Kritika1, Akshay Talukdar2, Dipti Ranjan Pani3, Tapan Kumar Mondal1
1Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, Pusa, New Delhi, India
2Division of Genetics, ICAR-Indian Agricultural Research Institute, Pusa, New Delhi, India
3NBPGR Base Centre, ICAR-NRRI Campus, Cuttack, India

Tóm tắt

Eight Saltol quantitative trait locus (QTL) linked simple sequence repeat (SSR) markers of rice (Oryza sativa L.) were used to study the polymorphism of this QTL in 142 diverse rice genotypes that comprised salt tolerant as well as sensitive genotypes. The SSR profiles of the eight markers generated 99 alleles including 20rare alleles and 16 null alleles. RM8094 showed the highest number (13) of alleles followed by RM3412 (12), RM562 (11), RM493 (9) and RM1287 (8) while as, RM10764 and RM10745 showed the lowest number (6) of alleles. Based on the highest number of alleles and PIC value (0.991), we identified RM8094 as suitable marker for discerning salt tolerant genotypes from the sensitive ones. Based upon the haplotype analysis using FL478 as a reference (salt tolerant genotypes containing Saltol QTL), we short listed 68 rice genotypes that may have at least one allele of FL478 haplotype. Further study may confirm that some of these genotypes might have Saltol QTL and can be used as alternative donors in salt tolerant rice breeding programmes.

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