Sự giảm nhẹ độc tính do mangan gây ra trong Vallisneria natans (Lour.) Hara nhờ axit ascorbic

Pei Fan1, Jun Yin1,2, Guidi Zhong1, Zhonghua Wu1
1Water Pollution Ecology Laboratory, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of China
2Shanghai Water Engineering Design & Research Institute Co., Ltd, Shanghai, People’s Republic of China

Tóm tắt

Chúng tôi đã khảo sát sự phát triển và phản ứng sinh lý của loài thực vật nước ngập Vallisneria natans (Lour.) Hara đối với 80 mg L−1 mangan (Mn) với các liều axit ascorbic (AsA 0, 5, 10, 20, 50, 100, 200 mg L−1) sau 21 ngày điều trị. Căng thẳng Mn đã làm giảm đáng kể số lượng lá cuối cùng và hoạt động của superoxide dismutase (SOD), catalase (CAT) và ascorbate peroxidase (APX) của V. natans, trong khi làm tăng khả năng chứa malondialdehyde (MDA), hydrogen peroxide (H2O2) và proline, cũng như hoạt động của peroxidase (POD), mà không có sự khác biệt có ý nghĩa trong tỷ lệ tăng trưởng tương đối (RGR) và hàm lượng sắc tố quang hợp của cây. Khi liều bổ sung AsA tăng lên (≤ 50 mg L−1), hàm lượng MDA giảm dần, trong khi hàm lượng proline, protein hòa tan, và sắc tố quang hợp, cũng như hoạt động của các enzyme chống oxy hóa (trừ POD) và RGR của V. natans tăng lên. Mức AsA ≥ 100 mg L−1 đã làm trầm trọng thêm độc tính của Mn trong V. natans bằng cách giảm đáng kể hoạt động của các enzyme chống oxy hóa và hàm lượng sắc tố quang hợp, thậm chí gây ra tác động gây chết cây. Những kết quả này gợi ý rằng căng thẳng Mn được nghiên cứu trong bài báo này có thể gây ra stress oxy hóa và ảnh hưởng đến sự phát triển của V. natans. Hơn nữa, liều AsA tối ưu có thể làm giảm stress oxy hóa do Mn gây ra là từ 41,37–50,25 mg L−1 theo phân tích hồi quy dựa trên sự phát triển cây và phản ứng enzym.

Từ khóa

#Vallisneria natans #mangan #axit ascorbic #stress oxy hóa #enzyme chống oxy hóa

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