Cấu trúc cộng đồng vi sinh vật và vi khuẩn trong các hầm bioga hộ gia đình sử dụng nguyên liệu thô khác nhau tại Cao nguyên Thanh Hải

Biotechnology Letters - Tập 43 - Trang 1337-1348 - 2021
Rui Han1, Li Liu1, Yan Meng1, Hairong Han1, Rongbo Xiong1, Yi Li1, Laisheng Chen1
1Qinghai Key Laboratory of Vegetable Genetics and Physiology, Academy of Agriculture and Forestry, Qinghai University, Xining, China

Tóm tắt

Nghiên cứu hiện tại nhằm điều tra cấu trúc cộng đồng vi sinh vật trong các hầm bioga hộ gia đình sử dụng nguyên liệu thô khác nhau ở vùng nông thôn Cao nguyên Thanh Hải. Phân tích giải trình tự gen 16S rRNA công nghệ cao cho thấy các ngành vi khuẩn Firmicutes, Bacteroidetes và Proteobacteria là những phyla chiếm ưu thế nhất (64,08%). Nhóm Prevotella 7 là chi chiếm ưu thế nhất trong các hầm YL9 và YL10 (69,72% và 26,96%, tương ứng) sử dụng nguyên liệu thô từ rác thải thực vật. Trichococcus thể hiện độ phong phú cao nhất (14,55%) trong hầm YL1 sử dụng phân cừu và phân lợn. Clostridium sensu stricto 1 (13,89%) và Synergistaceae_uncultured (15,52%) là những chi có độ phong phú cao nhất trong hầm YL5 với nguyên liệu thô hỗn hợp (cụ thể là phân sữa, phân cừu và phân người). Thêm vào đó, Proteiniphilum và Pseudomonas thể hiện độ phong phú cao nhất trong số các chi vi khuẩn trong hầm YL4 sử dụng phân lợn. Methanomicrobiales là cộng đồng vi sinh vật cổ chiếm ưu thế nhất, dao động từ 13,35% đến 81,34% về độ phong phú. Methanocorpusculum thể hiện độ phong phú cao trong tất cả các hầm sử dụng nguyên liệu thô khác nhau. Methanogenium là chi vi sinh vật cổ phong phú nhất trong các hầm YL4 và YL6, nơi sử dụng phân lợn làm nguyên liệu thô chủ yếu. Hơn nữa, Methanosarcina và Methanosaeta thể hiện độ phong phú cao nhất trong hầm YL1 (55,03%) và YL9 (51,40%), tương ứng. Hơn nữa, nhiệt độ lên men và pH đều góp phần vào cấu trúc cộng đồng vi sinh vật cổ và vi khuẩn trong tất cả các hầm được điều tra. Đặc biệt, nhiệt độ lên men cho thấy mối tương quan tích cực với độ phong phú của Synergistaceae_uncultured, Methanogenium và Methanosaeta, trong khi pH có mối tương quan tích cực với độ phong phú của nhóm Prevotella 7 và Methanosarcina.

Từ khóa

#vi sinh vật #hầm bioga #nguyên liệu thô #phân tích gene 16S rRNA #Cao nguyên Thanh Hải

Tài liệu tham khảo

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