Arbuscular mycorrhizal symbiosis is accompanied by local and systemic alterations in gene expression and an increase in disease resistance in the shoots

Plant Journal - Tập 50 Số 3 - Trang 529-544 - 2007
Jinyuan Liu1, Ignacio E. Maldonado‐Mendoza1, Melina López‐Meyer1, Foo Cheung2, Christopher D. Town2, Maria Harrison1
1Boyce Thompson Institute for Plant Research, Cornell University, Tower Road, Ithaca, NY14853, USA, and
2The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA

Tóm tắt

SummaryIn natural ecosystems, the roots of many plants exist in association with arbuscular mycorrhizal (AM) fungi, and the resulting symbiosis has profound effects on the plant. The most frequently documented response is an increase in phosphorus nutrition; however, other effects have been noted, including increased resistance to abiotic and biotic stresses. Here we used a 16 000‐feature oligonucleotide array and real‐time quantitative RT‐PCR to explore transcriptional changes triggered in Medicago truncatula roots and shoots as a result of AM symbiosis. By controlling the experimental conditions, phosphorus‐related effects were minimized, and both local and systemic transcriptional responses to the AM fungus were revealed. The transcriptional response of the roots and shoots differed in both the magnitude of gene induction and the predicted functional categories of the mycorrhiza‐regulated genes. In the roots, genes regulated in response to three different AM fungi were identified, and, through split‐root experiments, an additional layer of regulation, in the colonized or non‐colonized sections of the mycorrhizal root system, was uncovered. Transcript profiles of the shoots of mycorrhizal plants indicated the systemic induction of many genes predicted to be involved in stress or defense responses, and suggested that mycorrhizal plants might display enhanced disease resistance. Experimental evidence supports this prediction, and mycorrhizal M. truncatula plants showed increased resistance to a virulent bacterial pathogen, Xanthomonas campestris. Thus, the symbiosis is accompanied by a complex pattern of local and systemic changes in gene expression, including the induction of a functional defense response.

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Tài liệu tham khảo

10.1111/j.1439-0434.2005.01018.x

10.1111/j.1469-8137.1988.tb00273.x

10.1016/0076-6879(66)08014-5

10.1126/science.1092986

10.1111/j.1469-8137.1988.tb03698.x

10.1007/s10658-004-4585-7

Bonfante‐Fasolo P., 1984, VA Mycorrhizae, 5

10.1146/annurev.phyto.41.052002.095505

10.1111/j.1469-8137.1991.tb01013.x

10.1007/s00572-003-0263-4

Burleigh S.H., 1998, Phosphorous in Plant Biology: Regulatory Roles in Molecular Cellular, Organismic, and Ecosystem Processes, 359

10.1046/j.1365-313X.2003.01867.x

10.1094/MPMI.1998.11.10.1017

10.1016/j.fgb.2004.08.005

10.1146/annurev.phyto.42.040803.140421

10.1105/tpc.12.6.963

10.1038/nature00842

10.1094/MPMI-18-0771

10.1094/MPMI.1998.11.5.393

Gianinazzi‐Pearson V., 1996, Plant cell responses to arbuscular myorrhiza fungi: getting to the roots of the symbiosis, Plant Cell, 8, 1871, 10.2307/3870236

10.1007/s004250000323

10.1146/annurev.phyto.43.040204.135923

10.1111/j.1469-8137.1982.tb03347.x

10.1073/pnas.0502999102

10.1007/s00425-003-1136-y

10.1007/s11103-005-5567-7

10.1146/annurev.micro.58.030603.123749

10.1094/MPMI-6-643

10.1046/j.1365-313X.1994.6010009.x

10.1105/tpc.004861

10.1104/pp.104.056572

10.1071/FP06079

10.1038/nature03237

10.1105/tpc.105.035394

10.1093/nar/gkf685

10.1073/pnas.0508883103

10.1016/j.jbiotec.2003.11.011

10.1126/science.1093038

10.1093/pcp/pci231

10.1105/tpc.014183

10.1139/b04-048

10.1016/j.gene.2004.10.028

10.1094/MPMI.2004.17.10.1063

10.1111/j.1469-8137.1990.tb00476.x

10.1111/j.1469-8137.1978.tb01589.x

10.1111/j.1365-313X.2006.02788.x

10.1073/pnas.0400595101

10.1016/j.pbi.2004.05.011

10.1016/j.pbi.2006.05.008

10.1093/nar/29.9.e45

10.1105/tpc.8.8.1225

10.1093/jexbot/53.368.525

10.1104/pp.105.067603

Sanders F.E., 1974, Endomycorrhizas

10.1007/s11103-005-8102-y

Schwab S.M., 1983, Comparison of stages of vesicular–arbuscular mycorrhiza formation in sudangrass grown at two levels of phosphorus nutrition, Am. J. Bot., 70, 1225

10.1111/j.1469-8137.1991.tb00002.x

10.1006/mpev.2001.1007

Searle I.R., 2002, Long‐distance signaling in nodulation directed by a CLAVATA1‐like receptor kinase, Science, 299, 1

10.1094/MPMI.1999.12.11.1000

Smith S.E., 1997, Mycorrhizal Symbiosis

10.1071/PP9890033

10.1111/j.1469-8137.2004.01039.x

10.1104/pp.104.040873

10.1016/S0953-7562(96)80164-X

10.1038/nature00841

Terras F.R.G., 1995, Small cysteine‐rich antifungal proteins from radish – their role in host‐defense, Plant Cell, 7, 573

10.1111/j.1469-8137.1991.tb00028.x

10.1094/MPMI.1999.12.11.951

10.1078/0176-1617-01097

10.1016/S0038-0717(99)00155-8

10.1017/S0953756204231173

10.1017/S0953756204000346

10.1094/MPMI.2004.17.12.1385

10.1094/MPMI.2003.16.4.306

10.1093/nar/30.4.e15

10.1093/pcp/pcg122

10.1038/sj.cr.7310053

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Plant Journal

Tập 50 Số 3

529-544

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