Arabidopsis HIPP27 is a host susceptibility gene for the beet cyst nematode Heterodera schachtii

Molecular Plant Pathology - Tập 19 Số 8 - Trang 1917-1928 - 2018
Zoran S. Radaković1, Muhammad Shahzad Anjam1, Elizabeth Escobar1, Divykriti Chopra1, Javier Cabrera2, Ana Cláudia Silva2, Carolina Escobar2, Mirosław Sobczak3, Florian M. W. Grundler1, Shahid Siddique1
1INRES–Molecular Phytomedicine Rheinische‐Friedrich‐Wilhelms‐University of Bonn D‐53115 Bonn Germany
2Facultad de Ciencias Ambientales y Bioquímica Universidad de Castilla‐La Mancha, Área de Fisiología Vegetal Avda, Carlos III, s/n, 45071 Toledo Spain
3Department of Botany, Warsaw University of Life Sciences, PL-02787 Warsaw Poland

Tóm tắt

SummarySedentary plant‐parasitic cyst nematodes are obligate biotrophs that infect the roots of their host plant. Their parasitism is based on the modification of root cells to form a hypermetabolic syncytium from which the nematodes draw their nutrients. The aim of this study was to identify nematode susceptibility genes in Arabidopsis thaliana and to characterize their roles in supporting the parasitism of Heterodera schachtii. By selecting genes that were most strongly upregulated in response to cyst nematode infection, we identified HIPP27 (HEAVY METAL‐ASSOCIATED ISOPRENYLATED PLANT PROTEIN 27) as a host susceptibility factor required for beet cyst nematode infection and development. Detailed expression analysis revealed that HIPP27 is a cytoplasmic protein and that HIPP27 is strongly expressed in leaves, young roots and nematode‐induced syncytia. Loss‐of‐function Arabidopsis hipp27 mutants exhibited severely reduced susceptibility to H. schachtii and abnormal starch accumulation in syncytial and peridermal plastids. Our results suggest that HIPP27 is a susceptibility gene in Arabidopsis whose loss of function reduces plant susceptibility to cyst nematode infection without increasing the susceptibility to other pathogens or negatively affecting the plant phenotype.

Từ khóa


Tài liệu tham khảo

10.1111/febs.12159

10.1007/s11103-008-9419-0

10.1007/978-1-59745-289-2_2

10.1111/mpp.12114

10.1046/j.1365-313x.1998.00343.x

10.1104/pp.103.027979

10.1038/nature12817

10.1016/j.plaphy.2013.05.016

10.1016/0014-4827(68)90403-5

10.1016/bs.abr.2014.12.009

10.1007/BF01273172

10.1038/s41598-017-07269-7

10.1105/tpc.114.135327

10.1163/156854107781352034

10.1104/pp.107.107367

10.1111/j.1365-313X.2010.04217.x

10.1016/j.plaphy.2015.09.008

10.1111/mpp.12057

Lozano‐Torres J.L., 2014, Secreted venom allergen‐like proteins of plant‐parasitic nematodes modulate defence responses in host plants, J. Nematol., 46, 196

10.1371/journal.ppat.1006284

10.1093/nar/29.9.e45

10.1186/s12915-016-0228-7

10.1093/jxb/erx374

10.1111/j.1469-8137.2009.02981.x

10.1111/nph.12535

10.1126/scisignal.2004777

10.1073/pnas.1503657112

10.1111/j.1365-313X.1991.00245.x

10.1023/A:1008609409465

10.1111/j.1365-313X.2008.03727.x

10.1039/c003484c

10.1163/187529267X00120

Wyss U., 1986, Observations on the behavior of second stage juveniles of Heterodera schachtii inside host roots, Rev. Nématol., 9, 153

10.4161/psb.25680

10.1111/nph.13419

Thông tin
Thông tin xuất bản

Molecular Plant Pathology

Tập 19 Số 8

1917-1928

Thông tin tác giả