Aptasensor Based on Screen-Printed Carbon Electrodes Modified with CS/AuNPs for Sensitive Detection of Okadaic Acid in Shellfish

Journal of Analysis and Testing - Tập 7 Số 2 - Trang 128-135 - 2023
Zeng, Ni1, Wang, Xinyi1, Dong, Yiyang1, Yang, Yan1, Yin, Yingai1, Zhao, Lianhui1, Wang, Xu1
1College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China

Tóm tắt

Okadaic acid (OA), a small molecule substance derived from shellfish, is one of the most widely distributed marine toxins with acute symptoms of vomiting and diarrhea after accidental ingestion. For this, there is an urgently need for sensitive and reliable methods to detect OA in real shellfish samples. In this study, a simple aptasensor based on screen-printed carbon electrode (SPCE) with modification of chitosan (CS) and gold nanoparticles (AuNPs) was designed for electrochemical determination of OA, and the electrode surface was modified with AuNPs by potential-sweeping electrodeposition, which greatly improved the electrochemical response. The entire detection and characterization process were carried out by cyclic voltammetry (CV) with a linear correlation in the range of 0.01–100 ng/mL and a limit of detection (LOD) of 6.7 pg/mL. Furthermore, recovery rates of 92.3–116% were obtained demonstrating excellent accuracy through the recovery trial of mussel and scallop samples.

Tài liệu tham khảo

citation_journal_title=Curr Opin Infect Dis; citation_title=Food poisoning associated with biotoxins in fish and shellfish; citation_author=MM Brett; citation_volume=16; citation_publication_date=2003; citation_pages=461-465; citation_doi=10.1097/00001432-200310000-00013; citation_id=CR1 citation_journal_title=Clin Infect Dis; citation_title=Illnesses caused by marine toxins; citation_author=J Sobel, J Painter; citation_volume=41; citation_publication_date=2005; citation_pages=1290-1296; citation_doi=10.1086/496926; citation_id=CR2 citation_journal_title=Cell Physiol Biochem; citation_title=Absorption and effect of azaspiracid-1 over the human intestinal barrier; citation_author=P Abal, MC Louzao, M Fraga, N Vilarino, S Ferreiro, MR Vieytes, LM Botana; citation_volume=43; citation_issue=1; citation_publication_date=2017; citation_pages=136-146; citation_doi=10.1159/000480331; citation_id=CR3 citation_journal_title=Toxicon; citation_title=Marine toxins and nephrotoxicity: mechanism of injury; citation_author=V Sitprija, S Sitprija; citation_volume=161; citation_publication_date=2019; citation_pages=44-49; citation_doi=10.1016/j.toxicon.2019.02.012; citation_id=CR4 citation_journal_title=Chemosphere; citation_title=Chronic exposure to microcystin-LR increases the risk of prostate cancer and induces malignant transformation of human prostate epithelial cells; citation_author=C Pan, L Zhang, X Meng, H Qin, Z Xiang, W Gong, W Luo, D Li, X Han; citation_volume=263; citation_publication_date=2021; citation_doi=10.1016/j.chemosphere.2020.128295; citation_id=CR5 citation_journal_title=Toxicon; citation_title=Okadaic acid (OA): toxicity, detection and detoxification; citation_author=LL Fu, XY Zhao, LD Ji, J Xu; citation_volume=160; citation_publication_date=2019; citation_pages=1-7; citation_doi=10.1016/j.toxicon.2018.12.007; citation_id=CR6 citation_journal_title=J Cancer Res Clin Oncol; citation_title=The concept of the okadaic acid class of tumor promoters is revived in endogenous protein inhibitors of protein phosphatase 2A, SET and CIP2A, in human cancers; citation_author=H Fujiki, E Sueoka, T Watanabe, M Suganuma; citation_volume=144; citation_issue=12; citation_publication_date=2018; citation_pages=2339-2349; citation_doi=10.1007/s00432-018-2765-7; citation_id=CR7 citation_journal_title=Toxins; citation_title=Toxins of okadaic acid-group increase malignant properties in cells of colon cancer; citation_author=D Jimenez-Carcamo, C Garcia, HR Contreras; citation_volume=12; citation_issue=3; citation_publication_date=2020; citation_pages=179; citation_doi=10.3390/toxins12030179; citation_id=CR8 citation_journal_title=J Cell Biochem; citation_title=Okadaic acid promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells by inhibiting protein phosphatase 2A; citation_author=J Kong, D Li, S Zhang, H Zhang, Y Fu, B Qian, C Bei, S Tan, X Zhu; citation_volume=122; citation_publication_date=2021; citation_pages=993-1002; citation_doi=10.1002/jcb.29629; citation_id=CR9 Anon. Regulation (EC) No. 853/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific hygiene rules for food of animal origin. 2004. citation_journal_title=J Vet Med Sci; citation_title=Comparative toxicity of dinophysistoxin-1 and okadaic acid in mice; citation_author=H Suzuki, Y Okada; citation_volume=80; citation_issue=4; citation_publication_date=2018; citation_pages=616-619; citation_doi=10.1292/jvms.17-0377; citation_id=CR11 citation_journal_title=J AOAC Int; citation_title=The laboratory mouse in routine food safety testing for marine algal biotoxins and harmful algal bloom toxin research: past, present and future; citation_author=I Stewart, C McLeod; citation_volume=97; citation_issue=2; citation_publication_date=2014; citation_pages=356-372; citation_doi=10.5740/jaoacint.SGEStewart; citation_id=CR12 citation_journal_title=Toxicon; citation_title=Short-time cytotoxicity of mussel extracts: a new bioassay for okadaic acid detection; citation_author=Z Amzil, YF Pouchus, JL Boterff, C Roussakis, JF Verbist, C Marcaillou-Lebaut, P Masselin; citation_volume=30; citation_issue=11; citation_publication_date=1992; citation_pages=1419-1425; citation_doi=10.1016/0041-0101(92)90517-9; citation_id=CR13 citation_journal_title=J Food Hyg Soc Jpn; citation_title=Validation study for analytical method of diarrhetic shellfish poisons in 9 kinds of shellfish; citation_author=M Yamaguchi, T Yamaguchi, K Kakimoto, H Nagayoshi, M Okihashi, K Kajimura; citation_volume=57; citation_issue=1; citation_publication_date=2016; citation_pages=19-22; citation_doi=10.3358/shokueishi.57.19; citation_id=CR14 citation_journal_title=Czech J Food Sci; citation_title=LC-MS/MS method for the detection of multiple classes of shellfish toxins; citation_author=LJ Chiou, TS Yeh, JC Chen; citation_volume=37; citation_issue=3; citation_publication_date=2019; citation_pages=173-179; citation_doi=10.17221/125/2018-CJFS; citation_id=CR15 citation_journal_title=Food Addit Contam; citation_title=Diarrhetic shellfish poisoning toxin esters in danish blue mussels and surf clams; citation_author=K Jorgensen, S Scanlon, LB Jensen; citation_volume=22; citation_issue=8; citation_publication_date=2005; citation_pages=743-751; citation_doi=10.1080/02652030500136928; citation_id=CR16 citation_journal_title=Toxicon; citation_title=Cross-reactivity of an anti-okadaic acid antibody to dinophysistoxin-4 (DTX-4), dinophysistoxin-5 (DTX-5), and an okadaic acid diol ester; citation_author=JE Lawrence, AD Cembella, NW Ross, JLC Wright; citation_volume=36; citation_issue=8; citation_publication_date=1998; citation_pages=1193-1196; citation_doi=10.1016/S0041-0101(98)00005-1; citation_id=CR17 citation_journal_title=Sci Total Environ; citation_title=Detection of the marine toxin okadaic acid in mussels during a diarrhetic shellfish poisoning (DSP) episode in Thermaikos Gulf, Greece, using biological, chemical and immunological methods; citation_author=T Mouratidou, I Kaniou-Grigoriadou, C Samara, T Kouimtzis; citation_volume=366; citation_issue=2–3; citation_publication_date=2006; citation_pages=894-904; citation_doi=10.1016/j.scitotenv.2005.03.002; citation_id=CR18 citation_journal_title=Anal Biochem; citation_title=A screening lateral flow immunochromatographic assay for on-site detection of okadaic acid in shellfish products; citation_author=SY Lu, C Lin, YS Li, Y Zhou, XM Meng, SY Yu, ZH Li, L Li, HL Ren, ZS Liu; citation_volume=422; citation_issue=2; citation_publication_date=2012; citation_pages=59-65; citation_doi=10.1016/j.ab.2011.12.039; citation_id=CR19 citation_journal_title=Anal Chem; citation_title=Selection and identification of DNA aptamers against okadaic acid for biosensing application; citation_author=S Eissa, A Ng, M Siaj, AC Tavares, M Zourob; citation_volume=85; citation_issue=24; citation_publication_date=2013; citation_pages=11794-11801; citation_doi=10.1021/ac402220k; citation_id=CR20 citation_journal_title=Sens Actuators B Chem; citation_title=From signal amplification to restrained background: Magnetic graphene oxide assisted homogeneous electrochemiluminescence aptasensor for highly sensitive detection of okadaic acid; citation_author=W Yang, G Zhang, J Ni, Q Wang, Z Lin; citation_volume=327; citation_publication_date=2021; citation_doi=10.1016/j.snb.2020.128872; citation_id=CR21 citation_journal_title=Microchem J; citation_title=Graphene immunosensors for okadaic acid detection in seawater; citation_author=J Antunes, C Justino, JP Costa, S Cardoso, AC Duarte, T Rocha-Santos; citation_volume=138; citation_publication_date=2018; citation_pages=465-471; citation_doi=10.1016/j.microc.2018.01.041; citation_id=CR22 citation_journal_title=Anal Methods; citation_title=A sensing smartphone and its portable accessory for on-site rapid biochemical detection of marine toxins; citation_author=J Fang, X Qiu, Z Wan, Q Zou, K Su, N Hu, P Wang; citation_volume=8; citation_issue=38; citation_publication_date=2016; citation_pages=6895-6902; citation_doi=10.1039/C6AY01384H; citation_id=CR23 citation_journal_title=Toxins; citation_title=Marine toxins detection by biosensors based on aptamers; citation_author=W Ye, T Liu, W Zhang, M Zhu, Z Liu, Y Kong, S Liu; citation_volume=12; citation_issue=1; citation_publication_date=2019; citation_pages=1; citation_doi=10.3390/toxins12010001; citation_id=CR24 citation_journal_title=J Hazard Mater; citation_title=Emerging roles of the aptasensors as superior bioaffinity sensors for monitoring shellfish toxins in marine food chain; citation_author=Y Zhao, L Li, X Yan, L Wang, R Ma, X Qi, S Wang, X Mao; citation_volume=421; citation_publication_date=2021; citation_doi=10.1016/j.jhazmat.2021.126690; citation_id=CR25 citation_journal_title=Science; citation_title=Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase; citation_author=C Tuerk, L Gold; citation_volume=249; citation_issue=4968; citation_publication_date=1990; citation_pages=505-510; citation_doi=10.1126/science.2200121; citation_id=CR26 citation_journal_title=Nature; citation_title=In vitro selection of RNA molecules that bind specific ligands; citation_author=AD Ellington, JW Szostak; citation_volume=346; citation_issue=6287; citation_publication_date=1990; citation_pages=818-822; citation_doi=10.1038/346818a0; citation_id=CR27 citation_journal_title=J Mater Chem B; citation_title=Naphthalimide-gold-based nanocomposite for the ratiometric detection of okadaic acid in shellfish; citation_author=M Verma, M Chaudhary, A Singh, N Kaur, N Singh; citation_volume=8; citation_issue=36; citation_publication_date=2020; citation_pages=8405-8413; citation_doi=10.1039/D0TB01195A; citation_id=CR28 citation_journal_title=Sens Actuators B Chem; citation_title=Electrochemical synthesis of phosphorus and sulfur co-doped graphene quantum dots as efficient electrochemiluminescent immunomarkers for monitoring okadaic acid; citation_author=J Peng, Z Zhao, M Zheng, B Su, X Chen, X Chen; citation_volume=304; citation_publication_date=2020; citation_doi=10.1016/j.snb.2019.127383; citation_id=CR29 citation_journal_title=Mikrochim Acta; citation_title=Fluorometric determination of okadaic acid using a truncated aptamer; citation_author=R Chinnappan, R AlZabn, TA Mir, M Bader, M Zourob; citation_volume=186; citation_issue=7; citation_publication_date=2019; citation_pages=406; citation_doi=10.1007/s00604-019-3517-3; citation_id=CR30 citation_journal_title=J Chin Chem Soc; citation_title=Gold Nanoparticle-electrodeposited electrodes used for p-nitrophenol detection in acidic media: effect of electrodeposition parameters on particle density, size distribution, and electrode performance; citation_author=YY Tang, PY Chen; citation_volume=58; citation_issue=6; citation_publication_date=2011; citation_pages=723-731; citation_doi=10.1002/jccs.201190114; citation_id=CR31 citation_journal_title=Anal Sci; citation_title=An Electrochemical DNA biosensor based on Au-reduced graphene oxide nanocomposite for transgenic event Bt63 detection; citation_author=CM He, YL Tang, S Wang, JH Liu, Y Chen, YY Dong, HJ Su, TW Tan; citation_volume=33; citation_issue=10; citation_publication_date=2017; citation_pages=1155-1160; citation_doi=10.2116/analsci.33.1155; citation_id=CR32 citation_journal_title=Mikrochim Acta; citation_title=An impedimetric aptasensor for Shigella dysenteriae using a gold nanoparticle-modified glassy carbon electrode; citation_author=SS Zarei, S Soleimanian-Zad, AA Ensafi; citation_volume=185; citation_issue=12; citation_publication_date=2018; citation_pages=538; citation_doi=10.1007/s00604-018-3075-0; citation_id=CR33 citation_journal_title=Talanta; citation_title=Automated flow-through amperometric immunosensor for highly sensitive and on-line detection of okadaic acid in mussel sample; citation_author=RB Dominguez, A Hayat, A Sassolas, GA Alonso, R Munoz, JL Marty; citation_volume=99; citation_publication_date=2012; citation_pages=232-237; citation_doi=10.1016/j.talanta.2012.05.045; citation_id=CR34 citation_journal_title=Sens Actuators B Chem; citation_title=Electrochemical impedimetric immunosensor for the detection of okadaic acid in mussel sample; citation_author=A Hayat, L Barthelmebs, JL Marty; citation_volume=171–172; citation_publication_date=2012; citation_pages=810-815; citation_doi=10.1016/j.snb.2012.05.075; citation_id=CR35 citation_journal_title=Biosens Bioelectron; citation_title=Phosphorene-gold nanocomposite based microfluidic aptasensor for the detection of okadaic acid; citation_author=S Ramalingam, R Chand, CB Singh, A Singh; citation_volume=135; citation_publication_date=2019; citation_pages=14-21; citation_doi=10.1016/j.bios.2019.03.056; citation_id=CR36