Aptamer based test stripe for ultrasensitive detection of mercury(II) using a phenylene-ethynylene reagent on nanoporous silver as a chemiluminescence reagent
Tóm tắt
We describe a paper-based chemiluminescence (CL) test for the determination of mercury(II) ion. A single-stranded DNA aptamer was first covalently immobilized via its amino groups to the hydroxy groups on the surface of cellulosic paper. The aptamer probes can capture Hg(II) ions due to their specific interaction with thymine. The CL reagent (a caboxylated phenylene-ethynylene referred to as P-acid) was immobilized on nanoporous silver (NPS@P-acid) and used a CL label on the aptamer. The stripe is then contacted with a sample containing Hg(II) ions and CL is induced by the addition of permanganate. CL intensity depends on the concentration of Hg(II) because Hg(II) increases the quantity of the P-acid-conjugated aptamer. The highly active surface of the NPS@P-acid composites results in an 8-fold higher CL intensity compared to the use of pure P-acid. This enables Hg(II) ion to be quantified in the 20 nM to 0.5 μM concentration range, with a limit of detection as low as 1 pM. This CL aptasensor is deemed to represent a promising tool for simple, rapid, and sensitive detection of Hg(II).
Tài liệu tham khảo
Nolan EM, Lippard SJ (2008) Tools and tactics for the optical detection of mercuric ion. Chem Rev 108:3443–3480
Vupputuri S, Longnecker MP, Daniels JL, Guo X, Sandler DP (2005) Blood mercury level and blood pressure among US women: results from the National Health and Nutrition Examination Survey. Environ Res 97:195–200
Virtanen JK, Rissanen TH, Voutilainen S, Tuomainen TP (2007) Mercury as a risk factor for cardiovascular diseases. J Nutr Biochem 18:75–85
Malinovsky D, Sturgeon RE, Yang L (2008) Anion-exchange chromatographic separation of Hg for isotope ratio measurements by multicollector ICPMS. Anal Chem 80:2548–2555
Nguyen TH, Boman J, Leermakers M, Baeyens W (1998) Mercury analysis in environmental samples by EDXRF and CV-AAS. Fresenius J Anal Chem 360:199–204
Gomez-Ariza JL, Lorenzo F, Garcıa-Barrera T (2005) Comparative study of atomic fluorescence spectroscopy and inductively coupled plasma mass spectrometry for mercury and arsenic multispeciation. Anal Bioanal Chem 382:485–492
Gong J, Zhou T, Song D, Zhang L, Hu X (2010) Monodispersed Au nanoparticles decorated graphene as an enhanced sensing platform for ultrasensitive stripping voltammetric detection of mercury (II). Anal Chem 82:567–573
Tuerk C, Gold L (1990) Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 249:505–510
Ellington AD, Szostak JW (1990) Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures. Nature 346:818–822
Robertson DL, Joyce GF (1990) Selection in vitro of an RNA enzyme that specifically cleaves single-stranded DNA. Nature 344:467–468
Miyake Y, Togashi H, Tashiro M, Yamaguchi H, Oda S, Kudo M, Tanaka Y, Kondo Y, Sawa R, Fujimoto T, Machinami T, Ono A (2006) MercuryII-mediated formation of thymine-HgII-thymine base pairs in DNA duplexes. J Am Chem Soc 128:2172–2173
Pelossof G, Tel-Vered R, Liu XQ, Willner I (2011) Amplified surface plasmon resonance based DNA biosensors, aptasensors, and Hg2+ sensors using hemin/G-quadruplexes and Au nanoparticles. Chem Eur J 17:8904–8912
Li D, Wieckowska A, Willner I (2008) Optical analysis of Hg2+ ions by oligonucleotide–gold-nanoparticle hybrids and DNA-based machines. Angew Chem Int Ed 47:3927–3931
Kim JS, Quang DT (2007) Calixarene-derived fluorescent probes. Chem Rev 107:3780–3799
Zuo X, Song S, Zhang J, Pan D, Wang L, Fan CA (2007) A target-responsive electrochemical aptamer switch (TREAS) for reagentless detection of nanomolar ATP. J Am Chem Soc 129:1042–1043
Martinez AW, Phillips ST, Butte MJ, Whitesides GM (2007) Patterned paper as a platform for inexpensive, low-volume, portable bioassays. Angew Chem Int Ed 46:1318–1320
Martinez AW, Phillips ST, Carrilho E, Thomas SW III, Sindi H, Whitesides GM (2008) Three-dimensional microfluidic devices fabricated in layered paper and tape. Anal Chem 80:3699–3707
Bruzewicz DA, Reches M, Whitesides GM (2008) Low-cost printing of poly (dimethylsiloxane) barriers to define microchannels in paper. Anal Chem 80:3387–3392
Carrilho E, Martinez AW, Whitesides GM (2009) Understanding wax printing: a simple micropatterning process for paper-based microfluidics. Anal Chem 81:7091–7095
Li X, Tian J, Nguyen T, Shen W (2008) Paper-based microfluidic devices by plasma treatment. Anal Chem 80:9131–9134
Chitnis G, Ding Z, Chang CL, Savran CA, Ziaie B (2011) Laser-treated hydrophobic paper: an inexpensive microfluidic platform. Lab Chip 11:1161–1165
Wang SM, Ge L, Song XR, Yu JH, Ge SG, Huang JD, Zeng F (2012) Paper-based chemiluminescence ELISA: Lab-on-paper based on chitosan modified paper device and wax-screen-printing. Biosens Bioelectron 31:212–218
Xu CX, Li YY, Tian F, Ding Y (2010) Dealloying to nanoporous silver and its implementation as a template material for construction of nanotubular mesoporous bimetallic nanostructures. Chem Phys Chem 11:3320–3328
Xu CX, Liu YQ, Su F, Liu AH, Qiu HJ (2011) Nanoporous PtAg and PtCu alloys with hollow ligaments for enhanced electrocatalysis and glucose biosensing. Biosens Bioelectron 27:160–166
Yan M, Ge L, Gao WQ, Yu JH, Song XR, Ge SG, Jia ZY, Chu CC (2012) Electrogenerated chemiluminescence from a phenyleneethynylene derivative and its ultrasensitive immunosensing application using a nanotubular mesoporous Pt–Ag alloy for signal amplification. Adv Funct Mater 22:3899–3906
Hermanson GT (2008) Bioconjugate techniques, 2nd edn. Elsevier, Rockford
Noh HB, Rahman MA, Yang JE, Shim YB (2011) Ag (I)-cysteamine complex based electrochemical stripping immunoassay: ultrasensitive human IgG detection. Biosens Bioelectron 26:4429–4435
Wang SM, Ge L, Song XR, Yan M, Ge SG, Yu JH, Zeng F (2012) Simple and covalent fabrication of a paper device and its application in sensitive chemiluminescence immunoassay. Analyst 137:3821–3827
Tan YH, Davis JA, Fujikawa K, Ganesh NV, Demchenko AV, Stine KJ (2012) Surface area and pore size characteristics of nanoporous gold subjected to thermal, mechanical, or surface modification studied using gas adsorption isotherms, cyclic voltammetry, thermogravimetric analysis, and scanning electron microscopy. J Mater Chem 22:6733–6745
Yuan C, Zhang K, Zhang Z, Wang S (2012) Highly selective and sensitive detection of mercuric ion based on a visual fluorescence method. Anal Chem 84:9792–9801
Du J, Liu M, Lou X, Zhao T, Wang Z, Xue Y, Zhao J, Xu Y (2012) Highly sensitive and selective chip-based fluorescent sensor for mercuric ion: development and comparison of turn-on and turn-off systems. Anal Chem 84:8060–8066
Huang D, Niu C, Wang X, Lv X, Zeng G (2013) “Turn-on” fluorescent sensor for Hg2+ based on single-stranded DNA functionalized Mn: CdS/ZnS quantum dots and gold nanoparticles by time-gated mode. Anal Chem 85:1164–1170
Han D, Kim Y, Oh JW, Kim TH, Mahajan RK, Kim JS, Kim H (2009) A regenerative electrochemical sensor based on oligonucleotide for the selective determination of mercury (II). Analyst 134:1857–1862
Aragay G, Pons J, Merkoc A (2011) Enhanced electrochemical detection of heavy metals at heated graphite nanoparticle-based screen-printed electrodes. J Mater Chem 21:4326–4331