Application of λ Red recombination system to Vibrio cholerae genetics: Simple methods for inactivation and modification of chromosomal genes

Beloin, 2003, An extended role for the nucleoid structuring protein H-NS in the virulence gene regulatory cascade of Shigella flexneri, Mol. Microbiol., 47, 825, 10.1046/j.1365-2958.2003.03347.x Camerini-Otero, 1995, Homologous recombination proteins in prokaryotes and eukaryotes, Annu. Rev. Genet., 29, 509, 10.1146/annurev.genet.29.1.509 Cherepanov, 1995, Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant, Gene, 158, 9, 10.1016/0378-1119(95)00193-A Chiang, 2000, Construction of a Vibrio cholerae vaccine candidate using transposon delivery and FLP recombinase-mediated excision, Infect. Immun., 68, 6391, 10.1128/IAI.68.11.6391-6397.2000 Datsenko, 2000, One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products, Proc. Natl. Acad. Sci. U S A., 97, 6640, 10.1073/pnas.120163297 Datta, 2006, A set of recombineering plasmids for Gram-negative bacteria, Gene, 379, 109, 10.1016/j.gene.2006.04.018 Datta, 2008, Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages, Proc. Natl. Acad. Sci. U S A., 105, 1626, 10.1073/pnas.0709089105 Derbise, 2003, A rapid and simple method for inactivating chromosomal genes in Yersinia, FEMS Immunol. Med. Microbiol., 38, 113, 10.1016/S0928-8244(03)00181-0 DiRita, 1991, Regulatory cascade controls virulence in Vibrio cholerae, Proc. Natl. Acad. Sci. U S A., 88, 5403, 10.1073/pnas.88.12.5403 Heidelberg, 2000, DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae, Nature, 406, 477, 10.1038/35020000 Husseiny, 2005, Rapid method for the construction of Salmonella enterica serovar Typhimurium vaccine carrier strains, Infect Immun., 73, 1598, 10.1128/IAI.73.3.1598-1605.2005 Iwanaga, 1986, Culture conditions for stimulating cholera toxin production by Vibrio cholerae O1 El Tor, Microbiol. Immunol., 30, 1075, 10.1111/j.1348-0421.1986.tb03037.x Janes, 2001, Growth inhibition caused by overexpression of the structural gene for glutamate dehydrogenase (gdhA) from Klebsiella aerogenes, J. Bacteriol., 183, 2709, 10.1128/JB.183.8.2709-2714.2001 Lesic, 2008, Use of the lambda Red recombinase system to rapidly generate mutants in Pseudomonas aeruginosa, BMC Mol Biol., 9, 20, 10.1186/1471-2199-9-20 Lockman, 1983, Nucleotide sequence analysis of the A2 and B subunits of Vibrio cholerae enterotoxin, J. Biol. Chem., 258, 13722, 10.1016/S0021-9258(17)43977-9 Malo, 1988, Promoter-detection vectors for Escherichia coli with multiple useful features, Gene, 64, 207, 10.1016/0378-1119(88)90336-8 Miller, 1972 Miller, 1988, A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR, J. Bacteriol., 170, 2575, 10.1128/jb.170.6.2575-2583.1988 Rossi, 2003, Haemophore-mediated signal transduction across the bacterial cell envelope in Serratia marcescens: the inducer and the transported substrate are different molecules, Mol. Microbiol., 48, 1467, 10.1046/j.1365-2958.2003.03516.x Sambrook, 1989 Unger, 1972, Interaction of the recombination pathways of bacteriophage lambda and its host Escherichia coli K12: effects on exonuclease V activity, J. Mol. Biol., 70, 539, 10.1016/0022-2836(72)90558-X Unger, 1972, Interaction of the recombination pathways of bacteriophage lambda and its host Escherichia coli K12: effects on lambda recombination, J. Mol. Biol., 70, 531, 10.1016/0022-2836(72)90557-8 Yu, 2000, An efficient recombination system for chromosome engineering in Escherichia coli, Proc. Natl. Acad. Sci. U S A., 97, 5978, 10.1073/pnas.100127597 Yu, 2002, Regulation of gene expression in Vibrio cholerae by ToxT involves both antirepression and RNA polymerase stimulation, Mol. Microbiol., 43, 119, 10.1046/j.1365-2958.2002.02721.x