Application and evaluation of the alamarblue assay for cell growth and survival of fibroblasts

Sherry L. Voytik‐Harbin1, Andrew O. Brightman1, Beverly Waisner2, Carlton H. Lamar2, Stephen F. Badylak1
1Hillenbrand Biomedical Engineering Center, Purdue University, West Lafayette
2Department of Basic Medical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette

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Ahmed, S. A.; Gogal, R. M.; Walsh, J. E. A new rapid and simple nonradioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H]thymidine incorporation assay. J Immunol. Methods 170:211–224; 1994.

Alley, M. C.; Scudiero, D. A.; Monks, A., et al. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res. 48:589–601; 1988.

Baker, C. N.; Banerjee, S. N.; Tenover, F. C. Evaluation of alamar colorimetric MIC method for antimicrobacterial susceptibility testing of gram-negative bacteria. J. Clin. Microbiol. 32:1261–1267; 1994.

de Fries, R.; Mitsuhashi, M. Quantification of mitogen induced human lymphocyte proliferation: comparison of alamarBlue assay to [3H]thymidine incorporation assay. J. Clin. Lab. Anal. 9:89–95; 1995.

Denizot, F.; Lang, R. Rapid colorimetric assay for cell growth and survival. J. Immunol. Methods 89:271–277; 1986.

Espevik, T.; Nissen-Meyer, J. A highly sensitive cell line, WEHI 164 clone 13, for measuring cytotoxic factor/tumor necrosis factor from human monocytes. J. Immunol. Methods 95:99–105; 1986.

Filman, D. J.; Brawn, R. J.; Dandliker, W. B. Intracellular supravital stain delocalization as an assay for antibody-dependent complement-mediated cell damage. J. Immunol. Methods 6:189–207; 1975.

Flick, D. A.; Gifford, G. E. J. Comparison of in vitro cell cytotoxic assays for tumor necrosis factor. J. Immunol. Methods 68:167–175; 1984.

Gillies, R. J.; Didier, N.; Denton, M. Determination of cell number in monolayer cultures. Anal. Biochem. 159:109–113; 1986.

Green, L. M.; Read, J. L.; Ware, C. F. Rapid colorimetric assay for cell viability: application to the quantification of cytotoxic and growth inhibitory lymphokines. J. Immunol. Methods 70:257–268; 1984.

Hansen, M. B.; Nielsen, S. E.; Berg, K. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol. Methods 119:203–210; 1989.

Heeg, K.; Reimann, J.; Kabelitz, D., et al. A rapid colorimetric assay for the determination of IL-2-producing helper T cell frequencies. J. Immunol. Methods 77:237–246; 1985.

Honn, K. V.; Singley, J. A.; Chavin, W. fetal bovine serum: a multivariate standard. Proc. Soc. Exp. Biol. Med. 149:344–347; 1975.

Jabbar, S. A. B.; Twentyman, P. R.; Watson, J. V. The MTT assay underestimates the growth inhibitory effects of interferons. Br. J. Cancer 60:523–528; 1989.

Lowik, C. W. G. M.; Alblas, M. J.; van de Ruit, M., et al. Quantification of adherent and nonadherent cells cultured in 96-well plates using the supravital stain neutral red. Anal. Biochem. 213:426–433; 1993.

Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytoxicity assays. J. Immunol. Methods 65:55–63; 1983.

Neville, M. E. Cr-uptake assay. A sensitive and reliable method to quantitate cell viability and cell death. J. Immunol. Methods 99:77–82; 1987.

Novak, S. M.; Hindler, J.; Bruckner, D. A. Reliability of two novel methods, alamar and E test, for detection of methicillin-resistant staphylococcus aureus. J. Clin. Microbiol. 31:3056–3057; 1993.

Oliver, M. H.; Harrison, N. K.; Bishop, J. E., et al. A rapid and convenient assay for counting cells cultured in microwell plates: application for assessment of growth factors. J. Cell Sci. 92:513–518; 1989.

Olmsted, C. A. A physico-chemical study of fetal calf sera used as tissue culture nutrient correlated with biological tests for toxicity. Exp. Cell Res. 48:283–299; 1967.

Page, B.; Page, M.; Noel, C. A new fluorometric assay for cytotoxicity measurements in vitro. Int. J. Oncol. 3:473–476; 1993.

Pfaller, M. A.; Grant, C.; Morthland, V., et al. Comparative evaluation of alternative methods for broth dilution susceptibility testing for fluconazole against candida albicans. J. Clin. Microbiol. 32:506–509; 1994.

Plumb, J. A.; Milroy, R.; Kaye, S. B. Effects of the pH dependence of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-formazan absorption on chemoselectivity determined by novel tetrazolium-based assay. Cancer Res. 49:4435–4440; 1989.

Slater, T. F.; Sawyer, B.; Strauli, U. Studies on succinate-tetrazolium reductase systems III. Points of coupling of four different tetrazolium salts. Biochim. Biophys. Acta 77:383–393; 1963.

Tada, H.; Shibo, O.; Kuroshima, K., et al. An improved colorimetric assay for interleukin-2. J. Immunol. Methods 93:157–165; 1986.

Vistica, D. T.; Skehan, P.; Scudiero, D., et al. Tetrazolium-based assays for cellular viability: a critical examination of selected parameters affecting formazan production. Cancer Res. 51:2515–2520; 1991.