Antioxidative properties and inhibitory effect of Bifidobacterium adolescentis on melanogenesis

World Journal of Microbiology and Biotechnology - Tập 28 - Trang 2903-2912 - 2012
Huey-Chun Huang1, Tsong-Min Chang2
1Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan
2Department of Applied Cosmetology and Master Program of Cosmetic Science, Hung Kuang University, Shalu, Taichung County, Taiwan, ROC

Tóm tắt

Melanin is a dark pigment produced by melanocytes. Tyrosinase is a key enzyme which catalyzes the rate-limiting step of melanogenesis. However, accumulation of melanin leads to various skin hyperpigmentation disorders. To find a novel skin-whitening agent, the antioxidant capacity of Bifidobacterium adolescentis culture filtrate and inhibitory effect on melanogenesis were investigated. The antioxidant effects of B. adolescentis culture filtrate include 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging capacity, 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid)(ABTS) radical cation scavenging activity and reducing power were measured spectrophotometrically. The reducing power is a useful index for the evaluation of potential antioxidants which carry out reduction of ferricyanide to ferrocyanide. Furthermore, the inhibitory effects of the bacterial culture filtrate on mushroom tyrosinase, B16F10 intracellular tyrosinase activity and melanin content were also determined. The results revealed that B. adolescentis culture filtrate (2.5, 5.0 and 7.5 %; v/v) effectively scavenged DPPH and ABTS radicals, and lower concentrations of the bacterial culture filtrates (0.5, 1.0 and 1.5 %; v/v) showed potent reducing power in a dose-dependent pattern. Additionally, the bacterial culture filtrate suppressed murine tyrosinase activity and decreased the amount of melanin in a dose-dependent manner. Our results demonstrated that B. adolescentis culture filtrate decreases the melanogenesis process of melanoma cells by inhibiting tyrosinase activity, which we suggest may be mediated through its antioxidant activity.

Tài liệu tham khảo

Bilodeau ML, Greulich JD, Hullinger RL, Bertolotto C, Ballotti R, Andrisani OM (2001) BMP-2 stimulates tyrosinase gene expression and melanogenesis in differentiated melanocytes. Pigment Cell Res 14:328–336 Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 28:25–30 Briganti S, Camera E, Picardo M (2003) Chemical and instrumental approaches to treat hyperpigmentation. Pigment Cell Res 16:101–110 del Marmol V, Solano F, Sels A, Huez G, Libert A, Lejeune F, Ghanem G (1993) Glutathione depletion increases tyrosinase activity in human melanoma cells. J Investig Dermatol 101:871–874 Emerit I (1992) Free radicals and aging of the skin. EXS 62:328–341 Femia AP, Luceri C, Dolara P, Giannini A, Biggeri A, Salvadori M, Clune Y, Collins KJ, Paglierani M, Caderni G (2002) Antitumorigenic activity of the prebiotic inulin enriched with oligofructose in combination with the probiotics Lactobacillus rhamnosus and Bifidobacterium lactis on azoxymethane-induced colon carcinogenesis in rats. Carcinogenesis 23:1953–1960 Funasaka Y, Komoto M, Ichihashi M (2000) Depigmenting effect of alpha-tocopheryl ferulate on normal human melanocytes. Pigment Cell Res 13(Suppl 8):170–174 Galván I, Alonso-Alvarez C (2008) An intracellular antioxidant determines the expression of a melanin-based signal in a bird. PLoS ONE 3:e3335 Gibson GR, Wang X (1994) Regulatory effects of bifidobacteria on the growth of other colonic bacteria. J Appl Bacteriol 77:412–420 Gilliland SE (1990) Health and nutritional benefits from lactic acid bacteria. FEMS Microbiol Lett 87:175–188 Halliwell B, Gutteridge JM, Cross CE (1992) Free radicals, antioxidants, and human disease: where are we now? J Lab Clin Med 119:598–620 Hearing VJ, Jimenez M (1987) Mammalian tyrosinase–the critical regulatory control point in melanocyte pigmentation. Int J Biochem 19:1141–1147 Hooper LV, Gordon JI (2001) Commensal host-bacterial relationships in the gut. Science 292:1115–1118 Huang H, Chiu SH, Ke HJ, Chiu SW, Wu SY, Chang TM (2011a) Antimelanogenic and antioxidant activities of Bifidobacterium infantis. Af J Microbiol Res 5:3150–3156 Huang HC, Huang WY, Chiu SH, Ke HJ, Chiu SW, Wu SY, Kuo FS, Chang TM (2011b) Antimelanogenic and antioxidative properties of Bifidobacterium bifidum. Arch Dermatol Res 303:527–531 Huang HC, Chang TY, Chang LZ, Wang HF, Yih KH, Hsieh WY, Chang TM (2012) Inhibition of melanogenesis versus antioxidant properties of essential oil extracted from leaves of vitex negundo linn and chemical composition analysis by GC-MS. Molecules 17:3902–3916 Imokawa G (1989) Analysis of initial melanogenesis including tyrosinase transfer and melanosome differentiation through interrupted melanization by glutathione. J Invest Dermatol 93:100–107 Jimenez-Cervantes C, Solano F, Kobayashi T, Urabe K, Hearing VJ, Lozano JA, Garcia-Borron JC (1994) A new enzymatic function in the melanogenic pathway. The 5,6-dihydroxyindole-2-carboxylic acid oxidase activity of tyrosinase-related protein-1 (TRP1). J Biol Chem 269:17993–18000 Kalliomaki M, Salminen S, Arvilommi H, Kero P, Koskinen P, Isolauri E (2001) Probiotics in primary prevention of atopic disease: a randomised placebo-controlled trial. Lancet 357:1076–1079 Kalliomaki M, Salminen S, Poussa T, Arvilommi H, Isolauri E (2003) Probiotics and prevention of atopic disease: 4-year follow-up of a randomised placebo-controlled trial. Lancet 361:1869–1871 Kalliomaki M, Salminen S, Poussa T, Isolauri E (2007) Probiotics during the first 7 years of life: a cumulative risk reduction of eczema in a randomized, placebo-controlled trial. J Allergy Clin Immunol 119:1019–1021 Karg E, Odh G, Wittbjer A, Rosengren E, Rorsman H (1993) Hydrogen peroxide as an inducer of elevated tyrosinase level in melanoma cells. J Invest Dermatol 100:209S–213S Kim Y, Lee D, Kim D, Cho J, Yang J, Chung M, Kim K, Ha N (2008) Inhibition of proliferation in colon cancer cell lines and harmful enzyme activity of colon bacteria by < i>Bifidobacterium adolescentis SPM0212. Arch Pharm Res 31:468–473 Kumano Y, Sakamoto T, Egawa M, Iwai I, Tanaka M, Yamamoto I (1998) In vitro and in vivo prolonged biological activities of novel vitamin C derivative, 2-O-alpha-D-glucopyranosyl-L-ascorbic acid (AA-2G), in cosmetic fields. J Nutr Sci Vitaminol (Tokyo) 44:345–359 Lee D, Jang S, Kim M, Kim J, Chung M, Kim K, Ha N (2008) Anti-proliferative effects of Bifidobacterium adolescentis SPM0212 extract on human colon cancer cell lines. BMC Cancer 8:310 Ouwehand A, Isolauri E, Salminen S (2002). The role of the intestinal microflora for the development of the immune system in early childhood. Eur J Nutr 41(Suppl 1):132–137 Ouwehand AC, Batsman A, Salminen S (2003) Probiotics for the skin: a new area of potential application? Lett Appl Microbiol 36:327–331 Oyaizu M (1986) Studies on products of browing reaction: antioxidative activity of product of browing reaction preapared from glucosamine. Jpn J Nutr 44:307–315 Puch F, Samson-Villeger S, Guyonnet D, Blachon JL, Rawlings AV, Lassel T (2008) Consumption of functional fermented milk containing borage oil, green tea and vitamin E enhances skin barrier function. Exp Dermatol 17:668–674 Rautava S, Isolauri E (2002) The development of gut immune responses and gut microbiota: effects of probiotics in prevention and treatment of allergic disease. Curr Issues Intest Microbiol 3:15–22 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231–1237 Rodrigues KL, Caputo LR, Carvalho JC, Evangelista J, Schneedorf JM (2005) Antimicrobial and healing activity of kefir and kefiran extract. Int J Antimicrob Agents 25:404–408 Ryu A, Arakane K, Koide C, Arai H, Nagano T (2009) Squalene as a Target Molecule in Skin Hyperpigmentation Caused by Singlet Oxygen. Biol Pharm Bull 32:1504–1509 Sánchez-Moreno C, Larrauri JA, Saura-Calixto F (1998) A procedure to measure the antiradical efficiency of polyphenols. J Sci Food Agric 76:270–276 Seo SY, Sharma VK, Sharma N (2003) Mushroom tyrosinase: recent prospects. J Agric Food Chem 51:2837–2853 Strober W (2001) Current Protocols in Immunology. Wiley, Baltimore Tada H, Shiho O, Kuroshima K, Koyama M, Tsukamoto K (1986) An improved colorimetric assay for interleukin 2. J Immunol Methods 93:157–165 Tsuboi T, Kondoh H, Hiratsuka J, Mishima Y (1998) Enhanced melanogenesis induced by tyrosinase gene-transfer increases boron-uptake and killing effect of boron neutron capture therapy for amelanotic melanoma. Pigment Cell Res 11:275–282 Tsukamoto K, Jackson IJ, Urabe K, Montague PM, Hearing VJ (1992) A second tyrosinase-related protein, TRP-2, is a melanogenic enzyme termed DOPAchrome tautomerase. EMBO J 11:519–526 Yamakoshi J, Otsuka F, Sano A, Tokutake S, Saito M, Kikuchi M, Kubota Y (2003) Lightening effect on ultraviolet-induced pigmentation of guinea pig skin by oral administration of a proanthocyanidin-rich extract from grape seeds. Pigment Cell Res 16:629–638 Yang JY, Koo JH, Song YG, Kwon KB, Lee JH, Sohn HS, Park BH, Jhee EC, Park JW (2006) Stimulation of melanogenesis by scoparone in B16 melanoma cells. Acta Pharmacol Sin 27:1467–1473 Yasui H, Sakurai H (2003) Age-dependent generation of reactive oxygen species in the skin of live hairless rats exposed to UVA light. Exp Dermatol 12:655–661