Antioxidant capacities of the selenium nanoparticles stabilized by chitosan
Tóm tắt
Selenium (Se) as one of the essential trace elements for human plays an important role in the oxidation reduction system. But the high toxicity of Se limits its application. In this case, the element Se with zero oxidation state (Se0) has captured our attention because of its low toxicity and excellent bioavailability. However, Se0 is very unstable and easily changes into the inactive form. By now many efforts have been done to protect its stability. And this work was conducted to explore the antioxidant capacities of the stable Se0 nanoparticles (SeNPs) stabilized using chitosan (CS) with different molecular weights (Mws) (CS-SeNPs). The different Mws CS-SeNPs could form uniform sphere particles with a size of about 103 nm after 30 days. The antioxidant tests of the DPPH, ABTS, and lipid peroxide models showed that these CS-SeNPs could scavenge free radicals at different levels. And the 1 month old SeNPs held the higher ABTS scavenging ability that the value could reach up to 87.45 ± 7.63% and 89.44 ± 5.03% of CS(l)-SeNPs and CS(h)-SeNPs, respectively. In the cell test using BABLC-3T3 or Caco-2, the production of the intracellular reactive oxygen species (ROS) could be inhibited in a Se concentration-dependent manner. The topical or oral administration of CS-SeNPs, particularly the Se nanoparticles stabilized with low molecular weight CS, CS(l)-SeNPs, and treated with a 30-day storage process, could efficiently protect glutathione peroxidase (GPx) activity and prevent the lipofusin formation induced by UV-radiation or d-galactose in mice, respectively. Such effects were more evident in viscera than in skin. The acute toxicity of CS(l)-SeNPs was tenfold lower than that of H2SeO3. Our work could demonstrate the CS-SeNPs hold a lower toxicity and a 30-day storage process could enhance the antioxidant capacities. All CS-SeNPs could penetrate the tissues and perform their antioxidant effects, especially the CS(l)-SeNPs in mice models. What’s more, the antioxidant capacities of CS-SeNPs were more evident in viscera than in skin.
Tài liệu tham khảo
Tapiero H, Townsend DM, Tew KD. The antioxidant role of selenium and seleno-compounds. Biomed Pharmacother. 2003;57:134–44.
Liu W, Zheng WJ, Zhang YB, Cao WQ, Chen TF. Selenium nanoparticles as a carrier of 5-fluorouracil to achieve anticancer synergism. ACS Nano. 2012;6:6578–91.
McKenzie RC. Selenium, ultraviolet radiation and the skin. Clin Exp Dermatol. 2000;25:631–6.
Qin S, et al. Effects of selenium-chitosan on blood selenium concentration, antioxidation status, and cellular and humoral immunity in mice. Biol Trace Elem Res. 2015;165:145–52.
Wang WF, et al. Dietary selenium requirement and its toxicity in juvenile abalone Haliotis discus hannai Ino. Aquaculture. 2012;330:42–6.
Wang H, Zhang J, Yu H. Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: comparison with selenomethionine in mice. Free Radic Biol Med. 2007;42:1524–33.
Torres SK, et al. Biosynthesis of selenium nanoparticles by Pantoea agglomerans and their antioxidant activity. J Nanopart Res. 2012;14:1–9.
Zhang JS, Wang HL, Yan XX, Zhang LD. Comparison of short-term toxicity between Nano-Se and selenite in mice. Life Sci. 2005;10:1099–109.
Chen HY, Shin DW, Nam JG, Kwon KW, Yoo JB. Selenium nanowires and nanotubes synthesized via a facile template-free solution method. Mater Res Bull. 2010;45:699–704.
Rai M, Yadav A, Gade A. Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv. 2009;27:76–83.
Daniel MC, Astruc D. Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chem Rev. 2004;104:293–346.
Schubert D, Dargusch R, Raitano J, Chan SW. Cerium and yttrium oxide nanoparticles are neuroprotective. Biochem Biophys Res Commun. 2006;342:86–91.
Kim J, et al. Effects of a potent antioxidant, platinum nanoparticle, on the lifespan of Caenorhabditis elegans. Mech Aging Dev. 2008;129:322–31.
Peters RJB, et al. Nanomaterials for products and application in agriculture, feed and food. Trends Food Sci Technol. 2016;54:155–64.
Kaur G, Iqbal M, Bakshi MS. Biomineralization of fine selenium crystalline rods and amorphous spheres. J Phys Chem C. 2009;113:13670–6.
Zhang YF, Wang JG, Zhang LN. Creation of highly stable selenium nanoparticles capped with hyperbranched polysaccharide in water. Langmuir. 2010;26:17617–23.
Wu SS, Sun K, Wang X, Wang DX, Wan XC, Zhang JS. Protonation of epigallocatechin-3-gallate (EGCG) results in massive aggregation and reduced oral bioavailability of EGCG-dispersed selenium nanoparticles. J Arg Food Chem. 2013;61:7268–75.
Estevez H, Garcia-Lidon JC, Luque-Garcia JL, Cmara C. Effects of chitosan-stabilized selenium nanoparticles on cell proliferation, apoptosis and cell cycle pattern in HepG2 cells: comparison with other selenospecies. Colloid Surf B. 2014;122:184–93.
Anal AK, Stevens WF, Remuñán-López C. Ionotropic cross-linked chitosan microspheres for controlled release of ampicillin. Int J Pharm. 2006;312:166–73.
Roncal T, Oviedo A, Armentia IL, Fernández DL, Villarán MC. High yield production of monomer-free chitosan oligosaccharides by pepsin catalyzed hydrolysis of a high deacetylation degree chitosan. Carbohyd Res. 2007;342:2750–6.
Zhang CY, Zhai XN, Zhao GH, Ren FZ, Leng XJ. Synthesis, characterization, and controlled release of selenium nanoparticles stabilized by chitosan of different molecular weights. Carbohyd Polym. 2015;134:158–66.
Blagosklonny MV. Aging: Ros or tor. Cell Cycle. 2008;7:3344–54.
Brunk UT, Terman A. Lipofuscin: mechanisms of age-related accumulation and influence on cell function. Free Radic Biol Med. 2002;33:611–9.
Family F, Mazzitello KI, Arizmendi CM, Grossniklaus HE. Dynamic scaling of lipofuscin deposition in aging cells. J Stat Phys. 2011;144:332–43.
Höhn A, König J, Grune T. Protein oxidation in aging and the removal of oxidized proteins. J Proteom. 2013;92:132–59.
Terman A, Brunk ULFT. Lipofuscin: mechanisms of formation and increase with age. Apmis. 1998;106:265–76.
Thorling EB, Overvad K, Bjerring P. Oral selenium inhibits skin reactions to UV light in hairless mice. Acta Pathologica Microbiologica Scandinavica Series A: Pathol. 1983;91:81–4.
Overvad K, Thorling EB, Bjerring P, Rbbesen P. Selenium inhibits UV-light-induced skin carcinogenesis in hairless mice. Cancer Lett. 1985;27:163–70.
Burke KE, Combs-Jr GF, Gross EG, Bhuyan KC, Abu-Lideh H. The effects of topical and oral l-selenomethionine on pigmentation and skin cancer induced by ultraviolet irradiation. Nutr Cancer. 1992;2:123–37.
Lynch AM, Wilcox P. Review of the performance of the 3T3 NRU in vitro phototoxicity assay in the pharmaceutical industry. Exp Toxicol Pathol. 2011;63:209–14.
Xu BJ, Chang SKC. A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J Food Sci. 2007;72:S159–66.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26:1231–7.
Tsuda T, et al. Antioxidative activity of the anthocyanin pigments cyanidin 3-O-.β-d-glucoside and cyaniding. J Agric Food Chem. 1994;42:2407–10.
Wolfe KL, Hai LR. Cellular antioxidant activity (CAA) assay for assessing antioxidants, foods, and dietary supplements. J Agric Food Chem. 2007;55:8896–907.
Hamilton MA, Russo RC, Thurston RV. Trimmed Spearman-Karber method for estimating median lethal concentrations in toxicity bioassays. Environ Sci Technol. 1977;11:714–9.
Olson OE, Palmer IS, Cary EE. Modification of the official fluorometric method for selenium in plants. J Am Heart Assoc. 1975;58:117–21.
Müller-Röver S, et al. A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages. J Invest Dermatol. 2001;117:3–15.
Zhao T. Hygienic standard for cosmetics health. Beijing; 2007.
Pence BC, Delver E, Dunn DM. Effects of dietary selenium on UVB-induced skin carcinogenesis and epidermal antioxidant status. J Invest Dermatol. 1994;102:759–61.
Harvey H, Ju SJ, Son S, Feinberg L, Shaw C, Peterson W. The biochemical estimation of age in Euphausiids: laboratory calibration and field comparisons. Deep-Sea Res Part II. 2010;57(7):663–71.
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248–54.
Mei L, et al. Bioconjugated nanoparticles for attachment and penetration into pathogenic bacteria. Biomaterials. 2013;34:10328–37.
Zheng SY, et al. PEG-nanolized ultrasmall selenium nanoparticles overcome drug resistance in hepatocellular carcinoma HepG2 cells through induction of mitochondria dysfunction. Int J Nanomed. 2012;7:3939–49.
Huang DJ, Ou BX, Prior R. The chemistry behind antioxidant capacity assays. J Agr Food Chem. 2005;53:1841–56.
Sambuy Y, De Angelis I, Ranaldi G, Scarino ML, Stammati A, Zucco F. The Caco-2 cell line as a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell functional characteristics. Cell Biol Toxicol. 2005;21:1–26.
Lewinski N, Colvin V, Drezek R. Cytotoxicity of nanoparticles. Small. 2008;4:26–49.
Chen TF, Wong YS, Zheng WJ, Bai Y, Huang L. Selenium nanoparticles fabricated in Undaria pinnatifida polysaccharide solutions induce mitochondria-mediated apoptosis in A375 human melanoma cells. Colloid Surf B. 2008;67:26–31.
Feng YX, et al. Differential effects of amino acid surface decoration on the anticancer efficacy of selenium nanoparticles. Dalton Trans. 2014;3:1854–61.
Daniels LA. Elenium metabolism and bioavailability. Biol Trace Elem Res. 1996;54:185–99.
Kennedy GL, Ferenz RL, Burgess BA. Estimation of acute oral toxicity in rates by determination of the approximate lethal dose rather than the LD50. J Appl Toxicol. 1986;6:145–8.
Zhou X, et al. Enhancement of endogenous defenses against ROS by supra-nutritional level of selenium is more safe and effective than antioxidant supplementation in reducing hypertensive target organ damage. Med Hypotheses. 2007;68:952–95.