Antioxidant, Anti-Nephrolithe Activities and in Vitro Digestibility Studies of Three Different Cyanobacterial Pigment Extracts

Marine Drugs - Tập 13 Số 8 - Trang 5384-5401
Chetan Paliwal1, Tonmoy Ghosh1, Khushbu Bhayani2, Rahulkumar Maurya1, Sandhya Mishra1
1Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India
2Salt and Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India

Tóm tắt

Phycobiliprotein-containing water and carotenoid-containing methanolic extracts of three different cyanobacteria, Pseudanabaena sp., Spirulina sp. and Lyngbya sp., were studied for their DPPH scavenging, iso-bolographic studies, and anti-nephrolithe activities. The best EC50 values for DPPH scavenging were in Lyngbya water (LW, 18.78 ± 1.57 mg·mg−1 DPPH) and Lyngbya methanol (LM, 59.56 ± 37.38 mg·mg−1 DPPH) extracts. Iso-bolographic analysis revealed most of the combinations of extracts were antagonistic to each other, although LM—Spirulina methanol (SM) 1:1 had the highest synergistic rate of 86.65%. In vitro digestion studies showed that DPPH scavenging activity was considerably decreased in all extracts except for Pseudanabaena methanol (PM) and LM after the simulated digestion. All of the extracts were effective in reducing the calcium oxalate crystal size by nearly 60%–65% compared to negative control, while PM and Spirulina water (SW) extracts could inhibit both nucleation and aggregation of calcium oxalate by nearly 60%–80%.

Từ khóa


Tài liệu tham khảo

Shishido, 2015, Antifungal compounds from cyanobacteria, Mar. Drugs, 13, 2124, 10.3390/md13042124

Guschina, 2006, Lipids and lipid metabolism in eukaryotic algae, Prog. Lipid Res., 45, 160, 10.1016/j.plipres.2006.01.001

Miyamoto, K. (1997). Renewable Biological Systems for Alternative Sustainable Energy Production, FAO.

Sekar, 2008, Phycobiliproteins as a commodity: Trends in applied research, patents and commercialization, J. Appl. Phycol., 20, 113, 10.1007/s10811-007-9188-1

Fabregas, 1990, Vitamin content of four marine microalgae. Potential use as source of vitamins in nutrition, J. Ind. Microbiol., 5, 259, 10.1007/BF01569683

Mishra, 2011, Characterization of extracellular polymeric substances produced by micro-algae Dunaliella salina, Carbohydr. Polym., 83, 852, 10.1016/j.carbpol.2010.08.067

Bryant, 2004, Carotenoids in cyanobacteria, The Molecular Biology of Cyanobacteria, Volume 1, 559, 10.1007/978-94-011-0227-8_18

Koller, 2014, Microalgae as versatile cellular factories for valued products, Algal Res., 6, 52, 10.1016/j.algal.2014.09.002

Blanco, 2007, Outdoor cultivation of lutein-rich cells of Muriellopsis sp. in open ponds, Appl. Microbiol. Biotechnol., 73, 1259, 10.1007/s00253-006-0598-9

Sonani, 2014, Phycoerythrin extends life span and health span of Caenorhabditis elegans, AGE, 36, 1, 10.1007/s11357-014-9717-1

Sonani, 2014, Concurrent purification and antioxidant activity of phycobiliproteins from Lyngbya sp. A09DM: An antioxidant and anti-aging potential of phycoerythrin in Caenorhabditis elegans, Process Biochem., 49, 1757, 10.1016/j.procbio.2014.06.022

Wang, 2009, Photodynamic effect of two kinds of phycobiliproteins on human liver cancer cell line SMMC-7721 in vitro, Sheng Wu Gong Cheng Xue Bao, 25, 1417

Zhang, 2012, Antioxidant properties of polysaccharide from the brown seaweed Sargassum graminifolium (Turn.), and its effects on calcium oxalate crystallization, Mar. Drugs, 10, 119, 10.3390/md10010119

McClements, 2009, Structural design principles for delivery of bioactive components in nutraceuticals and functional foods, Crit. Rev. Food Sci. Nutr., 49, 577, 10.1080/10408390902841529

Wang, 2012, Preliminary study into the factors modulating β-carotene micelle formation in dispersions using an in vitro digestion model, Food Hydrocoll., 26, 427, 10.1016/j.foodhyd.2010.11.018

Golding, 2010, The influence of emulsion structure and stability on lipid digestion, Curr. Opin. Coll. Interface Sci., 15, 90, 10.1016/j.cocis.2009.11.006

Morist, 2001, Recovery and treatment of Spirulina platensis cells cultured in a continuous photobioreactor to be used as food, Process Biochem., 37, 535, 10.1016/S0032-9592(01)00230-8

Mishra, 2011, Preparation of highly purified C-phycoerythrin from marine cyanobacterium Pseudanabaena sp., Protein Expr. Purif., 80, 234, 10.1016/j.pep.2011.06.016

Rodriguez, 1989, Nitrogen-fixing cyanobacterium with a high phycoerythrin content, Appl. Environ. Microbiol., 55, 758, 10.1128/aem.55.3.758-760.1989

Aakermann, 1992, A comparison of the carotenoids of strains of Oscillatoria and Spirulina (Cyanobacteria), Biochem. Syst. Ecol., 20, 761, 10.1016/0305-1978(92)90035-C

Singh, O.A. (2015). Diversity Analysis and PCR Based Molecular Characterization of Carotenoid Rich Oscillatorian Cyanobacteria of North Eastern (NE) Region of India. [Ph.D. Thesis, Assam University].

Jiang, 2015, The evaluation of antioxidant interactions among 4 common vegetables using isobolographic analysis, J. Food Sci., 80, C1162, 10.1111/1750-3841.12896

Machu, 2015, Phenolic content and antioxidant capacity in algal food products, Molecules, 20, 1118, 10.3390/molecules20011118

Ismaiel, 2014, Antioxidants characterization in selected cyanobacteria, Ann. Microbiol., 64, 1223, 10.1007/s13213-013-0763-1

Dai, 2010, Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties, Molecules, 15, 7313, 10.3390/molecules15107313

Ganesan, 2008, Antioxidant properties of methanol extract and its solvent fractions obtained from selected Indian red seaweeds, Bioresour. Technol., 99, 2717, 10.1016/j.biortech.2007.07.005

Kumar, 2011, Minerals, PUFAs and antioxidant properties of some tropical seaweeds from Saurashtra coast of India, J. Appl. Phycol., 23, 797, 10.1007/s10811-010-9578-7

Yahia, 2014, Antioxidant activity and content of chlorophylls and carotenoids in raw and heat-processed Jalapeño peppers at intermediate stages of ripening, Food Chem., 146, 188, 10.1016/j.foodchem.2013.09.060

Wang, 2014, Structural characterization and bioactivity of released exopolysaccharides from Lactobacillus plantarum 70810, Int. J. Biol. Macromol., 67, 71, 10.1016/j.ijbiomac.2014.02.056

Babica, 2009, Toxins produced in cyanobacterial water blooms—Toxicity and risks, Interdiscip. Technol., 2, 36

Hall, 1990, Natural Toxins from Cyanobacteria (Blue-Green Algae), Marine Toxins, Volume 418, 87, 10.1021/bk-1990-0418.ch006

Wu, 2015, Effects of physicochemical factors and in vitro gastrointestinal digestion on antioxidant activity of R-phycoerythrin from red algae Bangia fusco-purpurea, Int. J. Food Sci. Technol., 50, 1445, 10.1111/ijfs.12775

Queiroz, 2013, Evaluation of sulfated polysaccharides from the brown seaweed Dictyopteris justii as antioxidant agents and as inhibitors of the formation of calcium oxalate crystals, Molecules, 18, 14543, 10.3390/molecules181214543

Patel, 2005, Purification and characterization of C-Phycocyanin from cyanobacterial species of marine and freshwater habitat, Protein Expr. Purif., 40, 248, 10.1016/j.pep.2004.10.028

Pancha, 2014, Nitrogen stress triggered biochemical and morphological changes in the microalgae Scenedesmus sp. CCNM 1077, Bioresour. Technol., 156, 146, 10.1016/j.biortech.2014.01.025

Bennett, 1973, Complementary chromatic adaptation in a filamentous blue-green alga, J. Cell Biol., 58, 419, 10.1083/jcb.58.2.419

Andersson, 2011, Carotenoid content and composition in rose hips (Rosa spp.) during ripening, determination of suitable maturity marker and implications for health promoting food products, Food Chem., 128, 689, 10.1016/j.foodchem.2011.03.088

Vitrac, 2009, Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays, J. Agric. Food Chem., 57, 1768, 10.1021/jf803011r

Banjanac, 2014, Centauries as underestimated food additives: Antioxidant and antimicrobial potential, Food Chem., 147, 367, 10.1016/j.foodchem.2013.10.007

Hur, 2009, Influence of initial emulsifier type on microstructural changes occurring in emulsified lipids during in vitro digestion, Food Chem., 114, 253, 10.1016/j.foodchem.2008.09.069