Antimicrobial activities of essential oils from three species of citrus fruits against six infectious tropical bacteria: implications for the care and control of common bacterial tropical diseases in developing countries

Springer Science and Business Media LLC
Ayomadewa Mercy Olatunya, Oladele Simeon Olatunya, Gul Hassan, Lateef A. Adeyemi, Oyeku Akibu Oyelami, E. T. Akintayo

Tóm tắt

AbstractThe burden of bacterial infections and the failure of antimicrobial agents are increasing worldwide, especially in sub-Saharan Africa. This raises the need for novel therapeutic agents to combat bacterial infectious diseases. The sensitivity of several common pathogenic bacteria to essential oils from the peels of three species of citrus plants was investigated. Essential oils (EOs) were extracted from the fresh and dried peels of the citrus species using standard techniques. The antimicrobial activities of the EOs were determined using agar well diffusion and broth microdilution methods, and the activities of the EOs were compared to those of twelve conventional antibiotics. The results showed that the essential oils had high inhibitory effects. The minimum inhibitory concentrations (MICs) of the EOs ranged between 0.025 and 0.15 mg/ml. The activities of the EOs against the tested microorganisms were comparable to those of conventional antibiotics. Processing techniques did not affect the antibacterial activities of the EOs. Thus, the essential oils from the citrus species used against the investigated microorganisms were comparable to conventional antibiotics, and these findings showed that the oils could be widely applied in the health and pharmaceutical industries, where they could help in combating the menace of bacterial infections and therapeutic failure from drug resistance worldwide and especially in developing countries with limited resources available to combat these infections.

Từ khóa


Tài liệu tham khảo

Murray CJ, Ikuta KS, Sharara F, Swetschinski L, Aguilar GR, Gray A, Han C, Bisignano C, Rao P, Wool E. Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. Lancet. 2022;399:629–55. https://doi.org/10.1016/S0140-6736(21)02724-0.

ECDC (European Centre for Disease Prevention and Control). Assessing the health burden of infections with antibiotic-resistant bacteria in the EU/EEA, 2016–2020. European Centre for Disease Prevention and Control, Stockholm. 2022. https://www.ecdc.europa.eu. Accessed 11 June 2023.

WHO, World Health Organization. Antimicrobial resistance. 2021. https://www.who.int/news-room/fact-sheets/detail/antimicrobial-resistance. Accessed 22 Aug 2022.

Roser M, Ritchie H. Burden of disease. Our world in data. 2021. https://ourworldindata.org/burden-of-disease Accessed 21 Aug 2022.

Olatunya AM, Akintayo ET. Evaluation of the effect of drying on the chemical composition and antioxidant activity of the essential oil of peels from three species of citrus group. Int Food Res J. 2017;24:1991–7.

Bora H, Kamle M, Mahato DK, Tiwari P, Kumar P. Citrus essential oils (CEOs) and their applications in food: an overview. Plants. 2020;9:357. https://doi.org/10.3390/plants9030357.

Blowman K, Magalhães M, Lemos MFL, Cabral C, Pires IM. Anticancer properties of essential oils and other natural products. Evid Based Complement Alternat Med. 2018. https://doi.org/10.1155/2018/3149362.

Fokou JBH, Dongmo PMJ, Boyom FF. Essential oil’s chemical composition and pharmacological properties. In: El-Shemy HA, editor. Essential oils. Rijeka: IntechOpen; 2020.

Lawani BT, Bayode MT, Sadibo ME, Awodire EF, Aro OP, Akindele AA. Antibiotic resistance microbes’ (ARM) mechanisms and management: a phytomedicinal approach. Proc Natl Acad Sci India Sect B Biol Sci. 2023. https://doi.org/10.1007/s40011-023-01525-9.

Babatola AO, Fadare JO, Olatunya OS, Obiako R, Enwere O, Kalungia A, Ojo TO, Sunmonu TA, Desalu O, Godman B. Addressing antimicrobial resistance in Nigerian hospitals: exploring physicians prescribing behavior, knowledge, and perception of antimicrobial resistance and stewardship programs. Expert Rev Anti Infect Ther. 2021;19:537–46. https://doi.org/10.1080/14787210.2021.1829474.

Seukep AJ, Mbuntcha HG, Zeuko’o EM, Woquan LS, Nembu NE, Bomba FT, Watching D, Kuete V. Established antibacterial drugs from plants. Adv Bot Res. 2023;106:81–149.

Seukep AJ, Nembu NE, Mbuntcha HG, Kuete V. Bacterial drug resistance towards natural products. Adv Bot Res. 2023;106:21–45.

European Directorate for the Quality of Medicines & HealthCare. European Pharmacopoeia. European Directorate for the Quality of Medicines & HealthCare, Strasbourg. 2007. https://www.edqm.eu. Accessed 12 June 2023.

Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, Morgan DR. Manual of clinical microbiology (6th edn). Trends Microbiol. 1995;3:449–449.

CLSI (Clinical and Laboratory Standards Institute). Performance standards for antimicrobial susceptibility testing (No. M100), 30th edn CLSI Supplement M100. Clinical and Laboratory Standards Institute, Wayne, PA. 2020.

Bouhdid S, Skali SN, Idaomar M, Zhiri A, Bandoux D, Amensour M, Abrini J. Antibacterial and antioxidant activities of Origanum compactum essential oil. Afr J Biotechnol. 2008. https://doi.org/10.4314/AJB.V7110.58723.

Moukhles A, Ellaghdach A, Driss AB, El Amrani MA, Aghmiz A, Mansour AL. Chemical profile and in vitro antibacterial potential of essential oils and hydrolat extracts from aerial parts of three wild species of Moroccan Thymus. Sci Afr. 2022. https://doi.org/10.1016/j.sciaf.2022.e01434.

Hafidh RR, Abdulamir AS, Vern LS, Bakar FA, Abas F, Jahanshiri F, Sekawi Z. Inhibition of growth of highly resistant bacterial and fungal pathogens by a natural product. Open Microbiol J. 2011;5:96–106. https://doi.org/10.2174/1874285801105010096.

Oyelami OA, Agbakwuru EA, Adeyemi LA, Adedeji GB. The effectiveness of grapefruit (Citrus paradisi) seeds in treating urinary tract infections. J Altern Complement Med. 2005;11:369–71. https://doi.org/10.1089/acm.2005.11.369.

Adegoke SA, Oyelami OA, Olatunya OS, Adeyemi LA. Effects of lime juice on malaria parasite clearance. Phytother Res. 2011;25:1547–50. https://doi.org/10.1002/ptr.3418.

Guo Y, Song G, Sun M, Wang J, Wang Y. Prevalence and therapies of antibiotic-resistance in Staphylococcus aureus. Front Cell Infect Microbiol. 2020;10:107. https://doi.org/10.3389/fcimb.2020.00107.

Al-Jabri NN, Hossain MA. Comparative chemical composition and antimicrobial activity study of essential oils from two imported lemon fruits samples against pathogenic bacteria. Beni Suef Univ J Basic Appl Sci. 2014;3:247–53. https://doi.org/10.1016/j.bjbas.2014.10.011.

Mahmud S, Saleem M, Siddique S, Ahmed R, Khanum R, Perveen Z. Volatile components, antioxidant and antimicrobial activity of Citrus acida var. sour lime peel oil. J Saudi Chem Soc. 2009;13:195–8. https://doi.org/10.1016/j.jscs.2009.03.001.

Bayode MT, Olalemi AO, Oladejo BO. Multiple antibiotic resistant index and detection of qnrS and qnrB genes in bacterial consortium of urine samples from clinical settings. Eur J Biol Res. 2021;11:45–56. https://doi.org/10.5281/zenodo.4304311.

Langendonk RF, Neill DR, Fothergill JL. The building blocks of antimicrobial resistance in Pseudomonas aeruginosa: implications for current resistance-breaking therapies. Front Cell Infect Microbiol. 2021;11:665759. https://doi.org/10.3389/fcimb.2021.665759.

Man A, Santacroce L, Iacob R, Mare A, Man L. Antimicrobial activity of six essential oils against a group of human pathogens: a comparative study. Pathogens. 2019;8:15. https://doi.org/10.3390/pathogens8010015.

Cazella LN, Glamoclija J, Soković M, Gonclaves JE, Linde GA, Colauto NB, Gazim ZC. Antimicrobial activity of essential oil of Baccharis dracunculifolia DC (Asteraceae) aerial parts at flowering period. Front Plant Sci. 2019;10:27. https://doi.org/10.3389/fpls.2019.00027.

Shahzad K, Nawaz S, Ahmad R, Akram N, Iqbal Z. Evaluation of antbacterial, antifungal and antioxidant activity of essentail oil of Citrus reticulata fruit (tangerine fruit peel). Pharmacologyonline. 2009;3:614–22.

Değirmenci H, Erkurt H. Relationship between volatile components, antimicrobial and antioxidant properties of the essential oil, hydrosol and extracts of Citrus aurantium L. flowers. J Infect Public Health. 2020;13:58–67. https://doi.org/10.1016/j.jiph.2019.06.017.

Javed S, Ahmad R, Shahzad K, Nawaz S, Saeed S, Saleem Y. Chemical constituents, antimicrobial and antioxidant activity of essential oil of Citrus limetta var. Mitha (sweet lime) peel in Pakistan. Afr J Microbiol Res. 2013;7:3071–7. https://doi.org/10.5897/AJMR12.1254.

Thông tin
Thông tin xuất bản

Springer Science and Business Media LLC

Thông tin tác giả